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Effects of Lactobacillus acidophilus and Bifidobacterium bifidum Probiotics on the Expression of MicroRNAs 135b, 26b, 18a and 155, and Their Involving Genes in Mice Colon Cancer

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Abstract

A wide range of sources supports that the link between diet and colorectal cancer may be due to an imbalance of the intestinal microflora. In this case, it seems that the probiotics may have a possible molecular mechanism via microRNAs (miRNAs). The present study is aimed to evaluate the effects of Lactobacillus acidophilus and Bifidobacterium bifidum probiotics on the expression of miRNAs 135b, 26b, 18a, and 155 and their target genes, including APC, PTEN, KRAS, and PU.1 in mouse azoxymethane (AOM)-induced colon cancer. Thirty-eight male BALB/c mice were randomly divided into four groups: the control, AOM, Lactobacillus acidophilus, and Bifidobacterium bifidum to deliberate the effects of the probiotics on the miRNAs and their target genes. Except for the control group, the rest groups were weekly given AOM (15 mg/kg, s.c) in three consecutive weeks to induce mouse colon cancer. The animals were given 1.5 g powders of L. acidophilus (1 × 109 cfu/g) and B. bifidum (1 × 109 cfu/g) in 30 cc drinking water in the related groups for 5 months. At the end of the study, the animals were sacrificed and their blood and colon samples were removed for the molecular analyses. The results showed that the expression of the miR-135b, miR-155, and KRAS was increased in the AOM group compared to the control group in both the plasma and the colon tissue samples, and the consumption of the probiotics decreased their expression. Moreover, the miR-26b, miR-18a, APC, PU.1, and PTEN expressions were decreased in the AOM group compared to the control group and the consumption of the probiotics increased their expressions. It seems that Lactobacillus acidophilus and Bifidobacterium bifidum though increasing the expression of the tumor suppressor miRNAs and their target genes and decreasing the oncogenes can improve colon cancer treatment.

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Funding

This study was supported by Iran University of Medical Sciences (Grant No. 24067).

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Author notes

  1. Ali Mohammad Alizadeh and Shahram Agah contributed equally to this work.

Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

    Zahra Heydari & Mahdi Rahaie

  2. Cancer Research Center, Tehran University of Medical Sciences, Tehran, 1419733141, Iran

    Ali Mohammad Alizadeh & Solmaz Khalighfard

  3. Cancer Biology Research Center, Tehran University of Medical Science, Tehran, Iran

    Ali Mohammad Alizadeh

  4. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran

    Shahram Agah

  5. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Solmaz Khalighfard

  6. Research and Development Department, Zist Takhmir Company, Tehran, Iran

    Sahar Bahmani

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  1. Zahra Heydari
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Correspondence to Ali Mohammad Alizadeh or Shahram Agah.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Heydari, Z., Rahaie, M., Alizadeh, A.M. et al. Effects of Lactobacillus acidophilus and Bifidobacterium bifidum Probiotics on the Expression of MicroRNAs 135b, 26b, 18a and 155, and Their Involving Genes in Mice Colon Cancer. Probiotics & Antimicro. Prot. 11, 1155–1162 (2019). https://doi.org/10.1007/s12602-018-9478-8

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