Advertisement

Journal of Parasitic Diseases

, Volume 40, Issue 1, pp 52–56 | Cite as

Sero-epidemiological value of some hydatid cyst antigen in diagnosis of human cystic echinococcosis

  • Mohey Abdelhafez Hassanain
  • Raafat Mohamed Shaapan
  • Fathia Abdelrazik M. Khalil
Original Article

Abstract

Cystic echinococcosis (CE) is a severe zoonotic disease which affects both human and animals. The disease has a considerable economic and social impact, because it has numerous complications leading to important disabilities and even death. CE is a widespread chronic endemic helminthic disease caused by infection with metacestodes of tapeworm Echinococcus granulosus. This study was conducted to diagnosis human CE by hydatid cyst antigens from camels and sheep. Hydatid fluid and protoscoleces crude antigens corresponding to camel and sheep were resolute by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and the protein bands of different antigens were exposed to infected patients serum CE through western blot (WB) assay. The camel hydatid fluid antigen revealed five polypeptide bands of 18–98.8 kDa by SDS-PAGE while sheep hydatid fluid antigen revealed four polypeptide bands of 20–100 kDa. Immune reactive bands were obtained through WB ranged from 25 to 125 kDa. The study showed prominent immune reactive bands of 92, 52.2 and 35.7 kDa which may helpful in diagnosis of human CE.

Keywords

Cystic echinococcosis (CE) Human Camel Sheep SDS-PAGE Western blot (WB) 

References

  1. Abdel-Rahman EH, Abdel-Megeed KN, Abuel-Ezz NM (2003) Cross-reaction: a common trait among helminthes. J Egypt Soc Parasitol 33:457–471PubMedGoogle Scholar
  2. Ciobanca PT, Junie ML (2011) Diagnosis confirmation of human cystic echinococcosis by imagistic methods and immunoserological determinations. Sci Parasitol 12(3):151–161Google Scholar
  3. Constantea N, Ciobanca P (2007) Studiul clinic pentru imunatatirea metodelor de diagnostic de laborator si profilaxia chistului hidatic. Rev Rom Parasitol 17:48–49Google Scholar
  4. Derbala AA, Zayed AA (1997) Prevalence, fertility and viability of cysticercosis and hydatidosis infection in some domestic animals. J. Union Arab Biol Cairo 7A:109–123Google Scholar
  5. Eckert J, Deplazes P (2004) Biological, epidemiological and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 17(1):107–135CrossRefPubMedPubMedCentralGoogle Scholar
  6. El Zayyat EA, Ramzy RM, Abdel-Baki MH, Fahmi IA, Rifaat M, Helmy H, Abdel Hameed DM (1999) Human cystic echinococcosis: diagnostic value of different antigenic fractions of hydatid cyst fluid with different specific immunoglobulin G subclasses by enzyme linked immuno-electrotransfer blot. J Egypt Soc Parasitol 29:817–830PubMedGoogle Scholar
  7. Gasser RB, Lightowlers MW, Obendorf DL, Jenkins DJ, Rickard MD (1988) Evaluation of a serological test system for the diagnosis of natural Echinococcus granulosus infection in dogs using E. granulosus protoscolex and oncosphere antigens. Aust Vet J 65:369–373CrossRefPubMedGoogle Scholar
  8. Heath DD, Lawrence SB, Yong WK (1992) Echinococcus granulosus in sheep: transfer from ewe to lamb of Arc5 antibodies and onchospher-killing activity, but not protein. Int J Parasitol 22:1017–1021CrossRefPubMedGoogle Scholar
  9. Hernandez A, Nieto A (1994) Induction of protective immunity against murine secondary hydatidosis. Parasite Immunol 16:527–544CrossRefGoogle Scholar
  10. Himonas C, Antoniadou S, Papadopoulos E (1994) Hydatidosis of food animals in Greece (prevalence of cysts containing viable protoscoleces). J Helminthol 68:311–313CrossRefPubMedGoogle Scholar
  11. Huang M, Zheng H (2012) Clinical and demographic characteristics of patients with urinary tract hydatid disease. PLoS One 7(11):1–6Google Scholar
  12. Ito A, Urbani C, Jiamin Q, Vuitton DA, Dongchuan Q, Heath DD, Craig PS, Zheng F, Schantz PM (2003) Control of echinococcosis and cysticercosis: a public health challenge to international cooperation in China. Acta Trop 86:3–17CrossRefPubMedGoogle Scholar
  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMedGoogle Scholar
  14. Logar J, Šoba B, Lejko-Zupanc T, Kotar T (2008) Serological evidence for human cystic echinococcosis in Slovenia. BMC Infect Dis 8:1–4CrossRefGoogle Scholar
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:262–275Google Scholar
  16. Mahmoud MS, Derbala AA, El-Massry AA, Maarouf OA (2008) Sero-diagnostic potency of hydatid fluid and protoscoleces partially purified fractions of both camel and equine origin. Global Veterinaria 2(3):99–103Google Scholar
  17. Mandal S, Mandal MD (2012) Human cystic echinococcosis: epidemiologic, zoonotic, clinical, diagnostic and therapeutic aspects. Asian Pac J Trop Med 5(4):253–260CrossRefPubMedGoogle Scholar
  18. Nasrieh MA, Abdel-Hafez SK (2004) Echinococcus granulosus in Jordan: assessment of various antigenic preparations for use in the serodiagnosis of surgically confirmed cases using enzyme immuno assays and the indirect haemagglutination test. Diagn Microbiol Infect Dis 48(2):117–123CrossRefPubMedGoogle Scholar
  19. Rausch RL (1995) Life cycle patterns and geographic distribution of Echinococcus species. In: Thompson RCA, Lymbery AJ (eds) Echinococcus and hydatid disease. CAB International, Wallingford, pp 89–134Google Scholar
  20. Sarkari B, Sadjjadi SM, Abidi H, Izadpanah Kazemian S, Rafati A (2007) Application of western blotting using native antigen B for serodiagnosis of human echinococcosis. Iranian J Parasitol 2(3):7–12Google Scholar
  21. Schantz PM (1991) Parasitic zoonoses in perspective. Int J Parasitol 21(2):161–170CrossRefPubMedGoogle Scholar
  22. Siracusano A, Margutti P, Delunardo F, Profumo E, Rigano R (2008) Molecular cross-talk in host-parasite relationships: the intriguing immunomodulatory role of Echinococcus antigen B in cystic echinococcosis. Int J Parasitol 38:1371–1376CrossRefPubMedGoogle Scholar
  23. Tawfeek GM, Elwakil HS, Awad NS, El-Hoseiny L, Thabet HS, Sarhan RM, Darweesh SK, Anwar WA (2009) Genetic variability of antigen B among Echinococcus granulosus Egyptian isolates. Korean J Parasitol 47(3):259–264CrossRefPubMedPubMedCentralGoogle Scholar
  24. Tawfeek GM, Elwakil HS, El-Hoseiny L, Thabet HS, Sarhan RM, Awad NS, Anwar WA (2011) Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit (12 kDa), assessed by ELISA, in the diagnosis of human cystic echinococcosis. Parasitol Res 108(2):371–376CrossRefPubMedGoogle Scholar
  25. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 176:4350–4354CrossRefGoogle Scholar
  26. Wray W, Boulikas T, Wray VP, Hancock R (1981) Silver staining of proteins in polyacrylamide gels. Anal Biochem 118:197–203CrossRefPubMedGoogle Scholar
  27. Youssef MR, Hosseini SH, Hassan MT (2010) Evaluation and comparison response in laboratory model to low antigen of fluid and protoscolex in hydatid cyst. Global Veterinaria 4(6):622–625Google Scholar
  28. Zhang Y, Bart JM, Giraudoux P, Craig P, Vuitton D, Wen H (2006) Morphological and molecular characteristics of Echinococcus multilocularis and Echinococcus granulosus mixed infection in a dog from Xinjiang. China Vet Parasitol 139(1–3):244–248CrossRefPubMedGoogle Scholar

Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Mohey Abdelhafez Hassanain
    • 1
  • Raafat Mohamed Shaapan
    • 1
  • Fathia Abdelrazik M. Khalil
    • 2
  1. 1.Department of Zoonotic DiseasesNational Research CenterGizaEgypt
  2. 2.Department of Parasitology and Animal DiseasesNational Research CenterGizaEgypt

Personalised recommendations