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Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits

  • Kinjal Pabari
  • Sheetal Pithva
  • Charmy Kothari
  • Ravi Kiran Purama
  • Kanthi Kiran Kondepudi
  • Bharatkumar Rajiv Manuel Vyas
  • Ramesh KothariEmail author
  • Padma AmbalamEmail author
Article

Abstract

Weissella paramesenteroides has gained a considerable attention as bacteriocin and exopolysaccharide producers. However, potential of W. paramesenteroides to utilize different prebiotics is unexplored area of research. Fruits being vectors of various probiotics, five W. paramesenteroides strains, namely, FX1, FX2, FX5, FX9, and FX12, were isolated from different fruits. They were screened and selected based on their ability to survive at pH 2.5 and in 1.0% sodium taurocholate, high cell surface hydrophobicity, mucin adhesion, bile-induced biofilm formation, antimicrobial activity (AMA) against selected enteropathogens, and prebiotic utilization ability, implicating the functional properties of these strains. In vitro safety evaluation showed that strains were susceptible to antibiotics except vancomycin and did not harbor any virulent traits such as biogenic amine production, hemolysis, and DNase production. Based on their functionality, two strains FX5 and FX9 were selected for prebiotic utilization studies by thin layer chromatography (TLC) and short-chain fatty acids (SCFAs) production by high performance liquid chromatography. TLC profile evinced the ability of these two strains to utilize low molecular weight galactooligosaccharides (GOS) and fructooligosaccharides (FOS), as only the upper low molecular weight fractions were disappeared from cell-free-supernatants (CFS). Enhanced β-galactosidase activity correlated with galactose accumulation in residual CFS of GOS displayed GOS utilization ability. Both the strains exhibited AMA against E. coli and Staph. aureus and high SCFAs production in the presence of prebiotic, suggesting their synbiotic potential. Thus, W. paramesenteroides strains FX5 and FX9 exhibit potential probiotic properties with prebiotic utilization and can be taken forward to evaluate synergistic synbiotic potential in detail.

Keywords

Weissella paramesenteroides Probiotics Prebiotics Antimicrobial activity Short-chain fatty acids 

Notes

Funding Information

This work was funded by the University Grant Commission (UGC), Government of India, under Major Research Project Scheme (MRP-Major-MICR-2013-35872). Ms. Kinjal Pabari is thankful to UGC for providing research fellowship. Authors would like to thank Center of Excellence (CoE) in Drug Discovery, Saurashtra University, Gujarat, for providing facility for HPLC analysis. Authors are grateful to Friesland Campina, the Netherlands for providing Vivinal GOS and Sweet Town Biotech, Taiwan for providing XOS.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12602_2019_9630_MOESM1_ESM.docx (20 kb)
ESM 1 (DOCX 17 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biotechnology, Christ College, Vidya NiketanSaurashtra UniversityPO, RajkotIndia
  2. 2.UGC-CAS Department of BiosciencesSaurashtra UniversityRajkotIndia
  3. 3.Government Science CollegeGandhinagarIndia
  4. 4.National Institute of Plant Genome ResearchNew DelhiIndia
  5. 5.National Agri-Food Biotechnology InstitutePunjabIndia

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