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Probiotics and Antimicrobial Proteins

, Volume 11, Issue 4, pp 1163–1171 | Cite as

Effect of Probiotics Bacillus coagulans and Lactobacillus plantarum on Lipid Profile and Feces Bacteria of Rats Fed Cholesterol-Enriched Diet

  • Ladan Aminlari
  • Seyed Shahram ShekarforoushEmail author
  • Saeid Hosseinzadeh
  • Saeed Nazifi
  • Javad Sajedianfard
  • Mohammad Hadi Eskandari
Article

Abstract

The aim of this study was to investigate the effect of Lactobacillus plantarum and Bacillus coagulans on serum lipid profile and lowering potential of probiotic in hypercholesterolemic rats. Twenty-eight male Wistar rats were divided into four groups as follows: (1) control group, fed standard commercial diet; (2) HC group, fed high-cholesterol diet; (3) HC + LP group, fed high-cholesterol diet and gavaging of L. plantarum; and (4) HC + BC group fed high-cholesterol diet and gavaging of B. coagulans. After 28 and 50 days, serum lipid profile; serum ALT and AST; the body and organ weights; fecal total count; Enterobacteriaceae, L. plantarum, and B. coagulans counts; and blood glucose tolerance were measured. We observed that levels of triglyceride, cholesterol, LDL, VLDL, and atherogenic index in serum were significantly lower in the HC + probiotic groups. Also, serum ALT and AST were significantly decreased in probiotic-treated groups. In addition, we found that feeding of a high-cholesterol diet for 50 days produced significant increases in the body weight, in addition to the fact that the administration of L. plantarum and B. coagulans has considerably reduced the body weight gain. B. coagulans and L. plantarum can survive passing through the upper-gastrointestinal tract after oral feeding to the rats and colonized in their colon. These bacteria could be exploited as a potential biotherapeutic remedy to reduce TC, TG, LDL, VLDL, and atherogenic index in hypercholesterolemic condition.

Keywords

Probiotics Hypercholesterolemia Lipid profile Bacillus coagulans Lactobacillus plantarum 

Notes

Acknowledgements

We would like to thank Miss M. Aghazi and Mr. G. Niknia for their technical assistance.

Funding

This research was financially supported by Shiraz University.

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no conflict of interest.

Ethical Approval

This work was approved by the Ethics Committee of the School of Veterinary Medicine, Shiraz University, Shiraz, Iran (ethical approved number 1392/909342).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ladan Aminlari
    • 1
  • Seyed Shahram Shekarforoush
    • 1
    Email author
  • Saeid Hosseinzadeh
    • 1
  • Saeed Nazifi
    • 2
  • Javad Sajedianfard
    • 3
  • Mohammad Hadi Eskandari
    • 4
  1. 1.Department of Food Hygiene and Public Health, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Clinical Study, School of Veterinary MedicineShiraz UniversityShirazIran
  3. 3.Department of Physiology, School of Veterinary MedicineShiraz UniversityShirazIran
  4. 4.Department of Food Science and Technology, College of AgricultureShiraz UniversityShirazIran

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