Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran
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Echinococcus granulosus as an etiologic agent of hydatid cyst is one of the most important zoonotic helminthes in the world that causing enormous economic and health losses. The aim of this study was to evaluate genotype of E. granulosus isolated from sheep using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and sequencing method in East Azerbaijan province, northwest of Iran. Nineteen sheep hydatid cyst samples were collected. Genomic DNA was extracted from protoscoleces using commercial DNA extraction kit. Mitochondrial cox1 region was amplified by polymerase chain reaction (PCR) and all isolates were sequenced. Afterward, sequences were analyzed for determination of genotypes by related software. G1 (94.73 %) and G3 (5.27 %) genotypes were identified from the isolates which out of 19 hydatid cysts, 17 samples were G1B, 1 sample G1D and the other one had G3 genotype. Results of this study indicate that common sheep strain (G1); especially G1B is the dominant subtype of E. granulosus in East Azerbaijan province.
KeywordsEchinococcus granulosus Cox1 Sheep Sequencing Iran
This work was supported fully by Tabriz Infectious and Tropical Diseases Research Center (Grant no: 92-01), Tabriz University of Medical Sciences, Tabriz, Iran. This is a report of a database from thesis of Nader Ghabouli Mehrabani entitled “Genotyping of Echinococcus granulosus isolates from human and herbivores using mitochondrial cytochrome oxidase 1(cox1) gene by sequencing method in Tabriz” registered in Tabriz University of Medical Sciences.
Conflict of interest
The authors declare that there is no conflict of interest.
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