Silicon

pp 1–7 | Cite as

Characterization of Silicon Accumulation in Maize Cell Suspension Cultures

  • Hagit Nissan
  • Shula Blum
  • Eyal Shimoni
  • Rivka Elbaum
Original Paper

Abstract

Purpose

Silicon (Si) is an abundant element in the earth’s crust and is available to plants as silicic acid. Silicon uptake by plants is correlated with increased tolerance to various biotic and abiotic stresses. However, cellular mechanisms responsible for its beneficial effects are still unknown. Even its cellular import mechanisms are not well understood. We thus aimed to characterize silicon localization within minimally differentiated Zea mays (Black Mexican Sweet) cells in suspension.

Methods

Cells were grown in a medium containing silicon, and the mRNA levels of silicon transporters were measured by real-time PCR. Cells were separated into an insoluble (mainly walls and starch) and a cytoplasmic fraction. Soluble and total silicon was measured by inductively-coupled-plasma – atomic-emission-spectroscopy. Silicon distribution was assessed by transmission electron microscopy. The cell walls were analyzed chemically, and by Raman micro-spectroscopy and thermal gravimetric analysis.

Results

Silicon treatment reduced the levels of silicon transporters transcripts, without affecting cell proliferation. About 70 % of the silicon was localized in the cytoplasm, mostly in vesicles. We found indications that silicon affected the secondary structure of proteins and thermally stabilized starch. Silicon was loosely bound, and diffused out of the cells within 24 hours.

Conclusions

Our results show that silicon binds spontaneously to cell walls/starch and accumulates in cytoplasm vesicles. These processes allow the cells to accumulate silicon against its concentration gradient in solution. However, cellular intake acts against reversible diffusion processes, probably through the aquaporin silicon channels (Lsi1, Lsi6) that exchange the cellular silicon with the surrounding medium.

Keywords

Silicon Cell suspension Black Mexican Sweet (BMS) Thermal gravimetric analysis (TGA) Raman microspectroscopy Cell wall 

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Supplementary material

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References

  1. 1.
    Bockhaven JV, Vleesschauwer DD, Höfte M (2013) Towards establishing broad-spectrum disease resistance in plants: silicon leads the way. J Exp Bot 64:1281–1293. doi:10.1093/jxb/ers329 CrossRefGoogle Scholar
  2. 2.
    Ma JF (2010) Silicon transporters in higher plants. Adv Exp Med Biol 679:99–109CrossRefGoogle Scholar
  3. 3.
    Neumann D, Figueiredo CD (2002) A novel mechanism of silicon uptake. Protoplasma 220:59–67. doi:10.1007/s00709-002-0034-7 CrossRefGoogle Scholar
  4. 4.
    Lawton JR (1980) Observations on the structure of epidermal cells, particularly the cork and silica cells, from the flowering stem internode of Lolium temulentum L. (Gramineae). Bot J Linn Soc 80:161–177. doi:10.1111/j.1095-8339.1980.tb01663.x CrossRefGoogle Scholar
  5. 5.
    Gong HJ, Randall DP, Flowers TJ (2006) Silicon deposition in the root reduces sodium uptake in rice (Oryza sativa L.) seedlings by reducing bypass flow. Plant Cell Environ 29:1970–1979. doi:PCE1572 CrossRefGoogle Scholar
  6. 6.
    He C, Ma J, Wang L (2015) A hemicellulose-bound form of silicon with potential to improve the mechanical properties and regeneration of the cell wall of rice. New Phytol 206:1051–1062. doi:10.1111/nph.13282 CrossRefGoogle Scholar
  7. 7.
    Zhang C, Wang L, Zhang W, Zhang F (2013) Do lignification and silicification of the cell wall precede silicon deposition in the silica cell of the rice (Oryza sativa L.) leaf epidermis Plant Soil 372:137–149. doi:10.1007/s11104-013-1723-z CrossRefGoogle Scholar
  8. 8.
    Mccann M C, Wells B, Roberts K (1990) Direct visualization of cross-links in the primary plant cell wall. J Cell Sci 96:323–334Google Scholar
  9. 9.
    Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597. doi:10.3168/jds.S0022-0302(91)78551-2 CrossRefGoogle Scholar
  10. 10.
    Mitani N, Chiba Y, Yamaji N, Ma JF (2009) Identification and characterization of maize and barley Lsi-2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice. Plant Cell 21:2133–2142. doi:10.1105/tpc.109.067884 CrossRefGoogle Scholar
  11. 11.
    Mitani N, Yamaji N, Ma JF (2009) Identification of maize silicon influx transporters. Plant Cell Physiol 50:5–12. doi:10.1093/pcp/pcn110 CrossRefGoogle Scholar
  12. 12.
    He C, Wang L, Liu J et al (2013) Evidence for “silicon” within the cell walls of suspension-cultured rice cells. New Phytol 200:700–709. doi:10.1111/nph.12401 CrossRefGoogle Scholar
  13. 13.
    Sangster AG (1970) Intracellular silica deposition in immature leaves in three species of the Gramineae. Ann Bot 34:245–257Google Scholar
  14. 14.
    Barreto PLM, Pires ATN, Soldi V (2003) Thermal degradation of edible films based on milk proteins and gelatin in inert atmosphere. Polym Degrad Stab 79:147–152. doi:10.1016/S0141-3910(02)00267-7 CrossRefGoogle Scholar
  15. 15.
    Greenwood CT, Muirhead HE (1967) The thermal degradation of starch part VII. Differential thermal analysis of maltodextrins and of starch and its components. Starch - Stärke 19:281–285. doi:10.1002/star.19670190902 CrossRefGoogle Scholar
  16. 16.
    Ramiah MV (1970) Thermogravimetric and differential thermal analysis of cellulose, hemicellulose, and lignin. J Appl Polym Sci 14:1323–1337. doi:10.1002/app.1970.070140518 CrossRefGoogle Scholar
  17. 17.
    Wang W, Wang Y, Yang L, et al. (2005) Studies on thermal behavior of reconstituted tobacco sheet. Thermochim Acta 437:7–11. doi:10.1016/j.tca.2005.06.002 CrossRefGoogle Scholar
  18. 18.
    Choi SS, Kim MC, Kim YK (2011) Influence of silica on formation of levoglucosan from carbohydrates by pyrolysis. J Anal Appl Pyrolysis 90:56–62. doi:10.1016/j.jaap.2010.10.009 CrossRefGoogle Scholar
  19. 19.
    Gierlinger N, Keplinger T, Harrington M (2012) Imaging of plant cell walls by confocal Raman microscopy. Nat Protoc 7:1694–1708. doi:10.1038/nprot.2012.092 CrossRefGoogle Scholar
  20. 20.
    Williams RW (1983) Estimation of protein secondary structure from the laser Raman amide I spectrum. J Mol Biol 166:581–603. doi:10.1016/S0022-2836(83)80285-X CrossRefGoogle Scholar
  21. 21.
    Susi H, Byler DM (1988) Fourier deconvolution of the amide I Raman band of proteins as related to conformation. Appl Spectrosc 42:819–826CrossRefGoogle Scholar
  22. 22.
    Norde W, Giacomelli CE (2000) BSA structural changes during homomolecular exchange between the adsorbed and the dissolved states. J Biotechnol 79:259–268. doi:10.1016/S0168-1656(00)00242-X CrossRefGoogle Scholar
  23. 23.
    Eglin D, Shafran KL, Livage J et al (2006) Comparative study of the influence of several silica precursors on collagen self-assembly and of collagen on “Si” speciation and condensation. J Mater Chem 16:4220–4230. doi:10.1039/B606270A CrossRefGoogle Scholar
  24. 24.
    Mathé C, Devineau S, Aude J-C et al (2013) Structural determinants for protein adsorption/non-adsorption to silica surface. PLoS ONE 8:e81346. doi:10.1371/journal.pone.0081346 CrossRefGoogle Scholar
  25. 25.
    Stavinskaya ON, Laguta IV, Kuzema PA (2011) Effect of highly dispersed silica on water absorption of gelatin materials. Prot Met Phys Chem Surf 47:302–306. doi:10.1134/S2070205111030154 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Hagit Nissan
    • 1
  • Shula Blum
    • 1
  • Eyal Shimoni
    • 2
  • Rivka Elbaum
    • 1
  1. 1.The Robert H. Smith Institute of Plant Sciences and Genetics in AgricultureThe Hebrew University of JerusalemRehovotIsrael
  2. 2.Department of Chemical Research SupportWeizmann Institute of ScienceRehovotIsrael

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