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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 694–698 | Cite as

Fenvalerate resistance status in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) from Punjab, India

  • Jyoti
  • Abhijit Nandi
  • Harkirat Singh
  • N. K. Singh
  • S. S. Rath
Original Article

Abstract

Larval packet test was used for evaluating the resistance levels in Rhipicephalus (Boophilus) microplus collected from different districts of central plain zone of Punjab state, India against fenvalerate. The regression graphs of probit mortality of larvae plotted against log values of progressively increasing concentrations of fenvalerate were utilized for the estimation of lethal concentration for 50 % (LC50) and 95 % (LC95) values against various field isolates of R. (B.) microplus. The slope of mortality (95 % confidence levels) varied from 0.730 ± 0.097 (0.419–1.043) to 1.455 ± 0.281 (0.558–2.352) and the value of R2 varied from 0.881 to 0.997. From the regression equation the values of LC50 and LC95 were recorded in range of 184.39–1,338.01 and 3,253.33–112,706.26 ppm, respectively. Among the various tick isolates resistance factors in range of 1.56–54.34 were determined and all field isolates studied were found resistant against fenvalerate. Two field isolates (Jalandhar and Ludhiana) showed level I resistance; three (Patiala, Fatehgarh Sahib and Amritsar) showed level II and Kapurthala isolate showed level IV resistance. The data generated on fenvalerate resistant status will help in judicious use of the drug and formulation of effective tick control strategy for the region.

Keywords

Acaricide resistance Fenvalerate LPT Punjab Rhipicephalus (Boophilus) microplus 

Notes

Acknowledgments

The authors are grateful to Department of Science and Technology, New Delhi for funding through Women Scientist Scheme (WOS-A) Project No. SR/WOS-A/LS-493/2011 to the senior author. Sincere thanks are also due to the Director of Research, GADVASU, Ludhiana and Dr S Ghosh (Principal Scientist) Division of Parasitology, Indian Veterinary Research Institute, Izatnagar India for providing necessary help.

Conflict of interest

Nil.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Jyoti
    • 1
  • Abhijit Nandi
    • 1
  • Harkirat Singh
    • 1
  • N. K. Singh
    • 1
  • S. S. Rath
    • 2
  1. 1.Department of Veterinary Parasitology, College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia
  2. 2.ADRC, College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

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