Effects of Dietary Supplementation of Recombinant Plectasin on Growth Performance, Intestinal Health and Innate Immunity Response in Broilers
- 87 Downloads
The present study was conducted to evaluate the effects of dietary supplementation of recombinant plectasin (Ple) on the growth performance, intestinal health, and serum immune parameters in broilers. A total of 288 1-day-old male broilers (Arbor Acres) were randomly allotted to four dietary treatments including the basal diet (NC) and basal diet supplemented with 10 mg enramycin/kg (PC), 100 mg Ple/kg (LPle), and 200 mg Ple/kg (HPle) diets. The results indicated Ple increased (P < 0.01) average daily gain and decreased (P ≤ 0.02) feed to gain ratio of broilers. In addition, the supplementation of Ple in the diets increased (P ≤ 0.01) duodenal lipase (day 21) and trypsin (day 42) activities compared with the NC group. Similar as the supplementation of enramycin, Ple also increased villus height and decreased crypt depth in jejunum (day 21), and thus the villus height to crypt depth ratio (P < 0.01) was increased compared to the NC group on day 42. The serum immunoglobulin M (days 21 and 42), immunoglobulin G (day 42), complement 3 (day 21), and complement 4 (days 21 and 42) were significantly increased (P ≤ 0.02) due to the supplementation of Ple and enramycin, while the concentration of malondialdehyde in jejunum was decreased (P < 0.01) in PC, LPle, and HPle groups on day 21 compared with those in the NC group. Furthermore, Ple reduced (P < 0.01) Escherichia coli and total aerobic bacteria population in ileum and cecum of birds on days 21 and 42. These results indicate that the recombinant plectasin has beneficial effects on growth performance, intestinal health, and innate immunity in broilers.
KeywordsPlectasin Broilers Growth performance Intestinal health Serum immunoglobulins
This study was supported by the National Key R&D Program of China (No. 2018YFD0500600), the Opening Foundation of Key Laboratory of Biomass Energy and Materials of Jiangsu Province (JSBEM2016013), and a Special Fund for Agro-scientific Research in the Public Interest (201403047).
Compliance with Ethical Standards
All experimental procedures were approved by the animal care and management committee of China Agriculture University. All diets were formulated to meet Aviagen broiler nutrient recommendations.
Conflict of Interest
The authors declare that they have no conflict of interest.
- 9.Wierup M (2001) The Swedish experience of the 1986 year ban of antimicrobial growth promoters, with special reference to animal health, disease prevention, productivity, and usage of antimicrobials. Microb Drug Resist 7(2):183–190. https://doi.org/10.1089/10766290152045066 CrossRefPubMedGoogle Scholar
- 16.Wen LF, He JG (2012) Dose-response effects of an antimicrobial peptide, a cecropin hybrid, on growth performance, nutrient utilisation, bacterial counts in the digesta and intestinal morphology in broilers. Brit J Nutr 108(10):1756–1763. https://doi.org/10.1017/S0007114511007240 CrossRefPubMedGoogle Scholar
- 17.Yoon JH, Ingale SL, Kim JS, Kim KH, Lee SH, Park YK, Lee SC, Kwon IK, Chae BJ (2014) Effects of dietary supplementation of synthetic antimicrobial peptide-A3 and P5 on growth performance, apparent total tract digestibility of nutrients, fecal and intestinal microflora and intestinal morphology in weanling pigs. Livest Sci 159:53–60. https://doi.org/10.1016/j.livsci.2013.10.025 CrossRefGoogle Scholar
- 19.Mygind PH, Fischer RL, Schnorr KM, Hansen MT, Sonksen CP, Ludvigsen S, Raventos D, Buskov S, Christensen B, De Maria L, Taboureau O, Yaver D, Elvig-Jorgensen SG, Sorensen MV, Christensen BE, Kjaerulff S, Frimodt-Moller N, Lehrer RI, Zasloff M, Kristensen HH (2005) Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature 437(7061):975–980. https://doi.org/10.1038/nature04051 CrossRefPubMedGoogle Scholar
- 20.Schneider T, Kruse T, Wimmer R, Wiedemann I, Sass V, Pag U, Jansen A, Nielsen AK, Mygind PH, Ravents DS, Neve S, Ravn B, Bonvin AMJJ, De Maria L, Andersen AS, Gammelgaard LK, Sahl HG, Kristensen HH (2010) Plectasin, a fungal defensin, targets the bacterial cell wall precursor lipid II. Science 328(5982):1168–1172. https://doi.org/10.1126/science.1185723 CrossRefPubMedGoogle Scholar
- 21.Hara S, Mukae H, Sakamoto N, Ishimoto H, Amenomori M, Fujita H, Ishimatsu Y, Yanagihara K, Kohno S (2008) Plectasin has antibacterial activity and no affect on cell viability or IL-8 production. Biochem Biophys Res Commun 374(4):709–713. https://doi.org/10.1016/j.bbrc.2008.07.093 CrossRefPubMedGoogle Scholar
- 22.Zhang J, Yang YL, Teng D, Tian ZG, Wang SR, Wang JH (2011) Expression of plectasin in Pichia pastoris and its characterization as a new antimicrobial peptide against Staphyloccocus and Streptococcus. Protein Expr Purif 78(2):189–196. https://doi.org/10.1016/j.pep.2011.04.014 CrossRefPubMedGoogle Scholar
- 23.Wan J, Li Y, Chen DW, Yu B, Chen G, Zheng P, Mao XB, Yu J, He J (2016) Recombinant plectasin elicits similar improvements in the performance and intestinal mucosa growth and activity in weaned pigs as an antibiotic. Anim Feed Sci Technol 211:216–226. https://doi.org/10.1016/j.anifeedsci.2015.12.003 CrossRefGoogle Scholar
- 25.Wu SD, Zhang FR, Huang ZM, Liu H, Xie CY, Zhang J, Thacker PA, Qiao SY (2012) Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Peptides 35(2):225–230. https://doi.org/10.1016/j.peptides.2012.03.030 CrossRefPubMedGoogle Scholar
- 27.Choi SC, Ingale SL, Kim JS, Park YK, Kwon IK, Chae BJ (2013) An antimicrobial peptide-A3: effects on growth performance, nutrient retention, intestinal and faecal microflora and intestinal morphology of broilers. Br Poult Sci 54(6):738–746. https://doi.org/10.1080/00071668.2013.838746 CrossRefPubMedGoogle Scholar
- 28.Choi SC, Ingale SL, Kim JS, Park YK, Kwon IK, Chae BJ (2013) Effects of dietary supplementation with an antimicrobial peptide-P5 on growth performance, nutrient retention, excreta and intestinal microflora and intestinal morphology of broilers. Anim Feed Sci Technol 185(1–2):78–84. https://doi.org/10.1016/j.anifeedsci.2013.07.005 CrossRefGoogle Scholar
- 30.Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K (2007) Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult Sci 86(2):309–317. https://doi.org/10.1093/ps/86.2.309 CrossRefPubMedGoogle Scholar
- 36.Tang ZR, Yin YL, Zhang YM, Huang RL, Sun ZH, Li TJ, Chu WY, Kong XF, Li LL, Geng MM, Tu Q (2009) Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin-lactoferrampin on performance, immune function and intestinal mucosal morphology in piglets weaned at age 21 d. Brit J Nutr 101(7):998–1005. https://doi.org/10.1017/S0007114508055633 CrossRefPubMedGoogle Scholar
- 38.Sukhotnik I, Yakirevich E, Coran AG, Siplovich L, Krausz M, Sabo E, Kramer A, Shiloni E (2002) Lipopolysaccharide endotoxemia reduces cell proliferation and decreases enterocyte apopotosis during intestinal adaptation in a rat model of short-bowel syndrome. Pediatr Surg Int 18(7):615–619. https://doi.org/10.1007/s00383-002-0862-8 CrossRefPubMedGoogle Scholar
- 41.Cao GT, Zeng XF, Chen AG, Zhou L, Zhang L, Xiao YP, Yang CM (2013) Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poult Sci 92(11):2949–2955. https://doi.org/10.3382/ps.2013-03366 CrossRefPubMedGoogle Scholar
- 42.Emami NK, Daneshmand A, Naeini SZ, Graystone EN, Broom LJ (2017) Effects of commercial organic acid blends on male broilers challenged with E-coli K88: performance, microbiology, intestinal morphology, and immune response. Poult Sci 96(9):3254–3263. https://doi.org/10.3382/ps/pex106 CrossRefPubMedGoogle Scholar
- 46.Nandi A, Banerjee G, Dan SK, Ghosh K, Ray AK (2018) Evaluation of in vivo probiotic efficiency of Bacillus amyloliquefaciens in Labeo rohita challenged by pathogenic strain of Aeromonas hydrophila MTCC 1739. Probiotics Antimicro 10(2):391–398. https://doi.org/10.1007/s12602-017-9310-x CrossRefGoogle Scholar
- 47.Ahire JJ, Mokashe NU, Chaudhari BL (2018) Effect of dietary probiotic lactobacillus helveticus on growth performance, antioxidant levels, and absorption of essential trace elements in goldfish (Carassius auratus). Probiotics Antimicro. https://doi.org/10.1007/s12602-018-9428-5