Journal of Parasitic Diseases

, Volume 39, Issue 4, pp 770–772 | Cite as

First identification of Sarcocystis hominis in Iranian traditional hamburger

  • M. Moghaddam Ahmadi
  • B. Hajimohammadi
  • G. Eslami
  • A. Oryan
  • S. A. Yasini Ardakani
  • A. Zohourtabar
  • S. Zare
Original Article

Abstract

Zoonotic concerns of cattle sarcocystosis are of importance, because humans are the final host for Sarcocystis hominis. Therefore the meat products containing beef may encompass sarcocysts which endanger food safety. In this study, we described the first report of molecular identification of S. hominis in Iranian traditional hamburgers using PCR-RFLP. Throughout a pilot research that was carried out to setup a molecular approach to identify the Sarcocystis spp., using PCR-RFLP, a sample of raw Iranian traditional hamburger was purchased from a street food seller located in Yazd, central Iran in May 2013. DNA extraction was done, by salting out method; briefly, the sample was lysed with NET buffer. The DNA purification and precipitation was then performed. Amplicon and digestion results were analyzed, using gel agarose electrophoresis. The results showed a PCR product with 926 bp in length after amplification and 376 and 550 bp in length after digestion. This product was identified as S. hominis. To the best of our knowledge, this is the first report of S. hominis infection in Iranian hamburger.

Keywords

S. hominis PCR-RFLP Iranian hamburger 

Notes

Acknowledgments

This study was conducted at the Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. We thank the authorities of Shahid Sadoughi University of Medical Sciences for their financial support and assistance.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • M. Moghaddam Ahmadi
    • 1
  • B. Hajimohammadi
    • 1
  • G. Eslami
    • 2
  • A. Oryan
    • 3
  • S. A. Yasini Ardakani
    • 4
  • A. Zohourtabar
    • 1
  • S. Zare
    • 5
  1. 1.Department of Food Hygiene and Safety, Faculty of HealthShahid Sadoughi University of Medical SciencesYazdIran
  2. 2.Department of Parasitology and Mycology, Faculty of MedicineShahid Sadoughi University of Medical SciencesYazdIran
  3. 3.Department of Pathology, School of Veterinary MedicineShiraz UniversityShirazIran
  4. 4.Department of Food Science and Technology, Science and Research BranchIslamic Azad UniversityYazdIran
  5. 5.Yazd Health OfficeYazdIran

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