The journal of nutrition, health & aging

, Volume 20, Issue 6, pp 586–593 | Cite as

Influence of high polyphenol beverage on stress-induced platelet activation

  • Thomas Nickel
  • K. Lackermair
  • J. Scherr
  • A. Calatzis
  • M. Vogeser
  • H. Hanssen
  • G. Waidhauser
  • U. Schönermark
  • H. Methe
  • S. Horster
  • U. Wilbert-Lampen
  • M. Halle
Article
First Online:
Received:
Accepted:

Abstract

Objectives

Platelets are playing a crucial role in acute cardiovascular events. We investigated if physical stress activates platelets and whether this activation can be inhibited by a polyphenol-enriched diet.

Methods

Blood samples were taken from a total of 103 athletes three weeks before, one day before, immediately as well as 24 hours and 72 hours after a marathon run. Participants were randomized, double-blinded and divided into two groups. One group received a polyphenol-rich beverage the other the same beverage without polyphenols. Besides analysis of platelet counts and impedance-aggregometric-measurement of platelet activity, soluble P-selectin and Endothelin-A measurements were performed.

Results

In the control group, runners showed a 2.2-fold increased platelet aggregation directly after completing a marathon and within the following three days when compared with baseline values (p<0.01). In accordance, significant increases in sP-selectin (57.52ng/ml vs. 94.86ng/ml;p<0.01) were detectable. In contrast, for the group consuming a beverage with increased polyphenol content (upper quartile of study beverage intake) we did not find any increase of platelet aggregation.

Discussion

Physical stress causes a significant increase in platelet activity. Our results demonstrate that a diet enriched in polyphenols is capable of preventing platelet activation. These findings might indicate a diminished cardiovascular stress-reaction following pre-exposition to polyphenol-enriched diet.

Key words

Platelets stress marathon polyphenols 

List of abbreviations

ADP

adenosindiphosphat

AUC

area under the curve

BeMaGIC

Beer Marathon Genetics Inflammation and Cardiovascular System

BNP

brain natriuretic peptide

cAMP

cyclisches Adenosinmonophosphat

CGA

Chromogranin A

ELISA

enzyme linked immunosorbent assay

GbIIb/IIIa

glycol-protein IIb-IIIa complex

GAE

gallic acid equivalents

IQR

interquartile range

NADPH

nicotinamide adenine dinucleotide phosphate-oxidase

NO

nitric oxide

ROS

reactive oxygen species

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Copyright information

© Serdi and Springer-Verlag France 2016

Authors and Affiliations

  • Thomas Nickel
    • 1
  • K. Lackermair
    • 1
  • J. Scherr
    • 2
  • A. Calatzis
    • 3
  • M. Vogeser
    • 7
  • H. Hanssen
    • 4
  • G. Waidhauser
    • 1
  • U. Schönermark
    • 5
  • H. Methe
    • 1
  • S. Horster
    • 6
  • U. Wilbert-Lampen
    • 1
  • M. Halle
    • 2
  1. 1.Department of Internal Medicine/CardiologyUniversity Hospital Grosshadern, Ludwig-Maximilians-UniversityMunichGermany
  2. 2.Department of Prevention and Sports Medicine, Klinikum rechts der IsarTechnical UniversityMunichGermany
  3. 3.Department of Transfusion Medicine and HaemostaseologyLudwig-Maximilians-UniversityMunichGermany
  4. 4.Division of Sports Medicine, Institute of Exercise and Health Sciences, Medical FacultyUniversity of BaselBaselSwitzerland
  5. 5.Department of Internal Medicine/NephrologyUniversity Hospital Grosshadern, Ludwig-Maximilians-UniversityMunichGermany
  6. 6.Department of Internal Medicine/GastroenterologyUniversity Hospital Grosshadern, Ludwig-Maximilians-UniversityMunichGermany
  7. 7.Institut for laboratory MedicineUniversity Hospital Grosshadern, Ludwig-Maximilians-UniversityMunichGermany

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