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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 901–905 | Cite as

Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

  • Marwa M. I. Ghallab
  • Inas Z. Abdel Aziz
  • Eman Y. Shoeib
  • Ayman A. El-Badry
Original Article

Abstract

Cryptosporidium is a significant cause of diarrhea worldwide especially in children. Infection may end fatally in immunocompromised patients. Multi-attribute analysis was used to determine the lab utility of 4 diagnostics; coproscopy of AF stained fecal smear, fecal immunoassays by ICT and ELISA and copro-nPCR assay targeting Hsp90 gene, for detection of Cryptosporidium in stool of 250 Egyptian children (150 diarrheic and 100 non-diarrhaeic children). Also, to determine Cryptosporidium molecular prevalence. Cryptosporidium was an important enteric pathogen among both diarrheic and non-diarrheic study children with a clearly high prevalence of 16.4 % (n = 41). Conventional methods had perfect specificity (100 %) but couldn`t be used as a consistent single detection method due to their lowered sensitivities. Multi-attribute analysis ranked nPCR the highest test for lab use. Being the test with the best diagnostic yield, nPCR is a reliable diagnostic test and is going to replace conventional methods for reliable detection of Cryptosporidium.

Keywords

Cryptosporidium Hsp90 Copro-DNA Nested PCR Multi-attribute Fecal immunoassay ELISA ICT AF stain 

Notes

Conflict of interest

The authors declare that they have no Conflict of interests.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Marwa M. I. Ghallab
    • 1
  • Inas Z. Abdel Aziz
    • 1
  • Eman Y. Shoeib
    • 1
  • Ayman A. El-Badry
    • 1
  1. 1.Medical Parasitology, Kasr Al-Ainy School of MedicineCairo UniversityCairoEgypt

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