A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides
As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications was the main objective of present study.
KeywordsMalaria Plasmodium falciparum Plasmodium vivax DNA extraction Fixed Giemsa-stained Blood slide
Our appreciation and thanks to Mr Masoud Yarian, head of Minab malaria lab for supporting samples collection, Isfahan University of Medical Sciences and Islamic Azad University of Falavarjan Branch for some support of this study.
- Eskandarian AA, Keshavarz H, Mahboudi F (2003) Pyrimethamine and proguanil resistance-conferring key mutations in dehydrofolate reductase gene of Plasmodium falciparum isolates from Iran. Iranian J Publ Health 32(4):8–12Google Scholar
- Gilles H (1993) Diagnostic methods in malaria. In: Gilles H, Warrel D (eds) Bruce-Chwatt’s essential malariology, 3rd edn. Edward Arnold, London, pp 78–95Google Scholar
- World Health Organization, 1996. Fact Sheet No. 94. Geneva:World Health OrganizationGoogle Scholar