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Lactobacillus plantarum USM8613 Aids in Wound Healing and Suppresses Staphylococcus aureus Infection at Wound Sites

  • Jia Sin Ong
  • Todd D. Taylor
  • Cheng Chung Yong
  • Boon Yin Khoo
  • Sreenivasan Sasidharan
  • Sy Bing Choi
  • Hiroshi OhnoEmail author
  • Min Tze LiongEmail author
Article

Abstract

This study aimed to elucidate the targets and mechanisms of anti-staphylococcal effects from bioactive metabolites produced by lactic acid bacteria. We aimed to better understand the safety and efficacy of these bioactive metabolites in in vivo systems, typically at topical sites. The cell-free supernatant and protein-rich fraction from Lactobacillus plantarum USM8613 inhibited staphyloxanthin biosynthesis, reduced (p < 0.05) the cell number of Staphylococcus aureus by 106 CFU/mL and reduced biofilm thickness by 55% in S. aureus-infected porcine skins. Genome-wide analysis and gene expression analysis illustrated the production of several plantaricins, especially the plantaricins EF and JK that enhanced the anti-staphylococcal effects of L. plantarum USM8613. In vivo data using rats showed that the protein-rich fraction from L. plantarum USM8613 exerted wound healing properties via direct inhibition of S. aureus and promoted innate immunity, in which the expression of β-defensin was significantly (p < 0.05) upregulated by 3.8-fold. The protein fraction from L. plantarum USM8613 also significantly enhanced (p < 0.05) the production of cytokines and chemokines through various stages of wound recovery. Using ∆atl S. aureus, the protein-rich fraction from L. plantarum USM8613 exerted inhibitory activity via targeting the atl gene in S. aureus. Taken altogether, our present study illustrates the potential of L. plantarum USM8613 in aiding wound healing, suppressing of S. aureus infection at wound sites and promoting host innate immunity.

Keywords

Lactobacillus plantarum USM8613 Staphylococcus aureus Skin Wound healing Innate immunity 

Notes

Acknowledgements

The Δatl S. aureus strain was kindly provided by Prof. Dr. Hiroshi Ohno from the RIKEN Center for Integrative Medical Sciences (Japan).

Author Contributions

Ohno H and Liong MT designed the experiments. JS Ong, Yong CC, Khoo BY, Sasidharan S and Choi SB performed the analyses. Taylor TD, JS Ong, Yong CC, Ohno H and Liong MT wrote the manuscript.

Funding Information

This research was funded by the USM-RIKEN International Centre for Ageing Science (URICAS) grant (1001/PEKIND/870030).

Compliance with Ethical Standards

The experimental protocol for treating animals was approved and in accordance with the guidelines recommended by the USM Committee on Animal Care; reference number: USM/Animal Ethics Approval/2012/(81)(433).

Competing Interests

The authors declare that they have no competing interests.

Supplementary material

12602_2018_9505_MOESM1_ESM.docx (614 kb)
ESM 1 (DOCX 613 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jia Sin Ong
    • 1
  • Todd D. Taylor
    • 2
    • 3
  • Cheng Chung Yong
    • 1
  • Boon Yin Khoo
    • 4
  • Sreenivasan Sasidharan
    • 4
  • Sy Bing Choi
    • 5
  • Hiroshi Ohno
    • 3
    • 6
    Email author
  • Min Tze Liong
    • 1
    • 3
    Email author
  1. 1.School of Industrial TechnologyUniversiti Sains MalaysiaPenangMalaysia
  2. 2.Laboratory for Microbiome SciencesRIKEN Center for Integrative Medical SciencesYokohamaJapan
  3. 3.USM-RIKEN International Centre for Ageing Science (URICAS)Universiti Sains MalaysiaPenangMalaysia
  4. 4.Institute for Research in Molecular MedicineUniversiti Sains MalaysiaPenangMalaysia
  5. 5.School of Data SciencesPerdana UniversitySelangorMalaysia
  6. 6.Laboratory for Intestinal EcosystemRIKEN Center for Integrative Medical SciencesYokohamaJapan

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