Biosynthesis of silver nanoparticles from mangrove plant (Avicennia marina) extract and their potential mosquito larvicidal property
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To identify the larvicidal activities of silver nanoparticles synthesised with Avicennia marina leaf extract against the larvae of Aedes aegypti and Anopheleus stephensi, in vitro larvicidal activities such as LC50 and LC90 were assessed. Further, characterisation such as UV and FTIR analysis were carried out for the synthesised silver nanoparticles. The LC50 value of the synthesised silver nanoparticles was identified as 4.374 and 7.406 mg/L for An. stephensi and Ae. aegypti larvae respectively. Further, the LC90 values are also identified as 4.928 and 9.865 mg/L for An. stephensi and Ae. aegypti species respectively. The synthesised silver nanoparticles have maximum absorption at 420 nm with the average size of 60–95 nm. The FTIR data showed prominent peaks in (3940.57, 3929.00, 3803.63, 3712.97, 2918.30, 2231.64, 1610.50, 1377.17, 1257.59, 1041.59, 1041.56, 775.38, 667.37 and 503.21) different ranges. The biosynthesis of silver nanoparticles with leaf aqueous extract of A. marina provides potential source for the larvicidal activity against mosquito borne diseases. The present study proved the mosquitocidal properties of silver nanoparticles synthesised from mangroves of Vellar estuary. This is an ideal eco-friendly approach for the vector control programs.
KeywordsBiosynthesis AgNO3 Mangrove Larvicidal FTIR
The author thanks the authorities of Annamalai University for providing the necessary facilities and the INCOIS-SATCORE Project (G4/515/2008), Ministry of Earth Sciences (Government of India) for financial support. We also thank the anonymous referees for the valuable comments, which greatly improved our manuscript.
Conflict of interest
All authors have read the manuscript and have agreed to submit it in its current form for consideration for publication in the Journal. We declare that we have no conflict of interest.
- Abeysinghe PD, Wanigatunge RP, Pathirana RN (2006) Evaluation of antibacterial activity of different mangrove plant extracts. Ruhuna J Sci 1:108–116Google Scholar
- Becker N, Petric D, Zgomba M (2003) Mosquitoes and their control. Kluwer Academic/Plenum Publishers, New York, pp 5–23Google Scholar
- Chakkaravarthy VM, Ambrose T, Vincent S (2011) Bioefficacy of Azadirachta indica (A. juss) and Datura metel (Linn.) leaves extracts in controlling Culex quinquefaciatus (Diptera: Culicidae). Trends Appl Sci Res 8:191–197Google Scholar
- Dhanasekaran D, Sakthi V, Thajuddin N (2010) Preliminary evaluation of Anopheles mosquito larvicidal efficacy of mangrove actinobacteria. Int J Appl Biol Pharm Tech 1(2):374–381Google Scholar
- Finney DJ (1971) Probit analysis. Cambridge University Press, CambridgeGoogle Scholar
- Kathiresan K, Veera Ravi A (1990) Seasonal changes in tannin content of mangrove leaves. Indian For 116(5):390–392Google Scholar
- Khafagi I, Gab-Alla A, Salama W (2003) Biological activities and phytochemical constituents of the gray mangrove Avicennia marina (Forssk.) Vierh. Egypt J Bot 5:62–69Google Scholar
- Parashar UK, Saxenaa PS, Srivastava A (2009) Bio inspired synthesis of silver nanoparticles. Dig J Nanomater Biostruct 4:159–166Google Scholar
- Rao DR, Mani TR, Rajendran R (1995) Development of high level of resistance to Bacillus sphaericus in a field population of C. quinquefasciatus Say (Diptera: Culcidae). Afr J Biomed Res 8:31–33Google Scholar
- Ravikumar S, Ramanathan G, Subhakaran M (2009) Antimicrobial compounds from marine halophytes for silkworm disease treatment. Int J Med Sci 5:184–191Google Scholar
- Service MV (1983) Biological control of mosquitoes has it a future? Mosq News 43:113–120Google Scholar
- Thangam TS, Kathiresan K (1991) Mosquito larvicidal activity of marine plant extracts with synthetic insecticides. Bot Mar 34:537–539Google Scholar
- Thangam TS, Kathiresan K, Mabbett T (1993) Mosquito larvicidal activity of seaweed extract against A. aegypti and C. quinquefasciatus. Int Pest Control 35:94–95Google Scholar
- Vinayagam A, Senthilkumar N, Umamaheshwari A (2008) Larvicidal activity of seaweed extract against A. aegypti and C. quinquefasciatus. Int Pest Control 35:94–95Google Scholar
- WHO (1975) Instructions for determining the susceptibility of resistance mosquito larvae to insecticides. Mimeographed Document WHO/VBC/75 583Google Scholar
- WHO (2010) Antiretroviral treatment working group treatment white paperGoogle Scholar
- Zandi K, Taherzadeh M, Yaghoubi R (2009) Antiviral activity of Avicennia marina against herpes simplex virus type 1 and vaccine strain of poliovirus (An in vitro study). J Med Plan Res 3(10):771–775Google Scholar