Utilizing ribosomal DNA gene marker regions to characterize the metacercariae (Trematoda: Digenea) parasitizing piscine intermediate hosts in Manipur, Northeast India
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Freshwater fishes in Manipur, Northeast India frequently harbour several types of metacercariae, which based on morphological criteria were identified as Clinostomoides brieni, Euclinostomum heterostomum (Clinostomidae) and Polylekithum sp. (Allocreadiidae). Molecular techniques utilizing PCR amplification of rDNA regions of larger subunit (LSU or 28S), smaller subunit (SSU or 18S) and inter transcribed spacers (ITS1, 2) were used for molecular characterization of these types. Sequences generated from the metacercariae were compared with their related sequences available in public databases; an analysis of the identity matrices and phylogenetic trees constructed was also carried out, which confirmed their identification. Similarly, the sequences generated from Polylekithum sp. were found to be highly similar to the species of the same genus. The rDNA ITS2 secondary structure provided additional confirmation of the robustness of the molecular marker as a tool for taxon-specific characterization.
KeywordsMolecular rDNA ITS2 28S 18S Metacercaria Fish hosts Clinostomidae Allocreadiidae India
VDA acknowledges the award of Research Fellowship for meritorious students by University Grants Commission (UGC) and Senior Research Fellowship by Council of Scientific & Industrial Research (CSIR), Government of India.
- Gibson DI, Jones A, Bray RA (2002) Keys to the Trematoda, vol 1. Natural History Museum, CABIGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 4:95–98Google Scholar
- Jaiswal GP (1957) Studies on the trematode parasites of fishes and birds found in Hyderabad State. Zool Jahrb Abt Syst Part I-IV 85(12):1–72Google Scholar
- Jones A, Bray RA, Gibson DI (2005) Keys to the Trematoda, vol 2. Natural History Museum, CABIGoogle Scholar
- Kanev I, Radev V, Fried B (2002) Family Clinostomidae Lühe, 1901. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CABI Publishing and the Natural History Museum, London, pp 113–120Google Scholar
- Tkach VV, Pawlowski J, Sharpilo VP (2000) Molecular and morphological differentiation between species of the Plagiorchis vespertilionis group (Digenea, Plagiorchiidae) occurring in European bats, with a re-description of P. vespertilionis (Müller, 1780). Syst Parasitol 47:9–22CrossRefPubMedGoogle Scholar
- Verma SC (1936) Notes on trematode parasites of Indian birds. Part. I. Allahabad Univ Studies 12:147–188Google Scholar
- Vidyarthi RD (1938) New avian trematodes (family: Diplostomidae) from Indian birds. Proc Nat Acad Sci India 8(3):76–84Google Scholar
- Yamaguti S (1971) Synopsis of the digenetic trematodes of vertebrates, vol 1. Keigaku Publishers, Japan, p 1074Google Scholar