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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 840–844 | Cite as

Pathobiology of human RH strain induced experimental toxoplasmosis in murine model

  • Vikrant Sudan
  • A. K. Tewari
  • Harkirat Singh
  • R. Singh
Original Article

Abstract

Of late, toxoplasmosis has gained immense importance as an opportunist parasite in immunocompromised patients. In immunocompromised subjects, the disease is supposed to occur in acute form and causes acute toxoplasmic encephalitis. However, the exact pathogenesis of other vital organs, particularly in acute form of infection, is still a matter of debate. Therefore, an attempt was made to study the pathogenesis of acute form of toxoplasmosis using cryopreserved human RH strain of the parasite in murine models. For this, 100 tachyzoites were given to individual mice and upon the setup of acute form of infection, the mice were euthanized and the organs were processed for histopathology. Histopathology revealed tachyzoites in liver only while severe necrosis due to multiplication of tachyzoites were visible in liver, spleen, lungs and brain. Kidneys and heart appeared more or less normal. Finally, the pathology of disease in these organs is described in detail. The present research has generated some vital information regarding necrotic changes in tissues due to acute toxoplasmosis and will defiantly help the researchers in the better understanding of disease particularly in humans and putting up of suitable treatment regime for human subjects infected with acute toxoplasmosis.

Keywords

Experimental toxoplasmosis Histopathology Murine model RH stain 

Notes

Acknowledgments

The authors are thankful to the Director, IVRI for providing the facilities and to the ICAR for the fellowship awarded to the first author during the perusal of his master’s programme.

Declaration

The experiments to the laboratory animal were done as per the approval of University Ethical Committee.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Vikrant Sudan
    • 1
    • 2
  • A. K. Tewari
    • 3
  • Harkirat Singh
    • 1
    • 4
  • R. Singh
    • 5
  1. 1.Indian Veterinary Research Institute (IVRI)IzatnagarIndia
  2. 2.Department of Veterinary Parasitology, College of Veterinary Sciences & Animal HusbandryU. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)MathuraIndia
  3. 3.Department of ParasitologyIVRIIzatnagarIndia
  4. 4.Department of ParasitologyGADVASULudhianaIndia
  5. 5.CADRADIVRIIzatnagarIndia

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