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L. plantarum, L. fermentum, and B. breve Beads Modified the Intestinal Microbiota and Alleviated the Inflammatory Response in High-Fat Diet–Fed Mice

  • Qingshen Sun
  • Xinyang Liu
  • Yanyan Zhang
  • Yong Song
  • Xiuyan Ma
  • Yue Shi
  • Xiuliang LiEmail author
Article

Abstract

This paper aims to study the effects of compound microbe-based beads on changes in the intestinal microbiota and alleviation of high-fat (HF) diet–induced inflammatory responses. Forty-eight mice were fed base chow or a high-fat diet for 4 weeks and then randomly separated into six groups: normal diet (group A), high-fat diet (group B), high-fat positive control (fed with high-fat chow plus Tetrahydrolipstatin, group C), high-fat chow plus B. breve beads (group D), high-fat chow plus L. plantarum-L. fermentum beads (group E), and high-fat chow plus L. plantarum-L. fermentum-B. breve beads (group F). The body weights were measured. The serum cytokine and lipid levels were determined by ELISA, and high-throughput sequence analysis of the fecal microbiota was conducted. Beads with cell encapsulation rates higher than 99% decreased the body weight from 50.97 ± 3.44 g in group B to 42.64 ± 2.63 g in group F at the end of the experiment (p = 0.00019). The total cholesterol content in group F was 80.14 ± 9.37 mmol/L, which was significantly lower than that in group A (96.13 ± 24.07 mmol/L) (p = 0.02765), group B (102.52 ± 12.20 mmol/L) (p = 0.00196), and group C (98.99 ± 11.32 mmol/L) (p = 0.00804). In addition, the serum IL-6 level showed no significant difference between group F and the base chow control group. The microbial cell-loaded bead intervention led to increased abundances of Bifidobacterium and Lactobacillus in mouse feces. Oral administration of three strain-based beads led to alleviation of inflammatory reactions in high-fat diet–fed mice.

Keywords

Bifidobacterium breve Lactobacillus plantarum Lactobacillus fermentum High-fat diet Inflammatory response Intestinal microbiota 

Notes

Funding Information

This work was supported by the Heilongjiang Province Natural Science Foundation of China (C2016049) and Harbin City Technology Bureau Youth Talented Person Project (RC2017QN020010).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Engineering Research Center of Agricultural Microbiology Technology, Ministry of EducationHeilongjiang UniversityHarbinChina
  2. 2.Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life SciencesHeilongjiang UniversityHarbinChina
  3. 3.Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life SciencesHeilongjiang UniversityHarbinChina

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