Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran
- 234 Downloads
Echinococcus granulosus as an etiologic agent of hydatid cyst is one of the most important zoonotic helminthes in the world that causing enormous economic and health losses. The aim of this study was to evaluate genotype of E. granulosus isolated from sheep using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and sequencing method in East Azerbaijan province, northwest of Iran. Nineteen sheep hydatid cyst samples were collected. Genomic DNA was extracted from protoscoleces using commercial DNA extraction kit. Mitochondrial cox1 region was amplified by polymerase chain reaction (PCR) and all isolates were sequenced. Afterward, sequences were analyzed for determination of genotypes by related software. G1 (94.73 %) and G3 (5.27 %) genotypes were identified from the isolates which out of 19 hydatid cysts, 17 samples were G1B, 1 sample G1D and the other one had G3 genotype. Results of this study indicate that common sheep strain (G1); especially G1B is the dominant subtype of E. granulosus in East Azerbaijan province.
KeywordsEchinococcus granulosus Cox1 Sheep Sequencing Iran
This work was supported fully by Tabriz Infectious and Tropical Diseases Research Center (Grant no: 92-01), Tabriz University of Medical Sciences, Tabriz, Iran. This is a report of a database from thesis of Nader Ghabouli Mehrabani entitled “Genotyping of Echinococcus granulosus isolates from human and herbivores using mitochondrial cytochrome oxidase 1(cox1) gene by sequencing method in Tabriz” registered in Tabriz University of Medical Sciences.
Conflict of interest
The authors declare that there is no conflict of interest.
- Busi M, Šnábel V, Varcasia A, Garippa G, Perrone V, De Liberato C, D’amelio S (2007) Genetic variation within and between G1 and G3 genotypes of Echinococcus granulosus in Italy revealed by multilocus DNA sequencing. Vet Parasitol 150:75–83. doi: 10.1016/j.vetpar.2007.09.003 CrossRefPubMedGoogle Scholar
- Ergin S, Saribas S, Yuksel P, Zengin K, Midilli K, Adas G, Arikan S, Aslan M, Uysal H, Caliskan R (2010) Genotypic characterisation of Echinococcus granulosus isolated from human in Turkey. Afr J Microbiol Res 4:551–555Google Scholar
- Ghabouli Mehrabani N, Kousha A, Khalili M, Mahami Oskouei M, Mohammadzadeh M, Alizadeh S, Maleksabet A, Hamidi F (2014) Hydatid cyst surgeries in patients referred to hospitals in East Azerbaijan province during 2009–2011. Iran J Parasitol 9(2):233–238Google Scholar
- Hajialilo E, Harandi MF, Sharbatkhori M, Mirhendi H, Rostami S (2012) Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol 86(3):263. doi: 10.1017/S0022149X11000320 CrossRefPubMedGoogle Scholar
- Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A, Ito A (2011) Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): an inference from nuclear protein-coding genes. Mol Phylogenet Evol 61:628–638. doi: 10.1016/j.ympev.2011.07.022 CrossRefPubMedGoogle Scholar
- M’rad S, Filisetti D, Oudni M, Mekki M, Belguith M, Nouri A, Sayadi T, Lahmar S, Candolfi E, Azaiez R (2005) Molecular evidence of ovine (G1) and camel (G6) strains of Echinococcus granulosus in Tunisia and putative role of cattle in human contamination. Vet Parasitol 129:267–272. doi: 10.1016/j.vetpar.2005.02.006 CrossRefPubMedGoogle Scholar
- Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M, Meamar A (2013) Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol 10:10Google Scholar
- Piccoli L, Bazzocchi C, Brunetti E, Mihailescu P, Bandi C, Mastalier B, Cordos I, Beuran M, Popa L, Meroni V (2013) Molecular characterization of Echinococcus granulosus in south-eastern Romania: evidence of G1–G3 and G6–G10 complexes in humans. Clin Microbiol Infect 19(6):578–582. doi: 10.1111/j.1469-0691.2012.03993.x CrossRefPubMedGoogle Scholar
- Rokni MB (2009) Echinococcosis/hydatidosis in Iran. Iran J Parasitol 4:1–16Google Scholar
- Sánchez E, Cáceres O, Náquira C, Garcia D, Patiño G, Silvia H, Volotão AC, Fernandes O (2010) Molecular characterization of Echinococcus granulosus from Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene. Mem Inst Oswaldo Cruz 105:806–810. doi: 10.1590/S0074-02762010000600013 CrossRefPubMedGoogle Scholar
- Schantz P (1995) Epidemiology and control of hydatid disease. In Thompson RCA, Lymbery AJ (eds) Echinococcus and hydatid disease, CAB International, Oxon, pp 233–331Google Scholar
- Schantz P (2003) Echinococcus species (agents of cystic, alveolar, and polycystic echinococcosis). Principles and practice of pediatric infectious diseases, 2nd edn. Churchill-Livingstone, New York, pp 1357–1361Google Scholar
- Vural G, Baca AU, Gauci CG, Bagci O (2008) Variability in the Echinococcus granulosus cytochrome C oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol 154:347–350. doi: 10.1016/j.vetpar.2008.03.020 CrossRefPubMedGoogle Scholar