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Journal of Parasitic Diseases

, Volume 39, Issue 2, pp 162–167 | Cite as

In vitro protoscolicidal effects of fungal chitosan isolated from Penicillium waksmanii and Penicillium citrinum

  • Mahdi Fakhar
  • Aroona Chabra
  • Bahman Rahimi-Esboei
  • Fatemeh Rezaei
Original Article

Abstract

Hydatidosis is caused by a tapeworm which infects humans by the larval stage. In humans, the disease is so serious that it requires surgery for treatment. Documents show that there have been many efforts in finding new scolicidal agents for reducing the rate of the infection. The objective of this study was determination of the scolicidal effect of two fungal chitosan types, produced from Penicillium spp. and commercially chitosan (CC) on Echinococcus granulosus protoscolex. Protoscolices were aseptically aspirated from sheep livers hydatid cysts. Four concentrations (50, 100, 200, 400 μg/ml) of each type of prepared chitosan were used for 10, 30, 60 and 180 min. Viability of protoscolices was detected by 0.1 % eosin staining. Fungal chitosan which was the most bioactive type with higher degree of deacetylation showed stronger scolicidal activity in vitro (P < 0.05). Fungal chitosan could be recommended, as good as CC for hydatid cysts control and is a noble alternative for synthetic and chemical scolicidal.

Keywords

Chitosan Protoscolices Echinococcus granulosus Penicillium 

Notes

Acknowledgments

The authors would like to appreciate Dr. Mohammad Ali Ebrahimzadeh for his precious directions. This work was supported by a Grant from Student Research Committee of Mazandaran University of Medical Sciences, Sari, Iran.

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Copyright information

© Indian Society for Parasitology 2013

Authors and Affiliations

  • Mahdi Fakhar
    • 1
  • Aroona Chabra
    • 2
  • Bahman Rahimi-Esboei
    • 3
  • Fatemeh Rezaei
    • 3
  1. 1.Molecular and Cell Biology Research CenterSchool of Medicine, Mazandaran University of Medical SciencesSariIran
  2. 2.Student Research CommitteeSchool of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical SciencesSariIran
  3. 3.Toxoplasmosis Research CenterSchool of Medicine, Mazandaran University of Medical SciencesSariIran

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