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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 1087–1090 | Cite as

Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates

  • K. P. Shyma
  • S. K. Gupta
  • J. P. Gupta
  • Ajit Singh
  • S. S. Chaudhari
  • Veer Singh
Short Communication

Abstract

The differences or similarities among different isolates of Trypanosoma evansi through endonuclease profile was identified in the present study. The repetitive nuclear DNA of T. evansi isolated from infected cattle, buffalo and equine blood was initially amplified by PCR using specific primers. A panel of restriction enzymes, EcoRI, Eco91l, HindIII and PstI were for complete digestion of PCR products. Agarose gel electrophoresis of digested product did not show cleavage fragments and only single DNA band of the original size was visible in the ethidium bromide stained agarose gel. This indicated that the 227 bp PCR product from repetitive sequence had no site-specific cleavage sites for the REs used in this study. No heterogeneity in the repetitive nuclear DNA restriction endonuclease profile among the different isolates was recorded.

Keywords

Buffalo Cattle Equine PCR amplification Trypanosomes Restriction digestion 

Notes

Acknowledgments

The authors sincerely acknowledge the Dean, College of Veterinary Sciences, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar for providing necessary facilities for carrying out this work.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • K. P. Shyma
    • 1
  • S. K. Gupta
    • 3
  • J. P. Gupta
    • 2
  • Ajit Singh
    • 4
  • S. S. Chaudhari
    • 5
  • Veer Singh
    • 1
  1. 1.Department of Parasitology, College of Veterinary Sciences & Animal HusbandrySardarkrushinagar Dantiwada Agricultural UniversityGujaratIndia
  2. 2.Department of AGB, College of Veterinary Sciences & Animal HusbandrySardarkrushinagar Dantiwada Agricultural UniversityGujaratIndia
  3. 3.Departments of Veterinary Parasitology, College of Veterinary ScienceLala Lajapat Rai University of Veterinary and Animal SciencesHisarIndia
  4. 4.Department of Immunology, College of Veterinary ScienceLala Lajapat Rai University of Veterinary and Animal SciencesHisarIndia
  5. 5.Department of TVCC, College of Veterinary ScienceLala Lajapat Rai University of Veterinary and Animal SciencesHisarIndia

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