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The journal of nutrition, health & aging

, Volume 15, Issue 10, pp 883–889 | Cite as

Combined effect of antioxidant supplementation and resistance training on oxidative stress markers, muscle and body composition in an elderly population

  • F. Bobeuf
  • M. Labonte
  • I. J. Dionne
  • Abdelouahed Khalil
JNHA: Sarcopenia Frailty and Performance

Abstract

This study was aimed to examine the effect of vitamin C/E intake alone or combined with resistance training on antioxidant/pro-oxidant status, muscle strength and body composition in an elderly population. Fifty-seven men and women with a mean age of 65.6 ± 3.8 years were recruited and randomized in a double-blind fashion into four groups: control-placebo; resistance training (RT); vitamins C/E supplementation (AS); AS+RT. Oxidative stress status and metabolic and lipid profiles were determined at baseline and after six months. Fat-free mass and fat mass measured by DXA were similar at baseline for all groups. At six month, there was a significant difference among the groups as a function of vitamin E supplementation. Moreover, although there was no effect on pro-oxidative parameters, a significant effect on body composition was noted, but no difference was noted on strength gain. The combination of RT+AS had a positive effect on the plasma antioxidant profile but not on the pro-oxidant status.

Key words

Antioxidant and pro-oxidant status vitamins supplementation resistance training elderly 

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References

  1. 1.
    Sanz A, Pamplona R, Barja G. Is the mitochondrial free radical theory of aging intact? Antioxid Redox Signal 2006;8:582–599.PubMedCrossRefGoogle Scholar
  2. 2.
    Bonnefoy M, Drai J, Kostka T. [Antioxidants to slow aging, facts and perspectives]. Presse Med 2002;31:1174–1184.PubMedGoogle Scholar
  3. 3.
    Harman D. The free radical theory of aging. Antioxid Redox Signal 2003;5:557–561.PubMedCrossRefGoogle Scholar
  4. 4.
    Kaneto H, Katakami N, Kawamori D et al. Involvement of oxidative stress in the pathogenesis of diabetes. Antioxid Redox Signal 2007;9:355–366.PubMedCrossRefGoogle Scholar
  5. 5.
    Strong K, Mathers C, Leeder S, Beaglehole R. Preventing chronic diseases: how many lives can we save? Lancet 2005;366:1578–1582.PubMedCrossRefGoogle Scholar
  6. 6.
    Amuna P, Zotor FB. Epidemiological and nutrition transition in developing countries: impact on human health and development. Proc Nutr Soc 2008;67:82–90.PubMedCrossRefGoogle Scholar
  7. 7.
    Di Meo S, Venditti P. Mitochondria in exercise-induced oxidative stress. Biol Signals Recept 2001;10:125–140.PubMedCrossRefGoogle Scholar
  8. 8.
    Gomez-Cabrera MC, Domenech E, Vina J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic Biol Med 2008;44:126–131.PubMedCrossRefGoogle Scholar
  9. 9.
    Hill S, Box W, DiSilvestro RA. Moderate intensity resistance exercise, plus or minus soy intake: effects on serum lipid peroxides in young adult males. Int J Sport Nutr Exerc Metab 2004;14:125–132.PubMedGoogle Scholar
  10. 10.
    Vincent HK, Bourguignon C, Vincent KR. Resistance training lowers exerciseinduced oxidative stress and homocysteine levels in overweight and obese older adults. Obesity (Silver Spring) 2006;14:1921–1930.CrossRefGoogle Scholar
  11. 11.
    Gianni P, Jan KJ, Douglas MJ, Stuart PM, Tarnopolsky MA. Oxidative stress and the mitochondrial theory of aging in human skeletal muscle. Exp Gerontol 2004;39:1391–1400.PubMedCrossRefGoogle Scholar
  12. 12.
    Wei YH, Lu CY, Lee HC, Pang CY, Ma YS. Oxidative damage and mutation to mitochondrial DNA and age-dependent decline of mitochondrial respiratory function. Ann N Y Acad Sci 1998;854:155–170.PubMedCrossRefGoogle Scholar
  13. 13.
    Yu BP, Chung HY. Adaptive mechanisms to oxidative stress during aging. Mech Ageing Dev 2006;127:436–443.PubMedCrossRefGoogle Scholar
  14. 14.
    Goldfarb AH, McIntosh MK, Boyer BT, Fatouros J. Vitamin E effects on indexes of lipid peroxidation in muscle from DHEA-treated and exercised rats. J Appl Physiol 1994;76:1630–1635.PubMedGoogle Scholar
  15. 15.
    Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Oxidative stress and stressactivated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 2002;23:599–622.PubMedCrossRefGoogle Scholar
  16. 16.
    Nelson JL, Bernstein PS, Schmidt MC, Von Tress MS, Askew EW. Dietary modification and moderate antioxidant supplementation differentially affect serum carotenoids, antioxidant levels and markers of oxidative stress in older humans. J Nutr 2003;133:3117–3123.PubMedGoogle Scholar
  17. 17.
    Levine M, Conry-Cantilena C, Wang Y et al. Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci U S A 1996;93:3704–3709.PubMedCrossRefGoogle Scholar
  18. 18.
    Khalil A, Jay-Gerin JP, Fulop T Jr. Age-related increased susceptibility of highdensity lipoproteins (HDL) to in vitro oxidation induced by gamma-radiolysis of water. FEBS Lett 1998;435:153–158.PubMedCrossRefGoogle Scholar
  19. 19.
    Wu JH, Ward NC, Indrawan AP et al. Effects of alpha-tocopherol and mixed tocopherol supplementation on markers of oxidative stress and inflammation in type 2 diabetes. Clin Chem 2007;53:511–519.PubMedCrossRefGoogle Scholar
  20. 20.
    Gustafsson T, Kraus WE. Exercise-induced angiogenesis-related growth and transcription factors in skeletal muscle, and their modification in muscle pathology. Front Biosci 2001;6:D75–D89.PubMedCrossRefGoogle Scholar
  21. 21.
    Sacheck JM, Milbury PE, Cannon JG, Roubenoff R, Blumberg JB. Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free Radic Biol Med 2003;34:1575–1588.PubMedCrossRefGoogle Scholar
  22. 22.
    Latham NK, Anderson CS, Reid IR. Effects of vitamin D supplementation on strength, physical performance, and falls in older persons: a systematic review. J Am Geriatr Soc 2003;51:1219–1226.PubMedCrossRefGoogle Scholar
  23. 23.
    Evans WJ. Vitamin E, vitamin C, and exercise. Am J Clin Nutr 2000;72:647S–652S.PubMedGoogle Scholar
  24. 24.
    Fiatarone MA, O’Neill EF, Ryan ND et al. Exercise training and nutritional supplementation for physical frailty in very elderly people. N Engl J Med 1994;330:1769–1775.PubMedCrossRefGoogle Scholar
  25. 25.
    Dionne IJ, Melancon MO, Brochu M, Ades PA, Poelhman ET. Age-related differences in metabolic adaptations following resistance training in women. Exp Gerontol 2004;39:133–138.PubMedCrossRefGoogle Scholar
  26. 26.
    Lemmer JT, Ivey FM, Ryan AS et al. Effect of strength training on resting metabolic rate and physical activity: age and gender comparisons. Med Sci Sports Exerc 2001;33:532–541.PubMedGoogle Scholar
  27. 27.
    Canadian Society for Exercise Physiology. Reference Manual. Professional Fitness Lifestyle Consultant 2. Reference manual. Ottawa: 1996.Google Scholar
  28. 28.
    Luhrmann PM, Herbert BM, Gaster C, Neuhauser-Berthold M. Validation of a selfadministered 3-day estimated dietary record for use in the elderly. Eur J Nutr 1999;38:235–240.PubMedCrossRefGoogle Scholar
  29. 29.
    Welle S, Thornton C, Totterman S, Forbes G. Utility of creatinine excretion in bodycomposition studies of healthy men and women older than 60 y. Am J Clin Nutr 1996;63:151–156.PubMedGoogle Scholar
  30. 30.
    Bertin E, Marcus C, Ruiz JC, Eschard JP, Leutenegger M. Measurement of visceral adipose tissue by DXA combined with anthropometry in obese humans. Int J Obes Relat Metab Disord 2000;24:263–270.PubMedCrossRefGoogle Scholar
  31. 31.
    Bobeuf F, Labonte M, Khalil A, Dionne IJ. Effects of resistance training combined with antioxidant supplementation on fat-free mass and insulin sensitivity in healthy elderly subjects. Diabetes Res Clin Pract 2010;87:e1–e3.PubMedCrossRefGoogle Scholar
  32. 32.
    Bode AM, Rose RC. Analysis of water-soluble antioxidants by high-performance liquid chromatography with electrochemical detection. Methods Enzymol 1999;299:77–83.PubMedCrossRefGoogle Scholar
  33. 33.
    Miller NJ. Nonvitamin plasma antioxidants. Methods Mol Biol 1998;108:285–297.PubMedGoogle Scholar
  34. 34.
    Carter P. Ultrmicroestimation of human serum albumin: binding of the cationic dye, 5,5’-dibromo-o-cresolsulfonphthalein. Microchem J 1970;15:531–539.CrossRefGoogle Scholar
  35. 35.
    Louderback A, Measley EH, Taylor NA. A new dyedye-binding technic using bromocresol purple for determination of albumin in serum. Clin Chem 1968;14:793–794.Google Scholar
  36. 36.
    Kalckar HM. Differential spectrophotometry of purine compounds by means of specific enzymes. J Biol Chem 1947;167:429–443.PubMedGoogle Scholar
  37. 37.
    Agarwal R, Chase SD. Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples. J Chromatogr B Analyt Technol Biomed Life Sci 2002;775:121–126.PubMedCrossRefGoogle Scholar
  38. 38.
    Sung H, Nah J, Chun S, Park H, Yang SE, Min WK. In vivo antioxidant effect of green tea. Eur J Clin Nutr 2000;54:527–529.PubMedCrossRefGoogle Scholar
  39. 39.
    Groff JL, Gropper SS, Hunt SM. The Water Soluble Vitamins. Advanced Nutrition and Human Metabolism. Minneapolis: 1995:222–237.Google Scholar
  40. 40.
    Chappell LC, Seed PT, Kelly FJ et al. Vitamin C and E supplementation in women at risk of preeclampsia is associated with changes in indices of oxidative stress and placental function. Am J Obstet Gynecol 2002;187:777–784.PubMedCrossRefGoogle Scholar
  41. 41.
    Rizzo MR, Abbatecola AM, Barbieri M et al. Evidence for anti-inflammatory effects of combined administration of vitamin E and C in older persons with impaired fasting glucose: impact on insulin action. J Am Coll Nutr 2008;27:505–511.PubMedGoogle Scholar
  42. 42.
    Nelson EW, Lane H, Fabri PJ, Scott B. Demonstration of saturation kinetics in the intestinal absorption of vitamin C in man and the guinea pig. J Clin Pharmacol 1978;18:325–335.PubMedGoogle Scholar
  43. 43.
    Wilson JX. Regulation of vitamin C transport. Annu Rev Nutr 2005;25:105–125.PubMedCrossRefGoogle Scholar
  44. 44.
    Lykkesfeldt J, Christen S, Wallock LM, Chang HH, Jacob RA, Ames BN. Ascorbate is depleted by smoking and repleted by moderate supplementation: a study in male smokers and nonsmokers with matched dietary antioxidant intakes. Am J Clin Nutr 2000;71:530–536.PubMedGoogle Scholar
  45. 45.
    Morrissey PA, Sheehy PJ. Optimal nutrition: vitamin E. Proc Nutr Soc 1999;58:459–468.PubMedCrossRefGoogle Scholar
  46. 46.
    Handelman GJ, Machlin LJ, Fitch K, Weiter JJ, Dratz EA. Oral alpha-tocopherol supplements decrease plasma gamma-tocopherol levels in humans. J Nutr 1985;115:807–813.PubMedGoogle Scholar
  47. 47.
    Morinobu T, Yoshikawa S, Hamamura K, Tamai H. Measurement of vitamin E metabolites by high-performance liquid chromatography during high-dose administration of alpha-tocopherol. Eur J Clin Nutr 2003;57:410–414.PubMedCrossRefGoogle Scholar
  48. 48.
    Bates CJ, Mishra GD, Prentice A. Gamma-tocopherol as a possible marker for nutrition-related risk: results from four National Diet and Nutrition Surveys in Britain. Br J Nutr 2004;92:137–150.PubMedCrossRefGoogle Scholar
  49. 49.
    Cheeseman KH, Holley AE, Kelly FJ, Wasil M, Hughes L, Burton G. Biokinetics in humans of RRR-alpha-tocopherol: the free phenol, acetate ester, and succinate ester forms of vitamin E. Free Radic Biol Med 1995;19:591–598.PubMedCrossRefGoogle Scholar
  50. 50.
    Sfrent-Cornateanu R, Mihai C, Stoian I, Lixandru D, Bara C, Moldoveanu E. Antioxidant defense capacity in scleroderma patients. Clin Chem Lab Med 2008;46:836–841.PubMedCrossRefGoogle Scholar
  51. 51.
    Seljeskog E, Hervig T, Mansoor MA. A novel HPLC method for the measurement of thiobarbituric acid reactive substances (TBARS). A comparison with a commercially available kit. Clin Biochem 2006;39:947–954.PubMedCrossRefGoogle Scholar
  52. 52.
    Roberts LJ, Morrow JD. Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo. Free Radic Biol Med 2000;28:505–513.PubMedCrossRefGoogle Scholar
  53. 53.
    Labonté M, Bobeuf F, Bouchard DR et al. Effects of antioxidant supplements combined with resistance exercise on gains in fat-free mass in healthy elderly subjects: a pilot study. J Am Geriatr Soc 2008;56:1766–1768.PubMedCrossRefGoogle Scholar
  54. 54.
    Cartwright MJ, Tchkonia T, Kirkland JL. Aging in adipocytes: potential impact of inherent, depot-specific mechanisms. Exp Gerontol 2007;42:463–471.PubMedCrossRefGoogle Scholar
  55. 55.
    Trirogoff ML, Shintani A, Himmelfarb J, Ikizler TA. Body mass index and fat mass are the primary correlates of insulin resistance in nondiabetic stage 3–4 chronic kidney disease patients. Am J Clin Nutr 2007;86:1642–1648.PubMedGoogle Scholar
  56. 56.
    Beliaeff S, Bouchard DR, Hautier C, Brochu M, Dionne IJ. Association between muscle mass and isometric muscle strength in well-functioning older men and women. J Aging Phys Act 2008;16:484–493.PubMedGoogle Scholar
  57. 57.
    Clark BC, Manini TM. Functional consequences of sarcopenia and dynapenia in the elderly. Curr Opin Clin Nutr Metab Care 2010;13:271–276.PubMedCrossRefGoogle Scholar
  58. 58.
    Slivka D, Raue U, Hollon C, Minchev K, Trappe SW. Single Muscle Fiber Adaptations to Resistance Training in Old (>80 y) Men: Evidence for Limited Skeletal Muscle Plasticity. Am J Physiol Regul Integr Comp Physiol 2008.Google Scholar
  59. 59.
    Bautmans I, Van Puyvelde K, Mets T. Sarcopenia and functional decline: pathophysiology, prevention and therapy. Acta Clin Belg 2009;64:303–316.PubMedGoogle Scholar

Copyright information

© Serdi and Springer Verlag France 2011

Authors and Affiliations

  • F. Bobeuf
    • 1
    • 2
    • 3
  • M. Labonte
    • 1
    • 3
  • I. J. Dionne
    • 1
    • 3
  • Abdelouahed Khalil
    • 1
    • 2
    • 4
  1. 1.Research Center on AgingUniversity of SherbrookeSherbrookeCanada
  2. 2.Department of MedicineUniversity of SherbrookeSherbrookeCanada
  3. 3.Faculty of Physical Education and SportsUniversity of SherbrookeSherbrookeCanada
  4. 4.Research Centre on AgingSherbrookeCanada

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