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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 652–655 | Cite as

Polypeptide profiles of South Indian isolate of Trypanosoma evansi

  • S. Sivajothi
  • V. C. Rayulu
  • B. V. Bhaskar Reddy
  • P. Malakondaiah
  • D. Sreenivasulu
  • B. Sudhakara Reddy
Original Article
  • 75 Downloads

Abstract

The field isolates of Trypanosoma evansi was collected from the infected cattle and it was propagated in rats. Trypanosoma evansi parasites were separated from the blood of infected rats by using diethylaminoethyl cellulose column chromatography. Whole cell lysate antigen (WCL) was prepared from purified trypanosomes by ultrasonication and centrifugation. The prepared WCL antigen was further purified by 50 % ammonium sulphate precipitation. Protein concentration of WCL antigen of T. evansi was 60 mg/ml. Protein concentration was adjusted to 1.0 mg/ml in PBS, pH 8.0 and stored at −200 C.   Polypeptide profiles of WCL antigen of T. evansi was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A total of eight polypeptide bands of the size ranging from 25 to 85 kDa in WCL antigen of T. evansi were obtained. Five prominent bands with molecular weight of 74, 60, 53, 42 and 37 kDa and three light bands with molecular weight of 85, 34 and 25 kDa were observed.

Keywords

Cattle Polypeptide profiles SDS-PAGE Trypanosoma evansi Whole cell lysate antigen 

Notes

Acknowledgments

The authors acknowledge the authorities of Sri Venkateswara Veterinary University, Tirupati for providing facilities to carry out this research.

References

  1. Davison HC, Thrusfield MV, Muharsini S, Husein A, Partoutomo S, Rae PF, Masake R, Luckins AG (1999) Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia. Epidemiol Infect 123:149–155CrossRefPubMedPubMedCentralGoogle Scholar
  2. Giardina S, Paganico G, Urbani G, Rossi M (2003) A biochemical and immunological comparative study on Trypanosoma equiperdum and Trypanosoma evansi. Vet Res Commun 27:289–300CrossRefPubMedGoogle Scholar
  3. Jithendran KP, Rao JR (2001) Antigenic characterization of blood stream variant of an Indian isolates of Trypanosoma evansi from buffaloes. J Vet Parasitol 15:17–23Google Scholar
  4. Kashiwazaki Y et al (1998) Haemoparasite infections in newly introduced dairy cattle in Loei Province, Thailand: Trypanosoma evansi antigen levels by ELISA referring to abortion. Vet Parasitol 80:99–109CrossRefPubMedGoogle Scholar
  5. Laemmli UK (1970) Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature 227:680–685CrossRefPubMedPubMedCentralGoogle Scholar
  6. Laha R, Sasmal NK, Bandyopadhyay S (2008) Comparative polypeptide profiles of whole cell lysate antigens of Trypanosoma evansi isolated from three different hosts of eastern India. J Protozool Res 18:11–16Google Scholar
  7. O.I.E. (2008) Trypanosoma evansi infections (including surra) In: OIE Terrestrial manual 2008. Office International des Epizooties World Health Organization for animal health, Paris. pp: 352–360Google Scholar
  8. Pareek RK, Pathak KML, Kumar S, Garg R, Kapoor M (1999) Polypeptide profile of Trypanosoma evansi isolates of camel and cattle origin. J Vet Parasitology 13(1):53–54Google Scholar
  9. Pathak KML, Arora JK, Kapoor M (1993) Camel trypanosomosis in Rajasthan, India. Vet Parasitol 49:319–323CrossRefPubMedGoogle Scholar
  10. Rayulu VC (2008) Characterization of surface antigens of Trypanosoma evansi by monoclonal antibodies and development of suitable assays. J Vet Parasitol 22(1):89Google Scholar
  11. Singh V, Singh A, Chhabra MB (1994) Polypeptide profile of whole cell lysate of Trypanosoma evansi stocks from northern India. Indian J Anim Sci 64:14–17Google Scholar
  12. Sivajothi S, Rayulu VC, Reddy BS (2012) Development of slide enzyme linked immunosorbent assay (SELISA) for detection of Trypanosoma evansi infection in bovines. J Adv Vet Res 2:15–17Google Scholar
  13. Sivajothi S, Rayulu VC, Reddy BS (2013a) Haematological and biochemical changes in experimental Trypanosoma evansi infection in rabbits. J parasit dis. doi: 10.1007/s12639-013-0321-6 CrossRefPubMedPubMedCentralGoogle Scholar
  14. Sivajothi S, Rayulu VC, Malakondaiah P, Sreenivasulu D (2013b) Colloidal dye immunobinding assay for detection of Trypanosoma evansi antibodies in animals. Intern J Livest Res 3(3):48–56CrossRefGoogle Scholar
  15. Sivajothi S, Rayulu VC, Sujatha K, Reddy BS (2014a) Study of histopathological changes in experimental Trypanosoma evansi infected rats. Proc Zool Soc. doi: 10.1007/s12595-014-0104-9 CrossRefGoogle Scholar
  16. Sivajothi S, Reddy BS, Kumari KN, Rayulu VC (2014b) Haematological changes in Trypanosoma evansi infected cattle. Intern J Sci World 2(1):27–30Google Scholar
  17. Sivajothi S, Rayulu VC, Malakondaiah P, Sreenivasulu D (2014c) Diagnosis of Trypanosoma evansi in bovines by indirect ELISA. J Parasit Dis. doi: 10.1007/s12639-014-0465-z CrossRefPubMedPubMedCentralGoogle Scholar
  18. Uzcanga G, Mendoza M, Aso PM, Bubis J (2002) Purification of a 64 kDa antigen from Trypanosoma evansi that exhibits cross-reactivity with Trypanosoma vivax. Parasitology 124(3):287–299CrossRefPubMedGoogle Scholar
  19. Zheng RJ, Sheng J, Qui QP, Wang HA (1990) Comparison of protein components and antigenicities of various strains of Trypanosoma evansi from Zhejiasg, Yunan and Anhui provinces. Chinese J Vet Sci Technol 2:7–9Google Scholar

Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • S. Sivajothi
    • 1
  • V. C. Rayulu
    • 1
  • B. V. Bhaskar Reddy
    • 2
  • P. Malakondaiah
    • 3
  • D. Sreenivasulu
    • 4
  • B. Sudhakara Reddy
    • 5
  1. 1.Department of Veterinary Parasitology, C.V.Sc.S.V.V.U.ProddaturIndia
  2. 2.RARSANGRAUTirupatiIndia
  3. 3.Department of Veterinary Parasitology, C.V.Sc.S.V.V.U.TirupathiIndia
  4. 4.C.V.Sc.S.V.V.U.TirupatiIndia
  5. 5.Teaching Veterinary Clinical Complex (Veterinary Medicine), C.V.Sc.S.V.V.U.ProddaturIndia

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