Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 855–859 | Cite as

IL-4 gene expression in adventitial layer (fibrous layer) of hepatic ovine and bovine hydatid cysts

  • Zahra Dorosti
  • Sepideh Tolouei
  • Hossein Khanahmad
  • Rasool Jafari
  • Fereshteh Jafaee
  • Seyedeh Marayam Sharafi
  • Hossein Yousofi DaraniEmail author
Original Article


Cystic Echinococcosis is a parasitic disease with cosmopolitan distribution caused by the tape worm Echinococcus granulosus. Fibrous layer is developed around the cyst as a host immune response reaction. The aim of this study was to evaluate the rate of IL-4 gene expression in fibrous layer of bovine and ovine hepatic hydatid cysts using quantitative technique of Real-Time PCR. In this descriptive study the samples of hydatid cyst fibrous layer were taken from 6 bovine and 6 ovine hepatic hydatid cysts. Samples of normal liver tissue close to the cyst were also taken as controls. Total RNA from each sample was extracted and then converted to cDNA. Afterward, the rate of IL-4 gene expression for each sample was evaluated using real-time PCR technique. Data were analyzed by REST software (version 2.0.13, 2009). In sheep the rate of IL-4 gene expression in the fibrous layer of hepatic hydatid cysts was 1.98 times more than the rate of IL4 gene expression in control samples, but the difference was not significant (P = 0.561). In cattle the rate of IL-4 gene expression in the fibrous layer of hepatic hydatid cysts was 9.84 times more than that of control samples which was statistically significant (P < 0.001). With high rate of IL4 expression especially in fibrous layer of bovine hydatid cyst, it can be concluded that this interleukin may play an important role in host parasite relationship.


Hydatid cyst IL-4 Gene expression Real-Time PCR Fibrous layer 



The authors would like to acknowledge Vice-chancellor of Research and Technology, Isfahan University of Medical Sciences for financial support of this work.

Conflict of interest

The authors have no conflict of interests.


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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Zahra Dorosti
    • 1
  • Sepideh Tolouei
    • 1
  • Hossein Khanahmad
    • 2
  • Rasool Jafari
    • 1
  • Fereshteh Jafaee
    • 1
  • Seyedeh Marayam Sharafi
    • 3
  • Hossein Yousofi Darani
    • 1
    • 3
    Email author
  1. 1.Department of Parasitology and Mycology, School of MedicineIsfahan University of Medical SciencesIsfahanIran
  2. 2.Department of Genetics and Molecular Biology, School of MedicineIsfahan University of Medical ScienceIsfahanIran
  3. 3.Infectious and Tropical Diseases Research CenterIsfahan University of Medical SciencesIsfahanIran

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