Effect of Probiotics Bacillus coagulans and Lactobacillus plantarum on Lipid Profile and Feces Bacteria of Rats Fed Cholesterol-Enriched Diet
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The aim of this study was to investigate the effect of Lactobacillus plantarum and Bacillus coagulans on serum lipid profile and lowering potential of probiotic in hypercholesterolemic rats. Twenty-eight male Wistar rats were divided into four groups as follows: (1) control group, fed standard commercial diet; (2) HC group, fed high-cholesterol diet; (3) HC + LP group, fed high-cholesterol diet and gavaging of L. plantarum; and (4) HC + BC group fed high-cholesterol diet and gavaging of B. coagulans. After 28 and 50 days, serum lipid profile; serum ALT and AST; the body and organ weights; fecal total count; Enterobacteriaceae, L. plantarum, and B. coagulans counts; and blood glucose tolerance were measured. We observed that levels of triglyceride, cholesterol, LDL, VLDL, and atherogenic index in serum were significantly lower in the HC + probiotic groups. Also, serum ALT and AST were significantly decreased in probiotic-treated groups. In addition, we found that feeding of a high-cholesterol diet for 50 days produced significant increases in the body weight, in addition to the fact that the administration of L. plantarum and B. coagulans has considerably reduced the body weight gain. B. coagulans and L. plantarum can survive passing through the upper-gastrointestinal tract after oral feeding to the rats and colonized in their colon. These bacteria could be exploited as a potential biotherapeutic remedy to reduce TC, TG, LDL, VLDL, and atherogenic index in hypercholesterolemic condition.
KeywordsProbiotics Hypercholesterolemia Lipid profile Bacillus coagulans Lactobacillus plantarum
We would like to thank Miss M. Aghazi and Mr. G. Niknia for their technical assistance.
This research was financially supported by Shiraz University.
Compliance with Ethical Standards
The authors declare that they have no conflict of interest.
This work was approved by the Ethics Committee of the School of Veterinary Medicine, Shiraz University, Shiraz, Iran (ethical approved number 1392/909342).
- 2.Sudha MR, Chauhan P, Dixit K, Babu S, Jami K (2009) Probiotics as complementary therapy for hypercholesterolemia. Biol Med 1(4):1–13Google Scholar
- 10.Russell BL, Jelley SAA, Yousten AA (1989) Selective medium for mosquito pathogenic strains of Bacillus sphaericus 2362. J Appl Environ Microbiol 55:294–297Google Scholar
- 15.Haroun BM, Refaat BM, El-Waseif AA, El- Menoufy HA, Amin HA (2013) Hypolipidemic activity of the probiotic Lactobacillus plantarum NRRL B-4496 and their prebiotic exopolysaccharide in vitro and in vivo. JASR 9(1):1015–1020Google Scholar
- 18.An HM, Park SY, Lee DK, Kim RJ, Cha MK, Lee SW, Lim HT, Kim KJ, Ha NJ (2011) Anti-obesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids Health Dis 10(116):1–8Google Scholar
- 19.Ranasinghe JGS, Silva SSP, Herath N (2013) Changes of serum lipid and proteins during probiotic feeding and its exposure. IJSRP 3:1–5Google Scholar
- 21.Sirilun S, Chaiyasut C, Kantachote D, Luxananil P (2010) Characterisation of non-human origin probiotic Lactobacillus plantarum with cholesterol-lowering property. Afr J Microbiol Res 4:994–1000Google Scholar
- 23.El Mahmoudy AM, Abdel-Fattah FA, Abd El-Mageid AD, Gheith IM (2014) Effect of the growth promotant mannan-oligosaccharide on the lipogram and organ function profile in hyperlipidemic albino rats. Am J Phytomedicine Clin Ther 2(3):334–347Google Scholar
- 26.El-Shafie HA, Yahia NI, Ali HA, Khalil FA, El-Kady EM, Moustafa YA (2009) Hypocholesterolemic action of Lactobacillus plantarum NRRL-B-4524 and Lactobacillus paracasei in mice with hypercholesterolemia induced by diet. Aust J Basic Appl Sci 3(1):218–228Google Scholar