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Journal of Parasitic Diseases

, Volume 39, Issue 3, pp 385–392 | Cite as

RETRACTED ARTICLE: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India

  • S. BalakrishnanEmail author
  • K. Indira
  • M. Srinivasan
Original Article

Abstract

Samples collected from the mangroves of Vellar estuary yielded a mosquitocidal bacterium, whose secondary metabolites exhibited mosquito larvicidal and pupicidal activity. The bacterium was isolated using standard microbiological methods and identified using classical biochemical tests. The mosquitocidal bacterium was identified as Bacillus subtilis, Bacillus thuringiensis, Bacillus sphaericus and Bacillus cereus. Mosquitocidal metabolite(s) was separated from the culture supernatant of the bacterium and its efficacy was against the larval and pupal stages of two different species of mosquitoes and determined in terms of LC50 and LC90. Mosquito larvicidal activity in terms of LC50 against Anopheleus stephensi and Aedes aegypti was 4.374 and 7.406 μl/ml and its pupicidal activity was 4.928 and 9.865 μl/ml, respectively. The present study proved that the mosquitocidal properties of the Bacillus species isolated from mangroves of Vellar estuary was evaluated as target species of mosquito vectors. This is an ideal eco-friendly approach for the vector control programs.

Keywords

Bacillus species Culture supernatant Mosquito pupicidal Mangroves Vellar estuary 

Notes

Acknowledgments

We thank the authorities of Annamalai University for providing the necessary facilities and the first author thanks to the INCOIS-SATCORE Project (G4/515/2008), Ministry of Earth Sciences (Government of India) and second author thanks to the DST-PURSE Programme, Department of Science and Technology (Government of India) for financial support during the period of study. We also thank the anonymous referees for the valuable comments, which greatly improved our manuscript.

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Copyright information

© Indian Society for Parasitology 2013

Authors and Affiliations

  1. 1.Centre of Advanced Study in Marine Biology, Faculty of Marine SciencesAnnamalai UniversityParangipettaiIndia

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