# Diagnosing metabolic acidosis in the critically ill: bridging the anion gap, Stewart, and base excess methods

- 5.5k Downloads
- 29 Citations

## Abstract

### Purpose

Metabolic acid–base disorders are common in critically ill patients. Clinicians may have difficulty recognizing their presence when multiple metabolic acid–base derangements are present in a single patient. Clinicians should be able to identify the components of complex metabolic acid–base disorders since metabolic acidoses due to unmeasured anions are associated with increased mortality in critically ill patients. This review presents the derivation of three commonly used methods of acid–base analysis, which include the anion gap, Stewart physiochemical, and modified base excess. Clinical examples are also provided to demonstrate the subtleties of the different methods and to demonstrate their application to real patient data.

### Principal findings

A comparison of these methods shows that each one is equally adept at identifying a metabolic acidosis due to unmeasured anions; however, the Stewart physiochemical and the modified base excess methods better evaluate complex metabolic acid–base disorders.

### Conclusions

While all three methods correctly identify metabolic acidosis due to unmeasured anions, which is a predictor of mortality, it remains unclear if further delineation of complex metabolic acid–base disorders using the Stewart physiochemical or the modified base excess methods is clinically beneficial.

## Keywords

Metabolic Acidosis Metabolic Alkalosis Strong Anion Base Disorder Unmeasured Anion# Le dépistage de l’acidose métabolique chez les patients gravement malades: un pont entre les méthodes du trou anionique, du modèle de Stewart et de l’excès de base

## Résumé

### Objectif

Les troubles acidobasiques métaboliques sont répandus chez les patients gravement malades. Les cliniciens pourraient rencontrer des difficultés quant à la détection de leur présence lorsque de nombreux troubles acidobasiques métaboliques se manifestent chez un seul patient. Les cliniciens devraient être en mesure de déterminer les composantes des troubles acidobasiques métaboliques complexes, étant donné que les acidoses métaboliques causées par un niveau non mesuré d’anions sont associées à une mortalité accrue chez les patients gravement malades. Cette revue présente la dérivation de trois méthodes couramment utilisées pour réaliser une analyse acidobasique: le trou anionique, la méthode physiochimique de Stewart et l’excès de base modifié. Des exemples cliniques sont également fournis pour démontrer les subtilités des différentes méthodes ainsi que leur application à des données de patients réelles.

### Constatations principales

Une comparaison de ces méthodes montre qu’elles sont toutes efficaces pour dépister une acidose métabolique causée par des anions non mesurés ; toutefois, les méthodes physiochimique de Stewart et d’excès de base modifié permettent une meilleure évaluation des troubles acidobasiques métaboliques complexes.

### Conclusion

Bien que les trois méthodes détectent de façon adéquate l’acidose métabolique causée par des anions non mesurés, un prédicteur de mortalité, il reste à déterminer si une délinéation plus poussée des troubles acidobasiques métaboliques avec les méthodes physiochimique de Stewart et d’excès basique modifié présente des avantages cliniques.

## Introduction

In the early 1900s, sufficient laboratory and observational evidence had been accumulated to define the influence of carbon dioxide on pH1 and to suggest a role for serum bicarbonate in characterizing acid–base disorders.2 Henderson recognized that carbon dioxide and bicarbonate were key elements of carbonate mass action, as shown by his famous equilibrium equation.3 Hasselbalch reformulated this equation by introducing the negative logarithmic pH notation and by applying Henry’s Law to generate the pCO_{2} term.4 It was quickly appreciated, however, that the Henderson–Hasselbalch equation failed to account for the influence of non-bicarbonate buffers and serum electrolytes on acid–base interpretation.5 Additionally, this equation lists pCO_{2} and HCO_{3} ^{−} as independent predictors of pH when, in fact, these variables are interdependent. Consequently, this equation merely serves as a description of a patient’s acid–base status but does not provide insight into the mechanism of the patient’s acid–base disorder.

In the 1950s, Siggaard-Anderson sought to stay faithful to the bicarbonate approach to acid–base analysis while distinguishing the metabolic and the respiratory contributions.6 At a fixed temperature and partial pressure of carbon dioxide, these investigators measured the plasma bicarbonate concentration and compared the difference between this value and a reference. When corrected by a constant, this difference yields the base excess (BE). Clinically, this BE represents the amount of acid per unit volume that must be added to achieve a normal pH. Criticism of this method soon followed.7,8 For example, the laboratory BE value represents the net effect of all metabolic acid–base abnormalities. Therefore, the effect of co-existing metabolic acidoses and alkaloses may cancel to falsely suggest that no acid–base abnormality exists. In fact, two large series have observed a normal BE in one-sixth of critically ill patients with acid–base disorders.9,10 Furthermore, this BE does not suggest an etiology for the acid–base disorder once an abnormality is discovered.

Two alternative theoretical approaches, the anion gap (AG) method and the Stewart physiochemical method of acid–base analysis, were eventually introduced.11,12 Each of these methods provides the means to identify co-existing acid–base disorders. The modified base excess method was developed later as a simplified version of the Stewart physiochemical approach. Despite the perceived differences between these three methods, they all share a common theoretical foundation based on the principles of electroneutrality and the role of plasma weak acids.

This review presents the theory and the clinical application of each of these three methods of acid–base analysis: the AG, the Stewart physiochemical, and the modified base excess. This review further compares the ability of each method to correctly diagnose metabolic acid–base disorders in critically ill patients. Current data are presented to determine if any one method is more advantageous in clinical practice. Clinical examples are given to highlight the ability of each approach to reliably recognize complex metabolic acid–base disorders in critically ill patients.

## The anion gap method of acid–base analysis

*K*

_{a}that is many orders of magnitude less than plasma pH, completely dissociate into the conjugate base (XA

^{−}) and a proton (H

^{+}). The proton subsequently joins with buffer to create a neutral species, and the conjugate base remains to signal the presence of the acid species. In other words, the conjugate base can be thought of as the footprint of the strong acid. The dissociation of strong acids changes the relative composition of plasma anionic species with a decrease in buffer and with an increase in conjugate base; however, electroneutrality still remains.15 This process is illustrated in Fig. 1. Increased amounts of conjugate base are reflected in an increased AG when the strong acid is organic.

^{−}, SO

_{4}

^{2−}, CO

_{3}

^{2−}, and H

^{+}are significantly small that their contributions to the electroneutrality equation can be ignored. The plasma concentrations of Na

^{+}, K

^{+}, Cl

^{−}, and HCO

_{3}

^{−}are reported in a standard chemistry panel. The sum of the remaining anion species, protein

^{−}, PO

_{4}

^{2−}, and XA

^{−}, is defined as the unmeasured anions (UA), whereas the sum of the remaining cation species, Mg

^{2+}and Ca

^{2+}, is defined as the unmeasured cations (UC). Simple algebraic rearrangement of the electroneutrality equation yields the expression of the AG as

A normal AG is 12 ± 4 mEq/l.13 The presence of organic acid (XAH), which dissociates to form the anionic species XA^{−} while consuming bicarbonate, results in increased UA and a subsequently increased AG. Therefore, an incremental increase in the calculated AG compared to the institutional reference suggests the presence of an organic metabolic acidosis.11,13,14

*K*

_{a}is much greater than plasma pH. Furthermore, the charge contribution of albumin is significantly greater than that of phosphates. In fact, plasma pH is shown to be linearly related to plasma albumin concentration.26 Based on these results, the AG can be corrected based on the albumin concentration. This corrected AG (AG

_{Corr}), as determined empirically,11,13,16, 17, 18 is given by

_{Corr}can unmask an organic acidosis that was previously undetected in the setting of hypoalbuminemia.

While the AG is an important indicator of metabolic acidosis, not all metabolic acidoses result in an elevated AG. Consider an example in which acid in the form of HCl is added to the plasma. Upon dissociation, Cl^{−} remains as the conjugate base while the bicarbonate buffer is consumed by the proton. As a result, an increase in [Cl^{−}] is balanced by a decrease in [HCO_{3} ^{−}].

Critically ill patients rarely have a single metabolic acid–base disorder. These patients typically manifest mixed acid–base physiology. The concept of the delta–delta can be used to elucidate mixed acid–base disorders.32 The delta–delta compares the delta corrected AG (ΔAG_{Corr}), which is defined as the difference between the calculated AG_{Corr} and the reference AG, to the delta [HCO_{3} ^{−}] (ΔHCO_{3} ^{−}), which is defined as the difference between the reference [HCO_{3} ^{−}] and the measured [HCO_{3} ^{−}]. As classically described, an incremental increase in AG_{Corr} should be mirrored by the same incremental decrease in [HCO_{3} ^{−}]; such that ΔAG_{Corr} should equal ΔHCO_{3} ^{−} if only an AG metabolic acidosis is present. However, this simplistic 1:1 ratio of ΔAG_{Corr} to ΔHCO_{3} ^{−} fails to consider the role of non-bicarbonate buffers, assumes the same volume of distribution for both the conjugate base and the proton, and disregards the duration of acidosis.33,34 Taking these three conditions into account, the actual ratio of ΔAG_{Corr} to ΔHCO_{3} ^{−} is quite variable depending on the acid species that is present.35,36 A review of the literature shows that this ratio ranges from 0.8:1 to 1.8:1 for lactate and from <0.8:1 to 1:1 for ketoacids and toluene.37 For simplicity, the range of (1 to 1.6):1 can be used in the delta–delta calculations. To appreciate the clinical usefulness of the delta–delta, consider the case in which the ΔAG_{Corr} is 10 mEq/l. Based on the (1 to 1.6:1) ratio of ΔAG_{Corr} to ΔHCO_{3} ^{−}, the expected [HCO_{3} ^{−}] should be approximately 6–10 mEq/l less than the reference [HCO_{3} ^{−}]. A measured [HCO_{3} ^{−}] that is above this range of expected values suggests a co-existing metabolic alkalosis, whereas a measured [HCO_{3} ^{−}] that is below this range of expected values suggests a co-existing non-AG metabolic acidosis.

## The Stewart method of acid–base analysis

Peter Stewart developed a quantitative physiochemical model to explain acid–base physiology.5,12,38,39 This method is purely a mathematical analysis of a physiologic system, which lays the foundation for understanding acid–base disorders. He defined his system as follows: a solution that contains strong ions that are completely dissociated at physiologic pH, weak acids that are partially dissociated at physiologic pH, and carbon dioxide that is in equilibrium with an external partial pressure of carbon dioxide. He represented his model by a system of equations that satisfied the dissociation equilibrium of the partially dissociated species, conservation of mass, and electroneutrality. While a complete understanding of this model is not necessary for clinical acid–base analysis, it is important to understand a few basic principles that are derived from Stewart’s analysis. First, Stewart identified only three independent variables, specifically, the strong ion difference (SID), the total concentration of weak acids (A_{TOT}), and the partial pressure of carbon dioxide (pCO_{2}). Second, only changes in these independent variables will result in changes in the dependent variables [H^{+}] and [HCO_{3} ^{−}]. Therefore, derangements in acid–base balance can only occur with changes in SID, A_{TOT}, and pCO_{2}.

Since pCO_{2} is regulated by respiration, changes in pCO_{2} result in respiratory acid-base disorders. Metabolic acid–base disorders result from changes in either A_{TOT} or SID. The clinical meaning of the variables SID and A_{TOT} and the concept of how changes in these variables affect metabolic acid–base balance are reviewed here.

^{+}, K

^{+}, Ca

^{2+}, and Mg

^{2+}, and the strong anion is Cl

^{−}. Therefore, the apparent SID is defined as

^{−}]) will change the SID such that the effective SID (SID

_{Eff}) becomes

^{−}] is of clinical significance, since it is indicative of an organic acidosis. These unmeasured anions can be quantified by the strong ion gap (SIG),5,40 which is defined as

_{App}is calculated by Eq. 4. An approximation for SID

_{Eff}is calculated from the electroneutrality equation (Eq. 1) by rearranging the terms and ignoring the negligible terms ([OH

^{−}], [SO

_{4}

^{2−}], [CO

_{3}

^{2−}], and [H

^{+}]), such that,

^{−}] and [PO

_{4}

^{2−}] can be calculated based on the plasma concentrations for albumin and phosphate, as reported by the laboratory,9 such that

_{App}and SID

_{Eff}are determined with routine laboratory studies. As a result, [XA

^{−}] is easily approximated based on the SIG.

Based on the previous discussion, the strong anions are conjugate bases, which are the footprint acids. Similarly, the strong cations are the conjugate acids, which are the footprints of bases. Therefore, an increase in SID, which indicates a net increase in cations, results in a metabolic alkalosis. A decrease in SID, which indicates a net increase in anions, results in a metabolic acidosis. At physiologic pH, which is slightly alkaline at about 7.44, the SID is positive.

From the above discussion, it becomes apparent that changes in the independent variable SID will result in alterations in acid–base balance. By understanding how SID can change, one can better appreciate the cause of a given acid–base disorder. Changes in SID result from either a relative or an absolute change in strong ion concentrations.

An alteration in the relative concentration of the strong ions, and a subsequent change in SID, occurs with a change in free water content. For example, if enough free water is added to dilute the strong ion concentrations in half, the SID will also decrease by half. To better understand this concept, consider a patient with Na^{+}, K^{+}, Ca^{2+}, Mg^{2+}, and Cl^{−} concentrations (mEq/l) of 140, 4, 6, 2, and 102, respectively. The SID for this patient is 50 mEq/l (140 + 4 + 6 + 2 − 102). By adding enough free water to decrease these concentrations by half, the SID becomes 25 mEq/l (70 + 2 + 3 + 1 − 51). Thus, excess free water lowers SID and results in a metabolic acidosis. By a similar analysis, a free water deficit causes a metabolic alkalosis by increasing the SID through a relative increase in concentration of all strong ions.

According to Stewart, A_{TOT} is the second independent variable that affects metabolic acid–base balance. A_{TOT} is defined as the total amount of weak acid species in both the dissociated and the non-dissociated form. In physiologic systems, the major weak acids are albumin and phosphate. An increase in the weak acid concentration results in a metabolic acidosis. The primary examples are hyperphosphatemia in renal failure and hyperalbuminemia of hemoconcentration. A decrease in weak acid concentration results in a metabolic alkalosis. The primary example is hypoalbuminemia due to malnutrition, liver cirrhosis, or nephrotic syndrome. Since normal serum phosphate concentration is relatively small, hypophosphatemia alone will not result in a significant metabolic alkalosis.

Based on the Stewart method, metabolic acid–base disorders can be elucidated by analyzing changes in SID and A_{TOT}. The differential diagnosis for metabolic acid–base disturbances can be determined based on changes in SID and A_{TOT}, as shown in Fig. 3.

## The base excess method of acid–base analysis

The classical base excess theory is limited by its inability to differentiate co-existing metabolic acid–base disorders. Gilfix et al*.* describe a base-excess method, which is based on the fundamental principles of Stewart’s physiochemical model, to quantify the metabolic component of acid–base disorders.41 Based on an analysis of Stewart’s model, Gilfix proposed that only four conditions create non-respiratory acid–base disturbances. These conditions are: (1) a free water deficit or excess, as determined by changes in sodium concentration; (2) changes in chloride concentration; (3) changes in protein charges, mainly albumin; and (4) the presence of organic unmeasured anions.

_{2}of 40 mmHg and a temperature of 37°C to achieve a neutral pH. A positive BE indicates an alkalosis, whereas a negative BE indicates an acidosis. The BE is readily reported by a clinical laboratory. Gilfix et al

*.*show that the BE reported by the laboratory should equal the sum of the BE for each of the four conditions listed above, such that,

_{lab}are calculated as follows:41,42

^{−}] represents the unmeasured anions, BE

_{XA}is calculated from Eq. 9 once the other four quantities are known.

This base excess method of acid–base analysis provides a simple method that can be readily used at the bedside to determine the non-respiratory disturbances in a patient’s acid–base status.

## Comparison of the three methods of acid–base analysis

Critically ill patients manifest complex acid–base disorders. While the respiratory component is readily diagnosed by analyzing pCO_{2}, the metabolic component requires a more complex analysis. The three methods that are described above are used clinically to diagnose and understand a patient’s acid–base status. The question arises as to which method is the most useful in the clinical setting. In order to understand the answer to this question, one must look at predictors of mortality in critically ill patients and consider which method of acid–base analysis correlates well with mortality in these patients.

In a retrospective review of 851 critically ill patients with a documented metabolic acidosis, Gunnerson et al. show that mortality associated with lactic acidosis (56%) and non-lactate strong anion acidosis (39%) is significantly higher than that associated with hyperchloremic acidosis (29%) and controls (25%).43 Therefore, the identification of unmeasured anions is crucial in any acid–base analysis in critically ill patients. Using the three methods described above, the unmeasured anions ([XA^{−}]) are identified by the AG_{Corr}, SIG, and BE_{XA}, respectively. Several studies have looked at the correlation of these different measures of [XA^{−}] with mortality. Balasubmarmanyan et al*.* show that BE_{XA} correlates with mortality in pediatric ICU patients,42 whereas Cusack et al*.* do not find that correlation in adult ICU patients.44 Since the results of these two studies are limited by a small sample size, Rocktaeschel et al*.* retrospectively reviewed 300 adult ICU patients.45 This study shows that the unmeasured anions were the only acid–base variables that had limited prediction of mortality in critically ill patients. They further show a strong correlation between all methods of calculating the unmeasured anions (AG, AG_{Corr}, SIG, and BE_{XA}).

This correlation between indicators of unmeasured anions is confirmed by several clinical studies. Kellum et al*.* demonstrate a tight correlation between SIG and AG_{Corr}, but not AG, in 75 ICU patients with sepsis (*r* = 0.93) and those with severe liver disease (*r* = 0.91).46 Moviat et al*.* also show a strong correlation between SIG and AG_{Corr} (*R* ^{2} = 0.93) in 50 consecutive ICU patients with a documented metabolic acidosis.47 More recently, Dubin et al*.* confirm the strong correlation between SIG and AG_{Corr} (*R* ^{2} = 0.97) in a prospective study of 935 patients admitted to a mixed medical–surgical ICU.10 Based on these data, all three methods of acid–base analysis are equally adept at identifying an acidosis due to unmeasured anions, provided that the AG is corrected for hypoalbuminemia.

The AG method is traditionally used and understood by most clinicians. This method, however, requires an empirically derived correction factor to account for the effects of alterations in albumin concentration. Additionally, the simplifying assumptions in the delta–delta calculations of the AG method may hide subtle abnormalities. For example, the AG method does not identify acid–base abnormalities that are due to alterations in plasma free water. Additionally, the AG method does not account for the correction of chloride concentration in the setting of altered plasma free water. As a result, a hyperchloremic acidosis in the setting of a dilutional alkalosis would not be identified with an analysis using the AG method. In contrast, both the Stewart physiochemical method and the modified base excess method are better able to delineate co-existing acid–base disorders. The Stewart physiochemical method, however, is mathematically rigorous and may be difficult to routinely use at the bedside. The modified base excess method is based on the principles of the Stewart physiochemical method but is mathematically simple and can be readily applied at the bedside. It remains unclear, however, if the identification of subtle acid–base abnormalities**,** by either the Stewart physiochemical or the modified base excess method**,** is of clinical significance.

Qualitative changes in physiologic variables with a metabolic acidosis, as determined by the anion gap, Stewart physiochemical, and modified base excess methods of acid–base analysis

Anion gap | Physiochemical | Base excess | |
---|---|---|---|

Free water excess | — | ↓ SID, ↓ [Na | −BE |

Hyperchloremia | ↔ AG, ↑ [Cl | ↓ SID, ↑ [Cl | −BE |

Presence of unidentified anions | ↑ AG | ↓ SID, ↑ SIG | −BE |

Hyperalbuminemia | ↓ AG | ↑ [Albumin] | −BE |

Key equations for diagnosing metabolic acid–base disorders using the anion gap, Stewart physiochemical, and modified base excess methods of acid–base analysis

Anion gap | \( {\text{AG}} \equiv {\text{UA}} - {\text{UC}} = ( [ {\text{Na}}^{ + } ] + [ {\text{K}}^{ + } ]) - ( [ {\text{Cl}}^{ - } ] + [ {{\text{HCO}}_{ 3}}^{ - } ]) \) |

Stewart physiochemical | \( {\text{SID}}_{\text{Eff}} \equiv ( [ {\text{Na}}^{ + } ] + [ {\text{K}}^{ + } ] + [ {\text{Ca}}^{ 2+ } ] + [ {\text{Mg}}^{ 2+ } ]) - ( [ {\text{Cl}}^{ - } ] + [ {\text{XA}}^{ - } ]) = [ {{\text{HCO}}_{ 3}}^{ - } ] + [ {\text{protein}}^{ - } ] + [ {{\text{PO}}_{ 4}}^{ 2- } ] \) |

Modified base excess | \( {\text{BE}}_{\text{lab}} = {\text{BE}}_{\text{fw}} + {\text{BE}}_{\text{Cl}} + {\text{BE}}_{\text{alb}} + {\text{BE}}_{\text{XA}} \) |

## Clinical examples

*.*provide clinical data on the ability of the AG and the physiochemical methods to detect acid–base abnormalities in 152 critically ill patients.9 The laboratory data and the associated calculated values for two representative patients and nine controls are shown in Table 3. The following discussion demonstrates the application of the AG, Stewart, and modified base excess methods to the analysis of the acid–base disturbances in these two sample patients.

Measured and calculated values for two representative patients with acid–base disorders and for nine controls

Patient 88 | Patient 59 | Controls ( | |
---|---|---|---|

| |||

pH | 7.40 | 7.33 | 7.42 |

p | 39 | 30 | 38 |

[Na | 137 | 117 | 142 |

[K | 4.9 | 3.9 | 4.1 |

[Cl | 102 | 92 | 106 |

[Mg | 1.6 | 1.4 | 0.8 |

[Ca | 3.2 | 3.0 | 2.3 |

Albumin (g/l) | 6 | 6 | 44 |

P | 0.3 | 0.6 | 1.0 |

| |||

[HCO | 24 | 15 | 24.5 |

[Cl | 106 | 112 | 106 |

ΔAG | 13 | 11 | 3 |

SIG (mEq/l) | 19 | 18 | 8 |

BE | 0 | −10 | 0.3 |

A cursory analysis of the data for patient 88, who has post-operative multiple organ failure, shows a normal pH, pCO_{2}, and BE, which suggest that no acid–base abnormalities exist. Using the AG method, an acidosis due to unmeasured anions is identified by an elevated AG_{Corr} (ΔAG_{Corr} = 13 mEq/l). Based on the delta–delta calculation, the expected decrement of [HCO_{3} ^{−}] would be 8–13 mEq/l, which corresponds to a [HCO_{3} ^{−}] value of 11–16 mEq/l. However, the actual [HCO_{3} ^{−}] is 24 mEq/l, which suggests a co-existing metabolic alkalosis. An analysis using the Stewart method reveals an elevated SIG, a normal [Na^{+}], a normal [Cl^{−}]_{Corr}, and decreased albumin and phosphate levels. These changes indicate an acidosis due to unmeasured anions with a concomitant hypoalbuminemic and hypophosphatemic alkalosis. The modified base excess approach yields a BE_{lab}, BE_{fw}, and BE_{Cl} that all equal 0 mEq/l. The BE_{alb} is calculated to be 13 mEq/l. Subsequently, the BE_{XA} must equal −13 mEq/l. These results indicate an acidosis due to unmeasured anions and a hypoalbuminemic alkalosis. For this sample patient, each of the three methods correctly identifies each of the metabolic acid–base derangements.

Patient 59 also has post-operative multiple organ failure; however, this patient has a more complex acid–base physiology. The pH of 7.33, pCO_{2} of 30, and negative BE suggest an acidemia, a metabolic acidosis, and a respiratory alkalosis; however, there is no indication as to the etiology of the metabolic acidosis. Using the AG method, an elevated AG_{Corr} (ΔAG_{Corr} = 11 mEq/l) indicates a metabolic acidosis due to unmeasured anions. With the delta–delta calculation, the expected decrement of [HCO_{3} ^{−}] would be 7–11 mEq/l, which corresponds to a [HCO_{3} ^{−}] value of 13–17 mEq/l. The actual [HCO_{3} ^{−}] is 15 mEq/l, which is consistent with the expected value. Therefore, no further metabolic acid–base disorders are appreciated, although, intuitively, a hypoalbuminemic alkalosis is present. The Stewart approach demonstrates an increased SIG, a decreased [Na^{+}], an increased [Cl^{−}]_{Corr}, and decreased albumin and phosphate levels. These results indicate a triple acidosis, which is due to unmeasured anions, free water excess, hyperchloremia, and a concomitant hypoalbuminemic and hypophosphatemic alkalosis. Similarly, the modified base excess method yields a positive BE_{alb} (13 mEq/l), a negative BE_{Cl} (−8 mEq/l), a negative BE_{fw} (−7 mEq/l), and a negative BE_{XA} (−8 mEq/l), which indicate co-existing hypoalbuminemic alkalosis, hyperchloremic acidosis, dilutional acidosis, and unmeasured anion acidosis. For this sample patient, only the Stewart method and the modified base excess method correctly identify all of the metabolic acid–base disorders, whereas the AG method fails to fully identify the hypoalbuminemic alkalosis, the hyperchloremic acidosis, and the dilutional acidosis. The clinical implication of this oversight, however, remains unknown.

## Conclusions

The traditional BE and bicarbonate approaches to acid–base physiology can fail to detect complex metabolic disorders in critically ill patients. While their derivations differ, the AG, Stewart, and modified base excess methods share similar starting assumptions and are equally adept at identifying a metabolic acidosis due to unmeasured anions, which is a predictor of mortality. As demonstrated by the second sample patient, if multiple complex acid–base disorders are present, the Stewart method or the modified base excess method may more effectively delineate the different disorders. It remains unclear if the identification of these additional acid–base disorders justifies the additional effort required for the routine use of the Stewart method. The modified base excess approach may best combine the ease of use with clinical effectiveness while staying true to the principles of acid–base physiology.

## Notes

### Conflicts of interest

None declared.

## References

- 1.
*Hasselbalch KA*. Neutralitatsregulation und reizbarkeit des atemzentrums in ihren wirkungen auf die kohlensaurespannung des blutes. Biochem Z 1912; 46: 403–39.Google Scholar - 2.
*Van Slyke DD*. Some points of acid–base history in physiology and medicine. Ann NY Acad Sci 1966; 133: 5–14.PubMedCrossRefGoogle Scholar - 3.
*Henderson LJ*. Das gleichgewicht zwischen sauren und basen im tierischen organismus. Ergebn Physio 1909; 8: 254–325.Google Scholar - 4.
*Hasselbalch KA*. Die berechnung der wasserstoffzahl des blutes aus der freien und gebundenen kohlensaure desselben und die sauerstoffbindung des blutes als funktion der wasserstoffzahl. Biochem Z 1916; 78: 112–44.Google Scholar - 5.
*Fencl V, Leith DE*. Stewart’s quantitative acid–base chemistry: applications in biology and medicine. Respir Physiol 1993; 91: 1–16.PubMedCrossRefGoogle Scholar - 6.
*Siggaard Andersen O*. Blood acid–base alignment nomogram. Scandinav J Clin Lab Investigat 1963; 15: 211–7.CrossRefGoogle Scholar - 7.
*Schwartz WB, Relman AS*. A critique of the parameters used in the evaluation of acid–base disorders. “Whole-blood buffer base” and “standard bicarbonate” compared with blood ph and plasma bicarbonate concentration. N Engl J Med 1963; 268: 1382–8.PubMedGoogle Scholar - 8.
*Bunker JP*. The great trans-atlantic acid–base debate. Anesthesiology 1965; 26: 591–4.PubMedGoogle Scholar - 9.
*Fencl V, Jabor A, Kaxda A, Figge J*. Diagnosis of metabolic acid–base disturbances in critically ill patients. Am J Respir Crit Care Med 2000; 162: 2246–51.PubMedGoogle Scholar - 10.
*Dubin A, Menises MM, Masevicius FD, et al*. Comparison of three different methods of evaluation of metabolic acid–base disorders. Crit Care Med 2007; 35: 1264–70.PubMedCrossRefGoogle Scholar - 11.
*Emmett M, Narins RG*. Clinical use of the anion gap. Medicine (Baltimore) 1977; 56: 38–54.CrossRefGoogle Scholar - 12.
*Stewart PA*. Modern quantitative acid–base chemistry. Can J Physiol Pharmacol 1983; 61: 1444–61.PubMedGoogle Scholar - 13.
*Gabow PA*. Disorders associated with an altered anion gap. Kidney Intl 1985; 27: 472–83.CrossRefGoogle Scholar - 14.
*Oh MS, Carroll HJ*. The anion gap. N Engl J Med 1977; 297: 814–7.PubMedGoogle Scholar - 15.
*Singer RB, Hastings AB*. An improved clinical method for the estimation of disturbances of the acid–base balance of human blood. Medicine 1948; 27: 223–42.PubMedCrossRefGoogle Scholar - 16.
*Feldman M, Soni N, Dickson B*. Influence of hypoalbuminemia or hyperalbuminemia on the serum anion gap.*J Lab Clin Med*2005; 146: 317–20.PubMedCrossRefGoogle Scholar - 17.
*Figge J, Jabor A, Kazda A, Fencl V*. Anion gap and hypoalbuminemia. Crit Care Med 1998; 26: 1807–10.PubMedGoogle Scholar - 18.
*Carvounis CP, Feinfeld DA*. A simple estimate of the effect of the serum albumin level on the anion gap. Am J Nephrol 2000; 20: 369–72.PubMedCrossRefGoogle Scholar - 19.
*Gabow PA, Kaehny WD, Fennessey PV, Goodman SI, Gross PA, Schrier RW*. Diagnostic importance of an increased serum anion gap. N Engl J Med 1980; 303: 854–8.PubMedGoogle Scholar - 20.
*Nanji AA, Campbell DJ, Pudek MR*. Decreased anion gap associated with hypoalbuminemia and polyclonal gammopathy. JAMA 1981; 246: 859–60.PubMedCrossRefGoogle Scholar - 21.
*McAuliffe JJ, Lind LJ, Leith DE, Fencl V*. Hypoproteinemic alkalosis. Am J Med 1986; 81: 86–90.PubMedCrossRefGoogle Scholar - 22.
*Van Slyke DD, Hastings AB, Hiller A, Sendroy J Jr*. Studies of gas and electrolyte equilibria in blood. XIV. The amounts of alkali bound by serum albumin and globulin. J Biol Chem 1928; 79: 769–80.Google Scholar - 23.
*Van Leeuwen AM*. Net cation equivalency (“base binding power”) of the plasma proteins: a study of ion-protein interaction in human plasma by means of in vivo ultrafiltration and equilibrium dialysis. Acta Med Scand 1964; 422(Suppl): 1–212.Google Scholar - 24.
*Figge J, Rossing TH, Fencl V*. The role of serum proteins in acid–base equilibria. J Lab Clin Med 1991; 117: 453–67.PubMedGoogle Scholar - 25.
*Figge J, Mydosh T, Fencl V*. Serum proteins and acid–base equilibria: a follow-up. J Lab Clin Med 1992; 120: 713–9.PubMedGoogle Scholar - 26.
*Rossing TH, Maffeo N, Fencl V*. Acid–base effects of altering plasma protein concentration in human blood in vitro. J Appl Physiol 1986; 61: 2260–5.PubMedGoogle Scholar - 27.
*Rose BD*. Clinical physiology of acid–base and electrolyte disorders. 4th ed. New York: McGraw-Hill; 2001.Google Scholar - 28.
*Morris CG, Low J*. Metabolic acidosis in the critically ill: part 2. Causes and treatment. Anaesthesia. 2008;63:396–411.PubMedCrossRefGoogle Scholar - 29.
*Williams EL, Hildebrand KL, McCormich SA, Bedel MJ*. The effect of intravenous lactated Ringer’s solution versus 0.9% sodium chloride solution on serum osmolality in human volunteers. Anesth Analg. 1999; 88: 999–1003.PubMedCrossRefGoogle Scholar - 30.
*Scheingraber S, Rehm M, Sehmisch C, Finsterer U*. Rapid saline infusion produces hyperchloremic acidosis in patients undergoing gynecologic surgery. Anesthesiology 1999; 90: 1265–70.PubMedCrossRefGoogle Scholar - 31.
*Heird WC, Dell RB, Driscoll JM Jr, Grebin B, Winters RW*. Metabolic acidosis resulting from intravenous alimentation mixtures containing synthetic amino acids. N Engl J Med 1972; 287: 943–8.PubMedCrossRefGoogle Scholar - 32.
*Narins RG, Emmett M*. Simple and mixed acid–base disorders: a practical approach. Medicine (Baltimore) 1980; 59: 161–87.Google Scholar - 33.
*Salem MM, Mujais SK*. Gaps in the anion gap. Arch Intern Med 1992; 152: 1625–9.PubMedCrossRefGoogle Scholar - 34.
*Schwartz WB, Orning KJ, Porter R*. The internal distribution of hydrogen ions with varying degrees of metabolic acidosis. J Clin Invest 1957; 36(3): 373–82.PubMedCrossRefGoogle Scholar - 35.
*Oh MS, Carroll HJ, Goldstein DA, Fein IA*. Hyperchloremic acidosis during the recovery phase of diabetic ketosis. Ann Intern Med 1978; 89: 925–7.PubMedGoogle Scholar - 36.
*Reilly RF, Anderson RJ*. Interpreting the anion gap. Crit Care Med 1998; 26: 1171–2.Google Scholar - 37.
*Kraut JA, Madias NE*. Serum anion gap: its uses and limitations in clinical medicine. Clin J Am Soc Nephrol. 2007; 2: 162–74.PubMedCrossRefGoogle Scholar - 38.
*Stewart PA*. How to understand acid–base—a quantitative primer for biology and medicine. North Holland: Elsevier; 1981.Google Scholar - 39.
*Jones NL*. A quantitative physiochemical approach to acid–base physiology. Clin Biochem 1990; 23: 189–95.PubMedCrossRefGoogle Scholar - 40.
*Sirker AA, Rhodes A, Grounds RM, Bennett ED*. Acid–base physiology: the ‘traditional’ and the ‘modern’ approaches. Anaesthesia 2002; 57: 348–56.PubMedCrossRefGoogle Scholar - 41.
*Gilfix BM, Bique M, Magder S*. A physical chemical approach to the analysis of acid–base balance in the clinical setting. J Crit Care 1993; 8: 187–97.PubMedCrossRefGoogle Scholar - 42.
*Balasubramanyan N, Havens PL, Hoffman GM*. Unmeasured anions identified by the Fencl–Stewart method predict mortality better than base excess, anion gap, and lactate in patients in the pediatric intensive care unit. Crit Care Med 1999; 27: 1577–81.PubMedCrossRefGoogle Scholar - 43.
*Gunnerson KJ, Saul M, He S, Kellum JA*. Lactate versus non-lactate metabolic acidosis: a retrospective outcome evaluation of critically ill patients. Crit Care 2006; 10: R22–30.PubMedCrossRefGoogle Scholar - 44.
*Cusack RJ, Rhodes A, Lochhead P, et al*. The strong ion gap does not have prognostic value in critically ill patients in a mixed medical/surgical adult ICU. Intensive Care Med 2002; 28: 864–9.PubMedCrossRefGoogle Scholar - 45.
*Rocktaeschel J, Morimatsu H, Uchino S, Bellomo R*. Unmeasured anions in critically ill patients: can they predict mortality? Crit Care Med 2003; 31: 2131–6.PubMedCrossRefGoogle Scholar - 46.
*Kellum JA, Kramer DJ, Pinsky MR*. Strong ion gap: a methodology for exploring unexplained anions. J Crit Care 1995; 10: 51–5.PubMedCrossRefGoogle Scholar - 47.
*Moviat M, van Haren F, van der Hoeven H*. Conventional or physiochemical approach in intensive care unit patients with metabolic acidosis. Crit Care 2003; 7: R41–5.PubMedCrossRefGoogle Scholar