Journal of Parasitic Diseases

, Volume 39, Issue 2, pp 292–297

Immune responses against rHaa86 in cross-bred cattle

Original Article


Tick vaccines are important component of integrated pest management for sustainable control of tick and tick born diseases. Immune responses against rHaa86 (homologue of Bm86) recombinant Hyalomma tick antigen were determined in experimental crossbred calves. The humoral antibody responses of the calves were measured against rHaa86 in an optimized ELISA format. The expression of the interferon gamma (IFN-γ), was also evaluated in the culture supernatant of blood culture from blood samples of the experimental calves. The expression patterns were studied after stimulating the blood cells in vitro with rHaa86 antigen and subsequently optical density was measured against IFN-γ. The results were expressed as stimulation indices. All the rHaa86 immunized animal showed strong humoral antibody response just after 1st vaccination and reach to pick after 2nd booster and thereafter maintained up to days 120 from post primary immunization. The humoral antibody response was dominated by IgG1 against IgG2 throughout the period of antibody monitoring. The standard graph of bovine recombinant IFN-γ was plotted which showed a significant difference in SI and OD value up to 200 pg/ml. The lowest detectable value of IFN-γ was 20 pg/ml and SI at this level is 1.16 which is greater than maximum SI calculated from individual calf. The IFN-γ response never reached at significant level and the IgG1 response was dominated over IgG2 response throughout the period of experiment. Since IgG2 and IFN-γ are interlinked, the present study established the Th2 response as a possible mode of mechanism of conferring antibody mediated protection against challenged ticks.


Hyalomma rHaa86 IgG1 IgG2 IFN-γ 


  1. Allen JR (1989) Immunology, immunopathology, and immunoprophylaxis of tick and mite infestations. In: Soulsby EJL (ed) Immune responses in parasitic infections: immunology, immunopathology and immunoprophylaxis, vol 4. CRC Press, Boca RatonGoogle Scholar
  2. Azhahianambi P, Ray DD, Chaudhuri P, Gupta R, Ghosh S (2009a) Vaccine efficacy of Bm86 ortholog of hyalomma anatolicum anatolicum rHaa86 expressed in prokaryotic expression system. J Parasitol res. doi:10.1155/2009/165812 PubMedCentralPubMedGoogle Scholar
  3. Azhahianambi P, Suryanarayana VVS, Ghosh S (2009b) Cloning expression and immunoprotective efficacy of rHaa86, the homologue of the Bm86 tick vaccine antigen, from Hyalomma anatolicum anatolicum. Parasite Immunol 31:111–122CrossRefPubMedGoogle Scholar
  4. Clery D, Torgerson P, Mulcahy G (1996) Immune responses of chronically infected adult cattle to Fasciola hepatica. Vet Parasitol 62:71–76CrossRefPubMedGoogle Scholar
  5. Cobon GS, Willadsen P (1990) Vaccine to prevent cattle tick infestations in new generation vaccine. Woodrow, Levine and Marcel, New York 901Google Scholar
  6. Estes DM, Brown WC (2002) Type 1 and type 2 responses in regulation of Ig isotype expression in cattle. Vet Immunol Immunopathol 90:1–10CrossRefPubMedGoogle Scholar
  7. Estes DM, Closser NM, Allen GK (1994) IFN-gamma stimulates IgG2 production from bovine B cells co-stimulated with anti-mu and mitogen. Cell Immunol 154:287–295CrossRefPubMedGoogle Scholar
  8. Estes DM, Hirano A, Heussler VT, Dobbelaere DA, Brown WC (1995) Expression and biological activities of bovine interleukin-4: effects of recombinant bovine interleukin-4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cell Immunol 163:268–273CrossRefPubMedGoogle Scholar
  9. Ghosh S, Singh NK, Das G (2005) Assessment of duration of immunity in crossbred cattle immunized with glycoproteins isolated from Hyalomma anatolicum anatolicum and Boophilus microplus. Parasitol Res 95:319–326CrossRefPubMedGoogle Scholar
  10. Hasvold HJ, Valheim M, Berntsen G, Storset AK (2002) In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium avium subsp. paratuberculosis. Vet Immunol Immunopathol 90:79–89CrossRefPubMedGoogle Scholar
  11. Jeyabal L, Azhahianambi P, Susitha K, Ray DD, Chaudhuri P, Vanlahmuaka, Ghosh S (2010) Efficacy of rHaa86, an orthologue of Bm86, against challenge infestations of Hyalomma anatolicum anatolicum. Transbound Emerg Dis 57:96–102CrossRefPubMedGoogle Scholar
  12. Kemp DH, Agbede RIS, Johnston LAY, Gough JM (1986) Immunisation of cattle against Boophilus microplus using extract derived from adult female ticks: feeding and survival of the parasite on the vaccinated cattle. Int J Parasitol 16:115–119CrossRefPubMedGoogle Scholar
  13. Kumar B, Azhahianambi P, Ray DD, Chaudhuri P, de la Fuente J, Kumar R, Ghosh S (2012) Comparative efficacy of rHaa86 and rBm86 against Hyalomma anatolicum anatolicum and Rhipicephalus (Boophilus) microplus. Immuno Parasitol 34:297–301CrossRefGoogle Scholar
  14. Laman JD, Claassen E, Noelle RJ (1996) Functions of CD40 and its ligand, GP39 (CD40L). Crit Rev Immunol 16:59–65CrossRefPubMedGoogle Scholar
  15. Lambertz C, Chongkasikit N, Jittapalapong S, Gauly M (2012) Immune response of Bos indicus cattle against the anti-tick antigen Bm91 derived from local Rhipicephalus (Boophilus) microplus ticks and its effect on tick reproduction under natural infestation. J Parasitol Res. Article (ID) 907607. doi:10.1155/2012/907607
  16. Mond JJ, Lees A, Snapper CM (1995) T cell independent antigens type 2. Annu Rev Immunol 13:655–661CrossRefPubMedGoogle Scholar
  17. Odongo D, Kamau L, Skilton R, Mwaura S, Nitsch C, Musoke A, Taracha E, Daubenberger C, Bishop R (2007) Vaccination of cattle with TickGARD induce corss-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus. Vaccine 25:1287–1296CrossRefPubMedGoogle Scholar
  18. Rand KN, Moore T, Sriskantha A, Spring K, Tellam R, Willadsen P, Cobon SG (1989) Cloning and expression of a protective antigen from the cattle tick Boophilus microplus. Proc National Acad Sci 86:9657–9661CrossRefGoogle Scholar
  19. Rodriguez M, Rubiera R, Penichet M, Montesions R, Cremata J, Falcon V, Sanchez G, Bringas R, Cordoves C, Valdes M, Lleonart R, Herrera L, de la Fuente J (1994) High level expression of the Boophilus microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle. J Biotecnol 33:135–146CrossRefGoogle Scholar
  20. Singh NK, Ghosh S (2003) Experimental immunization of crossbred cattle with glycoproteins isolated from the larvae of Hyalomma anatolicum anatolicum. Exp Appl Acarol 31:297–314CrossRefPubMedGoogle Scholar
  21. Snapper CM, Waegell W, Beernink H, Dasch JR (1993) Transforming growth factor-beta 1 is required for secretion of IgG of all subclasses by LPS-activated murine B cells in vitro. J Immunol 151:4625–4636PubMedGoogle Scholar
  22. Talwar GP, Gupta SK (1992) A hand book of practical and clinical immunology, vol 1. CBS Publishers and distributors, Delhi, pp 219–232Google Scholar
  23. Weynants V, Godfroid J, Limbourg B, Saegerman C, Letesson JJ (1995) Specific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production. J Clin Microbiol 33:706–712PubMedCentralPubMedGoogle Scholar
  24. Willadsen P, McKenna RV, Riding GA (1988) Isolation from the cattle tick Boophilus microplus, of antigenic material capable of eliciting a protective immunological response in the bovine host. Int J Parasitol 18:183–188CrossRefPubMedGoogle Scholar
  25. Willadsen P, Riding G, Mckenna RV, Kemp DH, Tellam RL, Nielsen JN, Lahnstein J, Cobon GS, Gough JM (1989) Immunological control of the parasitic arthropod. Identification of a protective antigen from Boophilus microplus. J Immunol 143:1346–1351PubMedGoogle Scholar

Copyright information

© Indian Society for Parasitology 2013

Authors and Affiliations

  1. 1.Entomology Laboratory, Division of ParasitologyIndian Veterinary Research InstituteIzatnagarIndia
  2. 2.Department of Veterinary Parasitology, College of Veterinary Science and Animal HusbandryJunagadh Agricultural UniversityJunagadhIndia

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