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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 667–673 | Cite as

In vitro studies on medicinal plants used against bacterial diabetic foot ulcer (BDFU) and urinary tract infected (UTI) causing pathogens

  • S. Subbu lakshmi
  • G. Chelladurai
  • B. Suresh
Original Article

Abstract

The pus samples from diabetic foot ulcer patients and urine samples from urinary tract infected patients were collected and inoculated in nutrient agar plates. The colonies showing different morphologies were streaked on selective agar plates. The antibacterial assay of selected commercial antibiotics was tested against the foot ulcer and urinary tract isolates. The result revealed that most of the organisms were found to be resistant against the antibiotics. Screening of antibacterial activity of selected plants, methanol extracts of plants were prepared and tested against foot ulcer pathogens. Among the plants used, the methanolic extract Tragia involucrata was very effective against the foot ulcer pathogens and to separate the compounds present in the methanolic extract of T. involucrata, when it was subjected to column chromatography. The fractions obtained were further checked for their antibacterial property and fraction 1 which inhibited the pathogens, were subjected to thin layer chromatography and the structure of the particular phytochemical compound was elucidated by NMR study. The spices were tested for their antibacterial property against the urinary tract pathogens. Among the spices tested; Allium sativum inhibited the growth of the pathogens isolated from urinary tract infection. It can be concluded that the plants extract can be used to discover natural products that may serve as lead for the development of new pharmaceuticals addressing the major therapeutic needs.

Keywords

Medicinal plants and spices Antibacterial activity Chromatography NMR 

Notes

Acknowledgments

We are great full thanks to Principal and Director of Kamaraj College, for providing lab facilities and for the encouragement throughout the study.

Conflict of interest

All contributing authors declare no conflicts of interest.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  1. 1.Department of MicrobiologyKamaraj CollegeTuticorinIndia
  2. 2.Department of ZoologyKamaraj CollegeTuticorinIndia
  3. 3.Department of ChemistryKamaraj CollegeTuticorinIndia

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