2D-PAGE analysis of the soluble proteins of the tropical liver fluke, Fasciola gigantica and biliary amphistome, Gigantocotyle explanatum, concurrently infecting Bubalus bubalis
- 140 Downloads
The digenetic trematodes, Fasciola gigantica and Gigantocotyle explanatum, belonging to the family Fasciolidae and Paramphistomidae respectively, have been often found to concurrently infect the liver of Indian water buffalo Bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. In the present study the soluble gene products of both F. gigantica and G. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. The soluble proteomic profile revealed considerable similarity as well as differences in the size, distribution pattern, total number, the isoelectric point (pI) and molecular weight (Mr) of the resolved polypeptide spots. The maximum number of polypeptide spots with a molecular weight range of >10 to 160 kDa were recorded with a pI range of 7–9 followed by pI range of 5–7, 9–10 and 3–5 in both the parasites. However, considerable variation was recorded in the Mr of the polypeptides belonging to each pI range. The genetic heterogeneity could be an obvious contributing factor for such differences but some polypeptides appeared to be conserved in the two species. The molecular similarities and the habitat preference by these worms may be a consequence of microenvironmental cues that guide these flukes to reach their habitat through different routes and establish a successful host-parasite relationship.
KeywordsDigenetic trematodes 2D PAGE Concurrent infection Bubalus bubalis
Authors are grateful to the Chairman, Department of Zoology, Aligarh Muslim University for providing the necessary facilities. Thanks are extended to University Grants Commission, New Delhi for financial assistance to YAK. Financial support under the BBSRC UK CIDLID project to SMAA and fellowship to MAHK is also gratefully acknowledged.
- Anon (2011–2012) Annual report—Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Government of India, pp 1–112 (Gives data on both Fasiolosis and amphistomosis)Google Scholar
- Nizami WA, Khan P, Abidi SMA (1991) Problems and prospects of buffalo amphistomosis. Parasit Hung 24:69–79Google Scholar
- Osikovski E, Kamburov P, Vasilev I (1978) Comparative electrophoretic study of the water-soluble proteins of some representative of genus Paraamphistomum. Khelminthologiye Sofia 6:68–74Google Scholar
- Saifullah MK, Abidi SMA, Nizami WA (2001) Polypeptide profile of in vitro released eggs of some digenetic trematodes. J Vet Parasit 15(1):25–28Google Scholar
- Simpson AJG (1986) The influence of molecular heterogeneity in helminth identification, protective immunity and Immunodiagnosis. In: Parasitology-Quo Vadit? Proceedings of the sixth int cong parasitol, Brisbane, AustraliaGoogle Scholar