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A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects

  • R. A. YunesEmail author
  • E. U. Poluektova
  • E. V. Vasileva
  • M. V. Odorskaya
  • M. V. Marsova
  • G. I. Kovalev
  • V. N. Danilenko
Article

Abstract

Today, a number of studies conclusively show that certain bacterial strains, mainly from the genera Lactobacillus and Bifidobacterium, influence the functioning of the central nervous system, leading to changes in beahvior, nociception and the cognitive abilities of humans and animals. Such strains serve as the basis for developing probiotics with a curative potential for the central nervous system — psychobioitcs. However, the question of how to find such strains and which criteria to use for their selection remains unanswered. Some compounds produced by bacteria, such as gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter of the central nervous system, are potential mediators between bacterial cells and the host. Previously, we established that some species of Lactobacillus and Bifidobacterium are capable of producing GABA. We presumed that GABA-producing Lactobacillus and Bifidobacterium strains are great candidates to use as psychobiotics. Therefore, we selected the strains Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 as efficient GABA producers. The goal of this work was to assess the probiotic properties of the selected strains as well as their antidepressive effects in mice. We established that the ingestion of the probiotic composition based on the selected strains by BALB/c mice for 2 weeks reduced depressive-like behavior in the forced swimming test; the effect was similar to that of fluoxetine.

Keywords

Lactobacillus Bifidobacterium Gamma-aminobutiric acid Multi-strains probiotic composition Antidepressant 

Notes

Funding information

This project was funded by the state budget reserved for “Genetic technologies in biology, medicine, agricultural and environmental activities N 0112-2019-0002.”

Compliance with Ethical Standards

The experiments were approved by a Bioethics Committee of the Research Zakusov Institute of Pharmacology. The animals were handled in accordance with the guidelines set by the directive of the Ministry of Health of the Russian Federation (23.08.2010, N2708N).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12602_2019_9601_MOESM1_ESM.docx (18 kb)
ESM 1 (DOCX 17 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • R. A. Yunes
    • 1
    Email author
  • E. U. Poluektova
    • 1
  • E. V. Vasileva
    • 2
  • M. V. Odorskaya
    • 1
  • M. V. Marsova
    • 1
  • G. I. Kovalev
    • 2
  • V. N. Danilenko
    • 1
  1. 1.Vavilov Institute of General Genetics Russian Academy of SciencesMoscowRussian Federation
  2. 2.Federal State Budgetary Institution “Research Zakusov Institute of Pharmacology”MoscowRussian Federation

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