Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 845–847 | Cite as

Seroprevalence of Neospora caninum infection in free ranging chickens (Gallus domesticus)

  • M. Sayari
  • M. Namavari
  • S. Mojaver
Original Article


Recently chickens are considered as an important intermediate hosts for Neospora caninum. Free range chickens expose to infection with N. caninum oocysts because they feed from the ground therefore they could be a good index of the environmental contamination. We studied N. caninum infection in free range chickens by serological. One hundred and fifty chickens purchased from five regions from Fars province and their blood were used for serological testing. Antibodies to N. caninum were found in 26 (17.33 %) of 150 serum samples by MAT. This study is the first to describe the presence of antibodies to N. caninum in chicken in Iran. These serological results indicate a widespread exposure of free range chickens to N. caninum in south of Iran.


Neospora caninum Seroprevalence Chicken Modified agglutination test (MAT) 


  1. Bartels CJ, Wouda W, Schukken YH (1999) Risk factors for Neospora caninum-associated abortion storms in dairy herds in The Netherlands. Theriogenology 52:247–257CrossRefPubMedGoogle Scholar
  2. Costa KS, Santos SL, Uzeda RS, Pinheiro AM, Almeida MA, Araujo FR, McAllister MM, Gondim LF (2008) Chickens (Gallus domesticus) are natural intermediate hosts of Neospora caninum. Int J Parasitol 38:157–159CrossRefPubMedGoogle Scholar
  3. Darwich L, Cabezon O, Echeverria I, Pabon M, Marco I, Molina-Lopez R, Alarcia-Alejos O, Lopez-Gatius F, Lavin S, Almeria S (2012) Presence of Toxoplasma gondii and Neospora caninum DNA in the brain of wild birds. Vet Parasitol 183:377–381CrossRefPubMedGoogle Scholar
  4. Dubey JP, Graham DH, Blackston CR, Lehmann T, Gennari SM, Ragozo AM, Nishi SM, Shen SK, Kwok OC, Hill DE, Thulliez P (2002) Biological and genetic characterisation of Toxoplasma gondii isolates from chickens (Gallus domesticus) from Sao Paulo, Brazil: unexpected findings. Int J Parasitol 32:99–105CrossRefPubMedGoogle Scholar
  5. Frenkel JK, Smith DD (2003) Determination of the genera of cyst forming coccidia. Parasitol Res 91:384–389CrossRefPubMedGoogle Scholar
  6. Furuta PI, Mineo TW, Carrasco AO, Godoy GS, Pinto AA, Machado RZ (2007) Neospora caninum infection in birds: experimental infections in chicken and embryonated eggs. Parasitology 134:1931–1939CrossRefPubMedGoogle Scholar
  7. Gondim LF, Pinheiro AM, Santos PO, Jesus EE, Ribeiro MB, Fernandes HS, Almeida MA, Freire SM, Meyer R, McAllister MM (2001) Isolation of Neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. Vet Parasitol 101:1–7CrossRefPubMedGoogle Scholar
  8. Gondim LS, Abe-Sandes K, Uzeda RS, Silva MS, Santos SL, Mota RA, Vilela SM, Gondim LF (2010) Toxoplasma gondii and Neospora caninum in sparrows (Passer domesticus) in the Northeast of Brazil. Vet Parasitol 168:121–124CrossRefPubMedGoogle Scholar
  9. Khodakaram-Tafti A, Mansourian M, Namavari M, Hosseini A (2012) Immunohistochemical and polymerase chain reaction studies in Neospora caninum experimentally infected broiler chicken embryonated eggs. Vet Parasitol 188:10–13CrossRefPubMedGoogle Scholar
  10. Lundén A, Marks J, Maley SW, Innes EA (1998) Cellular immune responses in cattle experimentally infected with Neospora caninum. Parasite Immunol 20:519–526CrossRefPubMedGoogle Scholar
  11. Mansourian M, Khodakaram-Tafti A, Namavari M (2009) Histopathological and clinical investigations in Neospora caninum experimentally infected broiler chicken embryonated eggs. Vet Parasitol 166:185–190CrossRefPubMedGoogle Scholar
  12. Martins J, Kwok OC, Dubey JP (2011) Seroprevalence of Neospora caninum in free-range chickens (Gallus domesticus) from the Americas. Vet Parasitol 182:349–351CrossRefPubMedGoogle Scholar
  13. McAllister MM, Dubey JP, Lindsay DS, Jolley WR, Wills RA, McGuire AM (1998) Dogs are definitive hosts of Neospora caninum. Int J Parasitol 28:1473–1478CrossRefPubMedGoogle Scholar
  14. Mineo TW, Carrasco AO, Raso TF, Werther K, Pinto AA, Machado RZ (2011) Survey for natural Neospora caninum infection in wild and captive birds. Vet Parasitol 182:352–355CrossRefPubMedGoogle Scholar
  15. Otranto D, llazari A, Testini G, Traversa D, Frangipane di Regalbono A, Badan M, Capelli G (2003) Seroprevalence and associated risk factors of neosporosis in beef and dairy cattle in Italy. Vet Parasitol, Amsterdam 118(1–2):7–18CrossRefGoogle Scholar
  16. Packham AE, Sverlow KW, Conrad PA, Loomis EF, Rowe JD, Anderson ML, Marsh AE, Cray C, Barr BC (1998) A modified agglutination test for Neospora caninum: development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 5:467–473PubMedPubMedCentralGoogle Scholar
  17. Trees AJ, Davidson HC, Innes E, Wastling JM (1999) Toward evaluating the economic impact of bovine neosporosis. Int J Parasitol 29:1195–1200CrossRefPubMedGoogle Scholar

Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  1. 1.Departments of Pathobiology, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Razi Vaccine and Serum Research InstituteShirazIran

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