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Combined effect of antioxidant supplementation and resistance training on oxidative stress markers, muscle and body composition in an elderly population

Abstract

This study was aimed to examine the effect of vitamin C/E intake alone or combined with resistance training on antioxidant/pro-oxidant status, muscle strength and body composition in an elderly population. Fifty-seven men and women with a mean age of 65.6 ± 3.8 years were recruited and randomized in a double-blind fashion into four groups: control-placebo; resistance training (RT); vitamins C/E supplementation (AS); AS+RT. Oxidative stress status and metabolic and lipid profiles were determined at baseline and after six months. Fat-free mass and fat mass measured by DXA were similar at baseline for all groups. At six month, there was a significant difference among the groups as a function of vitamin E supplementation. Moreover, although there was no effect on pro-oxidative parameters, a significant effect on body composition was noted, but no difference was noted on strength gain. The combination of RT+AS had a positive effect on the plasma antioxidant profile but not on the pro-oxidant status.

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References

  1. 1.

    Sanz A, Pamplona R, Barja G. Is the mitochondrial free radical theory of aging intact? Antioxid Redox Signal 2006;8:582–599.

  2. 2.

    Bonnefoy M, Drai J, Kostka T. [Antioxidants to slow aging, facts and perspectives]. Presse Med 2002;31:1174–1184.

  3. 3.

    Harman D. The free radical theory of aging. Antioxid Redox Signal 2003;5:557–561.

  4. 4.

    Kaneto H, Katakami N, Kawamori D et al. Involvement of oxidative stress in the pathogenesis of diabetes. Antioxid Redox Signal 2007;9:355–366.

  5. 5.

    Strong K, Mathers C, Leeder S, Beaglehole R. Preventing chronic diseases: how many lives can we save? Lancet 2005;366:1578–1582.

  6. 6.

    Amuna P, Zotor FB. Epidemiological and nutrition transition in developing countries: impact on human health and development. Proc Nutr Soc 2008;67:82–90.

  7. 7.

    Di Meo S, Venditti P. Mitochondria in exercise-induced oxidative stress. Biol Signals Recept 2001;10:125–140.

  8. 8.

    Gomez-Cabrera MC, Domenech E, Vina J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic Biol Med 2008;44:126–131.

  9. 9.

    Hill S, Box W, DiSilvestro RA. Moderate intensity resistance exercise, plus or minus soy intake: effects on serum lipid peroxides in young adult males. Int J Sport Nutr Exerc Metab 2004;14:125–132.

  10. 10.

    Vincent HK, Bourguignon C, Vincent KR. Resistance training lowers exerciseinduced oxidative stress and homocysteine levels in overweight and obese older adults. Obesity (Silver Spring) 2006;14:1921–1930.

  11. 11.

    Gianni P, Jan KJ, Douglas MJ, Stuart PM, Tarnopolsky MA. Oxidative stress and the mitochondrial theory of aging in human skeletal muscle. Exp Gerontol 2004;39:1391–1400.

  12. 12.

    Wei YH, Lu CY, Lee HC, Pang CY, Ma YS. Oxidative damage and mutation to mitochondrial DNA and age-dependent decline of mitochondrial respiratory function. Ann N Y Acad Sci 1998;854:155–170.

  13. 13.

    Yu BP, Chung HY. Adaptive mechanisms to oxidative stress during aging. Mech Ageing Dev 2006;127:436–443.

  14. 14.

    Goldfarb AH, McIntosh MK, Boyer BT, Fatouros J. Vitamin E effects on indexes of lipid peroxidation in muscle from DHEA-treated and exercised rats. J Appl Physiol 1994;76:1630–1635.

  15. 15.

    Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Oxidative stress and stressactivated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 2002;23:599–622.

  16. 16.

    Nelson JL, Bernstein PS, Schmidt MC, Von Tress MS, Askew EW. Dietary modification and moderate antioxidant supplementation differentially affect serum carotenoids, antioxidant levels and markers of oxidative stress in older humans. J Nutr 2003;133:3117–3123.

  17. 17.

    Levine M, Conry-Cantilena C, Wang Y et al. Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci U S A 1996;93:3704–3709.

  18. 18.

    Khalil A, Jay-Gerin JP, Fulop T Jr. Age-related increased susceptibility of highdensity lipoproteins (HDL) to in vitro oxidation induced by gamma-radiolysis of water. FEBS Lett 1998;435:153–158.

  19. 19.

    Wu JH, Ward NC, Indrawan AP et al. Effects of alpha-tocopherol and mixed tocopherol supplementation on markers of oxidative stress and inflammation in type 2 diabetes. Clin Chem 2007;53:511–519.

  20. 20.

    Gustafsson T, Kraus WE. Exercise-induced angiogenesis-related growth and transcription factors in skeletal muscle, and their modification in muscle pathology. Front Biosci 2001;6:D75–D89.

  21. 21.

    Sacheck JM, Milbury PE, Cannon JG, Roubenoff R, Blumberg JB. Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free Radic Biol Med 2003;34:1575–1588.

  22. 22.

    Latham NK, Anderson CS, Reid IR. Effects of vitamin D supplementation on strength, physical performance, and falls in older persons: a systematic review. J Am Geriatr Soc 2003;51:1219–1226.

  23. 23.

    Evans WJ. Vitamin E, vitamin C, and exercise. Am J Clin Nutr 2000;72:647S–652S.

  24. 24.

    Fiatarone MA, O’Neill EF, Ryan ND et al. Exercise training and nutritional supplementation for physical frailty in very elderly people. N Engl J Med 1994;330:1769–1775.

  25. 25.

    Dionne IJ, Melancon MO, Brochu M, Ades PA, Poelhman ET. Age-related differences in metabolic adaptations following resistance training in women. Exp Gerontol 2004;39:133–138.

  26. 26.

    Lemmer JT, Ivey FM, Ryan AS et al. Effect of strength training on resting metabolic rate and physical activity: age and gender comparisons. Med Sci Sports Exerc 2001;33:532–541.

  27. 27.

    Canadian Society for Exercise Physiology. Reference Manual. Professional Fitness Lifestyle Consultant 2. Reference manual. Ottawa: 1996.

  28. 28.

    Luhrmann PM, Herbert BM, Gaster C, Neuhauser-Berthold M. Validation of a selfadministered 3-day estimated dietary record for use in the elderly. Eur J Nutr 1999;38:235–240.

  29. 29.

    Welle S, Thornton C, Totterman S, Forbes G. Utility of creatinine excretion in bodycomposition studies of healthy men and women older than 60 y. Am J Clin Nutr 1996;63:151–156.

  30. 30.

    Bertin E, Marcus C, Ruiz JC, Eschard JP, Leutenegger M. Measurement of visceral adipose tissue by DXA combined with anthropometry in obese humans. Int J Obes Relat Metab Disord 2000;24:263–270.

  31. 31.

    Bobeuf F, Labonte M, Khalil A, Dionne IJ. Effects of resistance training combined with antioxidant supplementation on fat-free mass and insulin sensitivity in healthy elderly subjects. Diabetes Res Clin Pract 2010;87:e1–e3.

  32. 32.

    Bode AM, Rose RC. Analysis of water-soluble antioxidants by high-performance liquid chromatography with electrochemical detection. Methods Enzymol 1999;299:77–83.

  33. 33.

    Miller NJ. Nonvitamin plasma antioxidants. Methods Mol Biol 1998;108:285–297.

  34. 34.

    Carter P. Ultrmicroestimation of human serum albumin: binding of the cationic dye, 5,5’-dibromo-o-cresolsulfonphthalein. Microchem J 1970;15:531–539.

  35. 35.

    Louderback A, Measley EH, Taylor NA. A new dyedye-binding technic using bromocresol purple for determination of albumin in serum. Clin Chem 1968;14:793–794.

  36. 36.

    Kalckar HM. Differential spectrophotometry of purine compounds by means of specific enzymes. J Biol Chem 1947;167:429–443.

  37. 37.

    Agarwal R, Chase SD. Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples. J Chromatogr B Analyt Technol Biomed Life Sci 2002;775:121–126.

  38. 38.

    Sung H, Nah J, Chun S, Park H, Yang SE, Min WK. In vivo antioxidant effect of green tea. Eur J Clin Nutr 2000;54:527–529.

  39. 39.

    Groff JL, Gropper SS, Hunt SM. The Water Soluble Vitamins. Advanced Nutrition and Human Metabolism. Minneapolis: 1995:222–237.

  40. 40.

    Chappell LC, Seed PT, Kelly FJ et al. Vitamin C and E supplementation in women at risk of preeclampsia is associated with changes in indices of oxidative stress and placental function. Am J Obstet Gynecol 2002;187:777–784.

  41. 41.

    Rizzo MR, Abbatecola AM, Barbieri M et al. Evidence for anti-inflammatory effects of combined administration of vitamin E and C in older persons with impaired fasting glucose: impact on insulin action. J Am Coll Nutr 2008;27:505–511.

  42. 42.

    Nelson EW, Lane H, Fabri PJ, Scott B. Demonstration of saturation kinetics in the intestinal absorption of vitamin C in man and the guinea pig. J Clin Pharmacol 1978;18:325–335.

  43. 43.

    Wilson JX. Regulation of vitamin C transport. Annu Rev Nutr 2005;25:105–125.

  44. 44.

    Lykkesfeldt J, Christen S, Wallock LM, Chang HH, Jacob RA, Ames BN. Ascorbate is depleted by smoking and repleted by moderate supplementation: a study in male smokers and nonsmokers with matched dietary antioxidant intakes. Am J Clin Nutr 2000;71:530–536.

  45. 45.

    Morrissey PA, Sheehy PJ. Optimal nutrition: vitamin E. Proc Nutr Soc 1999;58:459–468.

  46. 46.

    Handelman GJ, Machlin LJ, Fitch K, Weiter JJ, Dratz EA. Oral alpha-tocopherol supplements decrease plasma gamma-tocopherol levels in humans. J Nutr 1985;115:807–813.

  47. 47.

    Morinobu T, Yoshikawa S, Hamamura K, Tamai H. Measurement of vitamin E metabolites by high-performance liquid chromatography during high-dose administration of alpha-tocopherol. Eur J Clin Nutr 2003;57:410–414.

  48. 48.

    Bates CJ, Mishra GD, Prentice A. Gamma-tocopherol as a possible marker for nutrition-related risk: results from four National Diet and Nutrition Surveys in Britain. Br J Nutr 2004;92:137–150.

  49. 49.

    Cheeseman KH, Holley AE, Kelly FJ, Wasil M, Hughes L, Burton G. Biokinetics in humans of RRR-alpha-tocopherol: the free phenol, acetate ester, and succinate ester forms of vitamin E. Free Radic Biol Med 1995;19:591–598.

  50. 50.

    Sfrent-Cornateanu R, Mihai C, Stoian I, Lixandru D, Bara C, Moldoveanu E. Antioxidant defense capacity in scleroderma patients. Clin Chem Lab Med 2008;46:836–841.

  51. 51.

    Seljeskog E, Hervig T, Mansoor MA. A novel HPLC method for the measurement of thiobarbituric acid reactive substances (TBARS). A comparison with a commercially available kit. Clin Biochem 2006;39:947–954.

  52. 52.

    Roberts LJ, Morrow JD. Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo. Free Radic Biol Med 2000;28:505–513.

  53. 53.

    Labonté M, Bobeuf F, Bouchard DR et al. Effects of antioxidant supplements combined with resistance exercise on gains in fat-free mass in healthy elderly subjects: a pilot study. J Am Geriatr Soc 2008;56:1766–1768.

  54. 54.

    Cartwright MJ, Tchkonia T, Kirkland JL. Aging in adipocytes: potential impact of inherent, depot-specific mechanisms. Exp Gerontol 2007;42:463–471.

  55. 55.

    Trirogoff ML, Shintani A, Himmelfarb J, Ikizler TA. Body mass index and fat mass are the primary correlates of insulin resistance in nondiabetic stage 3–4 chronic kidney disease patients. Am J Clin Nutr 2007;86:1642–1648.

  56. 56.

    Beliaeff S, Bouchard DR, Hautier C, Brochu M, Dionne IJ. Association between muscle mass and isometric muscle strength in well-functioning older men and women. J Aging Phys Act 2008;16:484–493.

  57. 57.

    Clark BC, Manini TM. Functional consequences of sarcopenia and dynapenia in the elderly. Curr Opin Clin Nutr Metab Care 2010;13:271–276.

  58. 58.

    Slivka D, Raue U, Hollon C, Minchev K, Trappe SW. Single Muscle Fiber Adaptations to Resistance Training in Old (>80 y) Men: Evidence for Limited Skeletal Muscle Plasticity. Am J Physiol Regul Integr Comp Physiol 2008.

  59. 59.

    Bautmans I, Van Puyvelde K, Mets T. Sarcopenia and functional decline: pathophysiology, prevention and therapy. Acta Clin Belg 2009;64:303–316.

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Correspondence to Abdelouahed Khalil.

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Bobeuf, F., Labonte, M., Dionne, I.J. et al. Combined effect of antioxidant supplementation and resistance training on oxidative stress markers, muscle and body composition in an elderly population. J Nutr Health Aging 15, 883–889 (2011). https://doi.org/10.1007/s12603-011-0097-2

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Key words

  • Antioxidant and pro-oxidant status
  • vitamins supplementation
  • resistance training
  • elderly