The journal of nutrition, health & aging

, Volume 22, Issue 10, pp 1281–1285 | Cite as

Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals

  • J. Passador
  • L. V. Toffoli
  • K. B. Fernandes
  • R. D. Neves-Souza
  • Gislaine Garcia PelosiEmail author
  • M. V. Gomes



Several lines of evidence from the last decade support the connection between nutrition and epigenetic mechanisms. In the present study we evaluated the impact of the daily dietary intake of calories and the micronutrients vitamin A, D, B1, B2, B5, C, E, copper, calcium, phosphorus, iron, iodine, selenium, manganese, potassium and sodium on the global DNA methylation profile of blood cells from older individuals.

Research Methods & Procedures

The study enrolled 126 physically independent elderly of both sexes (60 men and 66 women). For the molecular analysis, DNA samples were extracted from leukocytes and global DNA methylation was evaluated using a high throughput Elisa-based method. Correlations between global DNA methylation and the daily intake of calorie or micronutrients were evaluated using Prism5 GraphPad Software.


A statistically significant correlation was observed between global DNA methylation and the daily caloric value (p=0.019, r=-0.21), and the intake of vitamin A (p=0.03, r=-0.18), Vitamin E (p=0.027, r=-0.20) and copper (p=0.04, r=-0.18). No correlation was observed between global DNA methylation and the daily intake of vitamin D, B1, B2, B5, C, calcium, phosphorus, iron, iodine, selenium, manganese and potassium (p>0.05).


Our data demonstrate that the daily intake of calories or the micronutrients vitamin A, vitamin E and copper can potentially modulate the global DNA methylation profile of leukocytes in older adults and corroborate the notion of nutritional influences on epigenetic mechanisms.

Key words

DNA methylation dietary modulation older individuals epigenetics 


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Copyright information

© Serdi and Springer-Verlag France SAS, part of Springer Nature 2018

Authors and Affiliations

  • J. Passador
    • 1
  • L. V. Toffoli
    • 1
  • K. B. Fernandes
    • 1
  • R. D. Neves-Souza
    • 1
  • Gislaine Garcia Pelosi
    • 2
    • 3
    Email author
  • M. V. Gomes
    • 1
  1. 1.Research Centre on Health SciencesUniversity of Northern Parana (UNOPAR)LondrinaBrazil
  2. 2.Centre of Biological Sciences, Department of Physiological SciencesState University of Londrina (UEL)LondrinaBrazil
  3. 3.Departamento de Ciências FisiológicasCCB-UEL, Campus UniversitárioLondrinaBrazil

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