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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 785–790 | Cite as

Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran

  • Mahmoud Mahami Oskouei
  • Nader Ghabouli Mehrabani
  • Abolfazl Miahipour
  • Esmaeil Fallah
Original Article

Abstract

Echinococcus granulosus as an etiologic agent of hydatid cyst is one of the most important zoonotic helminthes in the world that causing enormous economic and health losses. The aim of this study was to evaluate genotype of E. granulosus isolated from sheep using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and sequencing method in East Azerbaijan province, northwest of Iran. Nineteen sheep hydatid cyst samples were collected. Genomic DNA was extracted from protoscoleces using commercial DNA extraction kit. Mitochondrial cox1 region was amplified by polymerase chain reaction (PCR) and all isolates were sequenced. Afterward, sequences were analyzed for determination of genotypes by related software. G1 (94.73 %) and G3 (5.27 %) genotypes were identified from the isolates which out of 19 hydatid cysts, 17 samples were G1B, 1 sample G1D and the other one had G3 genotype. Results of this study indicate that common sheep strain (G1); especially G1B is the dominant subtype of E. granulosus in East Azerbaijan province.

Keywords

Echinococcus granulosus Cox1 Sheep Sequencing Iran 

Notes

Acknowledgments

This work was supported fully by Tabriz Infectious and Tropical Diseases Research Center (Grant no: 92-01), Tabriz University of Medical Sciences, Tabriz, Iran. This is a report of a database from thesis of Nader Ghabouli Mehrabani entitled “Genotyping of Echinococcus granulosus isolates from human and herbivores using mitochondrial cytochrome oxidase 1(cox1) gene by sequencing method in Tabriz” registered in Tabriz University of Medical Sciences.

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Mahmoud Mahami Oskouei
    • 1
    • 2
  • Nader Ghabouli Mehrabani
    • 1
    • 2
  • Abolfazl Miahipour
    • 1
    • 2
  • Esmaeil Fallah
    • 2
  1. 1.Tabriz Infectious and Tropical Diseases Research CenterTabriz University of Medical SciencesTabrizIran
  2. 2.Department of Parasitology, Faculty of MedicineTabriz University of Medical SciencesTabrizIran

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