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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 730–734 | Cite as

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

  • Shawky M Aboelhadid
  • Marwa Thabet
  • Dayhoum El-Basel
  • Ragaa Taha
Original Article
  • 97 Downloads

Abstract

The present study aims to detect the digenetic larvae infections in Bulinus truncatus and Biomphalaria alexandrina snails and also PCR detection of Schistosoma mansoni infection. The snails were collected from different branches of Yousef canal and their derivatives in El Fayoum Governorate. The snails were investigated for infection through induction of cercarial shedding by exposure to light and crushing of the snails. The shed cercariae were S. mansoni, Pharyngeate longifurcate type I and Pharyngeate longifurcate type II from B. alexandrina, while that found in B. truncatus were Schitosoma haematobium and Xiphidiocercaria species cercariae. The seasonal prevalence of infection was discussed. Polymerase chain reaction was used for the detection of S. mansoni in the DNA from field collected infected and non infected snails. The results of PCR showed that the pool of B. alexandrina snails which shed S. mansoni cercariae in the laboratory, gave positive reaction in the samples. Pooled samples of field collected B. alexandrina that showed negative microscopic shedding of cercariae gave negative and positive PCR in a consecutive manner. Accordingly, a latent infection in the snail (negative microscopic) could be detected by using PCR.

Keywords

Biomphalaria alexandrina Bulinus truncatus Schistosoma mansoni Cercariae PCR 

Notes

Acknowledgments

The authors appreciated to efforts of Dr. Abel Azim Abdel Baky for helping in this work.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Shawky M Aboelhadid
    • 1
  • Marwa Thabet
    • 2
  • Dayhoum El-Basel
    • 2
  • Ragaa Taha
    • 2
  1. 1.Parasitology Department, Faculty of Veterinary MedicineBeni Suef UniversityBeni SuefEgypt
  2. 2.Zoology Department, Faculty of ScienceEl Fayoum UniversityEl FaiyumEgypt

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