Effects of Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 and Putative Probiotic Lactobacilli on DNA Damages in the Small Intestine of Wistar Rats In Vivo
- 4 Downloads
Double-strand breaks in the DNA of the small intestine in male Wistar rats were studied using a neutral comet assay after 7 days of feeding with a single strain probiotic formulation Narine (Vitamax-E, Armenia), containing Lactobacillus acidophilus strain Er-2317/402 Narine, and putative probiotics L. rhamnosus Vahe and L. delbrueckii IAHAHI. Type 0 (undamaged DNA), type 1 (head diameter 13.18–17.08 μm), and type 2 (14.15-μm head diameter) damaged DNA comets were studied in control and lactobacilli-fed rats using the neutral comet assay. Lactobacilli-fed rats were shown to carry only type 0 (undamaged) DNA.
Thus, the effects of probiotic Lactobacillus acidophilus strain INMIA 9602 Er 317/402 and putative probiotic lactobacilli on DNA damage in the small intestine of Wistar rats in vivo was shown, and the neutral comet assay is suggested as a potential tool for the in vivo selection of putative probiotics with DNA-protective activity.
KeywordsNeutral comet assay Small intestine Double-strand breaks of DNA Probiotic Narine L. rhamnosus L. delbrueckii
This study was supported by a grant for the International Science and Technology Center (A-2134) and the “CANDLE” Synchrotron Research Institute foundation (17A-1F010).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 8.Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B et al (2014) Expert consensus document: the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11:506–514CrossRefGoogle Scholar
- 11.Balayan M, Manvelyan A, Marutyan S et al (2015) Impact of lactobacillus acidophilus INMIA 9602 Er-2 and Escherichia coli M-17 on some clinical blood characteristics of familial Mediterranean fever disease patients from the Armenian cohort. Int J Probiotics Prebiotics 10:91–95Google Scholar
- 12.Bagherpour G, Ghasemi H, Zand B, Zarei N, Roohvand F, Ardakani EM, Azizi M, Khalaj V (2018) Oral administration of recombinant Saccharomyces boulardii expressing ovalbumin-CPE fusion protein induces antibody response in mice. Front Microbiol 9:723. https://doi.org/10.3389/fmicb.2018.00723 CrossRefGoogle Scholar
- 14.Chelule PK, Mokgatle MM, Zungu LI, Chaponda A (2014) Caregivers’ knowledge and use of fermented foods for infant and young children feeding in a rural community of odi, Gauteng province, South Africa. Health Promot Perspect 4(1):54–60. https://doi.org/10.5681/hpp.2014.007 eCollection 2014CrossRefGoogle Scholar
- 15.Suez J, Zmora N, Zilberman-Schapira G, Mor U, Dori-Bachash M, Bashiardes S, Zur M, Regev-Lehavi D, Ben-Zeev Brik R, Federici S, Horn M, Cohen Y, Moor AE, Zeevi D, Korem T, Kotler E, Harmelin A, Itzkovitz S, Maharshak N, Shibolet O, Pevsner-Fischer M, Shapiro H, Sharon I, Halpern Z, Segal E, Elinav E (2018) Post-antibiotic gut mucosal microbiome reconstitution is impaired by probiotics and improved by autologous FMT. Cell 174(6):1406–1423. https://doi.org/10.1016/j.cell.2018.08.047 CrossRefGoogle Scholar
- 17.Pepoyan A, Harutyunyan N, Grigoryan A et al (2015) Some clinical blood characteristics of patients with familial Mediterranean fever disease from an Armenian cohort. Klin Lab Diagn 60:46–47Google Scholar
- 18.Pepoyan A, Balayan M, Manvelyan A et al (2018) Probiotic Lactobacillus acidophilus strain INMIA 9602 Er 317/402 administration reduces the numbers of Candida albicans and abundance of enterobacteria in the gut microbiota of familial Mediterranean fever patients. Front Immunol 9:1426. https://doi.org/10.3389/fimmu.2018.01426 CrossRefGoogle Scholar
- 19.Pepoyan AZ, Balayan MH, Manvelyan AM, Mamikonyan V, Isajanyan M, Tsaturyan VV, Kamiya S, Netrebov V, Chikindas ML (2017) Lactobacillus acidophilus INMIA 9602 Er-2 strain 317/402 probiotic regulates growth of commensal Escherichia coli in gut microbiota of familial Mediterranean fever disease subjects. Lett Appl Microbiol 64:254–260. https://doi.org/10.1111/lam.12722 CrossRefGoogle Scholar
- 21.Jayakumar S, Bhilwade HN, Pandey BN, Sandur SK, Chaubey RC (2012) The potential value of the neutral comet assay and the expression of genes associated with DNA damage in assessing the radiosensitivity of tumor cells. Mutat Res 748(1–2):52–59. https://doi.org/10.1016/j.mrgentox.2012.06.008 CrossRefGoogle Scholar
- 25.Cortes-Gutierrez EI, Hernandez-Garza F, Garcia Perez JO et al (2012) Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques. J Biomed Biotechnol 2012:385245–385247. https://doi.org/10.1155/2012/385245 CrossRefGoogle Scholar
- 26.Negritto MC (2010) Repairing double-strand DNA breaks. Nature Education 3(9):26Google Scholar
- 28.Bok E, Mazurek J, Myc A, Stosik M, Wojciech M, Baldy-Chudzik K (2018) Comparison of commensal Escherichia coli isolates from adults and young children in Lubuskie Province, Poland: virulence potential, phylogeny and antimicrobial resistance. Int J Environ Res Public Health 15(4):617. https://doi.org/10.3390/ijerph15040617 CrossRefGoogle Scholar
- 29.Sengupta P (2013) The laboratory rat relating its age with humans. Int J Prev Med 4(6):624–630Google Scholar
- 38.Mkrtchyan H, Gibbons S, Heidelberger S, Zloh M, Limaki HK (2010) Purification, characterization and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine. Int J Antimicrob Agents 35(3):255–260. https://doi.org/10.1016/j.ijantimicag.2009.11.017 CrossRefGoogle Scholar