Evaluation of the Probiotic Potential of Saccharomyces cerevisiae Strain (C41) Isolated from Tibicos by In Vitro Studies
Artisanal fermented beverages have been associated with beneficial effects for a long time. In Mexico, there are a wide variety of artisanal fermented beverages such as Tepache, where the fermentation is initiated by the addition of grains of a polysaccharide-containing biofilm matrix formed by a symbiotic association of bacteria and yeasts known as “Tibicos.” These microorganisms can be responsible for the beneficial effect associated with fermented beverages inoculated with Tibicos. The probiotic potential of microorganisms has been widely studied, mainly in lactic acid bacteria, while despite the wide distribution of yeasts, these have not been so studied. Therefore, the aim of this study was to evaluate in vitro the probiotic potential properties of a yeast isolated from Tibicos. For this, the yeast was identified by molecular techniques as Saccharomyces cerevisiae, which showed a good resistance to pH 2.0, bile salts and in vitro digestion. The results also showed a good ability to form cellular aggregates as a result of having a hydrophobic surface. In addition, it can be considered as safe since it does not show hemolytic activity and is sensitive to nystatin. Additionally, the yeast presented an excellent antioxidant capacity to reduce the DPPH radical. The S. cerevisiae strain C41 isolated from Tibicos was successfully compared by means of in vitro tests with the only recognized probiotic yeast, S. boulardii. These findings point Saccharomyces cerevisiae C41 as a potentially probiotic yeast; nevertheless, it is necessary to consider further in vitro and in vivo studies that establish the benefits that this yeast could provide.
KeywordsProbiotic yeast Saccharomyces cerevisiae Tibicos Fermented beverages
This research was supported by the National Council for Science and Technology from Mexico (260107).
Compliance with Ethical Standards
Conflict of Interests
The authors declare that they have no conflict of interest.
This study does not contain any studies with human participants or animals performed by any of the authors.
For this type of study, formal consent is not required, because it does not contain studies with human participants.
- 1.Thombre R, Joshi S (2017) Industrial production and applications of yeast and yeast products. In: Thangadurai D, Sangeetha J (eds) Industrial biotechnology: sustainable production and bioresource utilization. Apple Academic Press, New Jersey, pp 59–75Google Scholar
- 4.Rubio M, Lappe P, Wacher C, Ulloa M (1993) Estudio microbiano y químico de la fermentación de soluciones de piloncillo inoculadas con Tibicos. Rev Lat Am Microbiol 35(1):19–31Google Scholar
- 7.Akabanda F, Owusu-Kwarteng J, Tano-Debrah K, Glover RLK, Nielsen DS, Jespersen L (2013) Taxonomic and molecular characterization of lactic acid bacteria and yeasts in Nunu, a Ghanaian fermented milk product. Food Microbiol 34:277–283. https://doi.org/10.1016/j.fm.2012.09.025 CrossRefPubMedPubMedCentralGoogle Scholar
- 9.Generoso S, Viana M, Santos R, Martins F, Machado J, Arantes R, Nicoli J, Correia M, Cardoso VN (2010) Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model. Arch Microbiol 192:477–484. https://doi.org/10.1007/s00203-010-0574-8 CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Gedek BR (1991) Regulierung der darmflora über die nahrung. Zbl Hyg Umweltmed 191:277–301Google Scholar
- 11.Joint FAO/WHO Working Group (2002) Guidelines for the evaluation of probiotics in food. World Health Organization, LondonGoogle Scholar
- 16.Argyri AA, Zoumpopoulou G, Karatzas KAG, Tsakalidou E, Nychas GJE, Panagou EZ, Tassou CC (2013) Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiol 33(2):282–291. https://doi.org/10.1016/j.fm.2012.10.005 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Pedersen LL, Owusu-Kwarteng J, Thorsen L, Jespersen L (2012) Biodiversity and probiotic potential of yeasts isolated from Fura, a West African spontaneously fermented cereal. Int J Food Microbiol 159(2):144–151. https://doi.org/10.1016/j.ijfoodmicro.2012.08.016 CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Nwanyanwu CE, Alisi CS, Nweke CO, Orji JC (2012) Cell surface properties of phenol-utilizing bacteria isolated from petroleum refinery wastewater. J Res Biol 2:383–391Google Scholar
- 32.Dixit K, Gandhi DN (2006) Safety of probiotic yeasts. Available from: http://wwwdairyscienceinfo/indexphp/probiotics/105-biotherapeutic-probiotic-yeasthtml?showall=& start=4/Accessed 12 May 2017
- 33.Elmer GW, Moyer KA, Vega R, Surawicz CM, Collier AC, Hooton TM, McFarland LV (1995) Evaluation of Saccharomyces boulardii for patients with HIV-related chronic diarrhea and in healthy volunteers receiving antifungals. Microb Ther 25:23–31Google Scholar
- 35.De Rosa CP, Paglia A, Sasso L, Di Palma V, Chiariello M (2010) Reactive oxygen species and antioxidants in the pathophysiology of cardiovascular disease: does the actual knowledge justify a clinical approach? Curr Vasc Pharmacol 8(2):259–275. https://doi.org/10.2174/157016110790887009 CrossRefPubMedPubMedCentralGoogle Scholar
- 39.Balasubramanian K, Ragunathan R (2012) Study of antioxidant and anticancer activity of natural sources. J Nat Prod Plant Res 2:192–197Google Scholar
- 44.Annan NT, Poll L, Sefa Dedeh S, Plahar WA, Jakobsen M (2003) Influence of starter culture combinations of Lactobacillus fermentum, Saccharomyces cerevisiae and Candida krusei on aroma in Ghanaian maize dough fermentation. Eur Food Res Technol 216:377–384. https://doi.org/10.1007/s00217-003-0692-5 CrossRefGoogle Scholar