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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 805–812 | Cite as

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

  • Adel Farghaly
  • Ayman A. Saleh
  • Soad Mahdy
  • Dalia Abd El-Khalik
  • Naglaa F. Abd El-Aal
  • Sara A. Abdel-Rahman
  • Marwa A. Salama
Original Article

Abstract

The present study aimed to evaluate a polymerase chain reaction (PCR) assay used for detection of Schistosoma mansoni infection in Biomphalaria alexandrina snails in early prepatent period and to compare between it and the ordinary detection methods (shedding and crushing). Biomphalaria alexandrina snails are best known for their role as intermediate hosts of S. mansoni. DNA was extracted from infected snails in addition to non-infected “negative control” (to optimized the efficiency of PCR reaction) and subjected to PCR using primers specific to a partial sequence of S. mansoni fructose-1,6-bus phosphate aldolase (SMALDO). SMALDO gene was detected in the infected laboratory snails with 70, 85, and 100 % positivity at the 1st, 3rd, and 7th day of infection, respectively. In contrast, the ordinary method was not sensitive enough in detection of early prepatent infection even after 7 days of infection which showed only 25 % positivity. By comparing the sensitivity of the three methods, it was found that the average sensitivity of shedding method compared to PCR was 23.8 % and the average sensitivity of crushing method compared to PCR was 46.4 % while the sensitivity of PCR was 100 %. We conclude that PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. This can help in detection of the areas and times of high transmission which in turn will be very beneficial in planning of the exact timing of the proper control strategy.

Keywords

SMALDO PCR S. mansoni Prepatent infection 

References

  1. Abath FG, Gomes Al, Melo Fl, Barbosa CS, Werkhauser RP (2006) Molecular approaches for the detection of Schistosoma mansoni: possible applications in the detection of snail infection, monitoring of transmission sites and diagnosis of human infection. Mem Inst Oswaldo Cruz Rio de Janeiro 101(Suppl. I):145–148CrossRefGoogle Scholar
  2. Abbasi I, King CH, Muchiri EM, Hamburger J (2010) Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. Am J Trop Med Hyg 83(2):427–432CrossRefPubMedPubMedCentralGoogle Scholar
  3. Abu El Einin HM, Mansour WA, El-Dabaa E (2009) Assessment of infected Biomphalaria alexandrina snails by detecting Schistosoma mansoni antigen and specific gene. Aust J Basic Appl Sci 3(3):2747–2753Google Scholar
  4. Behrman AJ (2005) Schistosomiasis. EMedicine. Available at: http://www.emedicine.com/emerg/topic857.htm. Accessed 31 March 2005
  5. Caldeira RL, Jannotti-Passos LK, Lira PM, Carvalho OS (2004) Diagnostic of Biomphalaria Snails and Schistosoma mansoni: DNA obtained from traces of shell organic materials. Mem Inst Oswaldo Cruz Rio de Janeiro 99(5):499–502CrossRefGoogle Scholar
  6. Chu KY, Dawood v (1970) Cercarial production from Biomphalaria alexandrina infected with Schistosoma mansoni. Bull World Health Org 42:574–596Google Scholar
  7. El-Dabaa E, Mei H, El-Sayed A, Karim AM, Eldesoky HM, Fahim FA, LoVerde PT, Saber MA (1998) Cloning and characterization of Schistosoma mansoni fructose-1,6-bisphosphate aldolase isozyme. J Parasitol 84:954–960CrossRefPubMedGoogle Scholar
  8. Fenwick A (2006) New initiatives against Africa’s worms. Trans R Soc Trop Med Hyg 100:200–207CrossRefPubMedGoogle Scholar
  9. Franco GR, Rabelo EML, Azevedo V, Pena HB, Ortega JM, Santos TM, Meira WSF, Rodrigues NA, Dias CMM, Harrop R, Wilson A, Saber MA, Abdel-Hamid H, Faria MSC, Margutti MEB, Parra JC, Pena SDJ (1997) Evaluation of cDNA libraries from different developmental stages of Schistosoma mansoni for production of expressed sequence tags (ESTs). DNA Res 4:231–240CrossRefPubMedGoogle Scholar
  10. Hahn UK, Bender RC, Bayne CJ (2001) Killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata: role of reactive oxygen species. J Parasitol 87:292–299CrossRefPubMedGoogle Scholar
  11. Hamburger J, Xin HX, Ramzy RM, Jourdane J, Ruppel A (1998) A polymerase chain reaction assay for detecting snails infected with Bilharzia parasites (Schistosoma mansoni) from very early prepatency. Am J Trop Med Hyg 59(6):872–876CrossRefPubMedGoogle Scholar
  12. Hamburger J, Hoffman O, Kariuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH (2004) Large scale polymerase chain reaction based surveillance of Schistosoma haematobium DNA in snails from transmission sites in Costal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71(6):765–773CrossRefPubMedGoogle Scholar
  13. Hamed MA (2010) Strategic control of Schistosoma intermediate host. Asian J Epidemiol 3(3):123–140CrossRefGoogle Scholar
  14. Hanelt B, Adema CM, Mansour MH, Loker ES (1997) Detection of Schistosoma mansoni in Biomphalaria using nested PCR. J Paraitol 83:387–394CrossRefGoogle Scholar
  15. Humphries J (2010) Effects of Larval Schistosomes on Biomphalaria Snails. In: Toledo R, Fried B (eds) Biomphalaria snails and larval trematodes. Springer Verlag, New York, pp 103–125Google Scholar
  16. Jannotti-Passos LK, Vidigal T, Dias-Neto E, Pena S, Simpson A, Dutra W, Souza C, Carvalho-Parra J (1997) PCR amplification of the mitochondrial DNA minisatellite region to detect Schistosoma mansoni infection in Biomphalaria glabrata snails. J Parasitol 83:395–399CrossRefPubMedGoogle Scholar
  17. Liang YS, John I, Bruce JI, David AB (1987) Laboratory cultivation of schistosome vector snails and maintenance of schistosome life cycle. Proc First Sine Am Symp 1:34Google Scholar
  18. Melo FL, Gomes AV, Barbosa CS, Werkhauser RP, Abath FGC (2006) Development of molecular approaches for the identification of transmission sites of schistosomiasis. Trans R Soc Trop Med Hyg 100:1049–1055CrossRefPubMedGoogle Scholar
  19. Morgan JA, Dejong RJ, Snyder SD, Mkoji GM, Loker ES (2001) Schistosoma mansoni and Biomphalaria: past history and future trends. Parasitology 123(7):211–228CrossRefGoogle Scholar
  20. Negrao-Correa D, Mattos AC, Pereira CA, Martins-Souza RL, Coelho PM (2012) Interaction of Schistosoma mansoni sporocysts and hemocytes of Biomphalaria. J Parasitol Res. vol 2012Google Scholar
  21. Pontes LA, Dias-Neto E, Rabello A (2002) Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces. Am J Trop Med Hyg 66:157–162CrossRefPubMedGoogle Scholar
  22. Prah SK, James C (1977) The influence of physical factors on the behavior and infectivity of miracidia of S. mansoni and S. haematobium. II- effect of temperature and ultraviolet light. Helminthology 51:73–85CrossRefGoogle Scholar
  23. Sandoval N, Siles-Lucas M, Lopez AJ, Pérez-Arellano JL, Gárate T, Muro A (2006) Schistosoma mansoni: a diagnostic approach to detect acute schistosomiasis infection in a murine model by PCR. Exp Parasitol 114:84–88CrossRefPubMedGoogle Scholar
  24. Satayathum SA, Muchiri EM, Ouma JH, Whalen CC, King CH (2006) Factors affecting infection and reinfection with Schistosoma haematobium in coastal Kenya: survival analysis during nine-year, school-based treatment program. Am J Trop Med Hyg 75:83–92CrossRefPubMedPubMedCentralGoogle Scholar
  25. Schmitt J, Wuhrer M, Hamburger J, Jourdane J, Ramzy RM, Gever R (2002) Schistosoma mansoni and Schistosoma haematobium: identification and characterization of glycoconjugate antigens in hemolymph of infected vector snails. J Parasitol 88:505–513CrossRefPubMedGoogle Scholar
  26. Webbe G (1965) Transmission of bilharziasis 2: production of cercariae. Bull World Health Orag 33:155–162Google Scholar
  27. Webbe G, El Hak S (1990) Progress in the control of schistosomiasis in Egypt. Trans R Soc Trop Med Hyg 84:394–400CrossRefPubMedGoogle Scholar
  28. World Health Organization (2013) Schistosomiasis a major public health Available: http://www.who.int/schistosomiasis/en/(http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi)

Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Adel Farghaly
    • 1
  • Ayman A. Saleh
    • 2
  • Soad Mahdy
    • 1
  • Dalia Abd El-Khalik
    • 1
  • Naglaa F. Abd El-Aal
    • 1
  • Sara A. Abdel-Rahman
    • 1
  • Marwa A. Salama
    • 1
  1. 1.Department of Medical Parasitology, Faculty of MedicineZagazig UniversityZagazigEgypt
  2. 2.Department of Animal Wealth Development, Genetics & Genetic Engineering, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt

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