Growth Behavior and Fatty Acid Production of Probiotics, Pediococcus acidilactici and Lactococcus lactis, at Different Concentrations of Fructooligosaccharide: Studies Validating Clinical Efficacy of Selected Synbiotics on Growth Performance of Caspian Roach (Rutilus frisii kutum) Fry
Growth behavior and production of short-chain fatty acid (SCFA) of two probiotics, Pediococcus acidilactici and Lactococcus lactis, each at 107 cfu/g (P1, L1) and 1010 cfu/g (P2, L2) at different concentrations of fructooligosaccharide (FOS) [0.5% (F1), 1% (F2), and 2% (F3)] were assessed in vitro. The time to reach the maximum growth of the probiotics in all 12 treatments was between 8 to 10 h, with the highest and the lowest growth rates obtained in F1L1P1 (0.34 ± 0.02 OD) and F3L1P1 (0.31 ± 0.05 OD) treatments, respectively. The shortest and the longest generation times were seen in F1L1P1 (112 ± 1.5 min) and F2L1P1 (231 ± 0.5 min) treatments, respectively. The highest and the lowest levels of SCFA production were found in F1L1P1 (17.94 ± 0.74 mg/L) and F3L1P1 (12.98 ± 0.85 mg/L) treatments, respectively. The three synbiotics with the highest SCFA production were then fed to Caspian roach (Rutilus frisii kutum) fry weighing 0.75 ± 0.02 g at 28 °C for 60 days, to assess growth performance and enzymatic activity. The best growth performance in terms of weight gain (WG), protein efficiency ratio (PER), net protein utilization (NPU), and food conversion ratio (FCR) were seen with F1L1P1. In addition, the highest activity levels of the digestive enzymes chymotrypsin, lipase, and amylase were obtained with F1P1L1. The correlation of these in vitro and in vivo data demonstrated that oral application of these two probiotics each at 107 cfu/g feed plus 0.5% FOS can improve growth and gut enzyme activity of Caspian roach fry.
KeywordsRutilus frisii Lipase Amylase Chymotrypsin P. acidilactici L. Lactis Fatty acid Synbiotic
This research work was supported by the University of Tehran and the Centre for Sustainable Aquatic Ecosystems, Murdoch University, and so, their support is fully acknowledged.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest. This work was performed according to the guidelines of the Ethics Committee for Research on Animals at the University of Tehran.
- 4.Geraylou Z, Souffreau C, Rurangwa E, De Meester L, Courtin CM, Delcour JA, Buyse J, Ollevier F (2013) Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut microbiota of juvenile Siberian sturgeon (Acipenser baerii). Fish Shellfish Immunol 35:766–775CrossRefGoogle Scholar
- 5.Merrifield D, Bradley G, Harper G, Baker R, Munn C, Davies S (2011) Assessment of the effects of vegetative and lyophilized Pediococcus acidilactici on growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum). Aquac Nutr 17:73–79CrossRefGoogle Scholar
- 7.Ringø E, Dimitroglou A, Hoseinifar SH, Davies SJ (2014) Prebiotics in finfish: an update. In: Merrifield DL, Ringø E (eds) Aquaculture nutrition: gut health, probiotics and prebiotics, 1st edn. Wiley-Blackwell, Oxford, pp 360–400Google Scholar
- 14.Zhang Q, Tan B, Mai K, Zhang W, Ma H, Ai Q, Wang X, Liufu Z (2013) Dietary administration of Bacillus (B. licheniformis and B. subtilis) and isomaltooligosaccharide influences the intestinal microflora, immunological parameters and resistance against Vibrio alginolyticus in shrimp, Penaeus japonicas (Decapoda: Penaeidae). Aquac Res 42:943–952CrossRefGoogle Scholar
- 15.Ye JK, Wang F, Li Y (2011) Single or combined effects of fructo- and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichthys olivaceus. Aquac Nutr 17:902–911CrossRefGoogle Scholar
- 18.Rurangwa E, Laranja JL, Van Houdt R, Delaedt Y, Geraylou Z, Van de Wiele T, Van Loo J, Van Craeyveld V, Courtin CM, Delcour JA (2009) Selected non-digestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono cultures in vitro. J Appl Microbiol 106:932–940CrossRefGoogle Scholar
- 19.AOAC (Association of Official Analytical Chemists) (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Inc., ArlingtonGoogle Scholar
- 22.Worthington K, Worthington V (1993) Worthington enzyme manual: enzymes and related biochemical, 3rd edn. Worthington Biochemical Corporation, Freehold, LakewoodGoogle Scholar
- 23.Pak D, Muthaiyan A, Story RS, O'Bryan CA, Lee SO, Crandall PG, Ricke SC (2013) Fermentative capacity of three strains of Lactobacillus using different sources of carbohydrates: in vitro evaluation of synbiotic effects, resistance and tolerance to bile and gastric juices. J Food Res 2:158–167CrossRefGoogle Scholar
- 24.Hoseinifar SH, Mirvaghefi A, Amoozegar MA, Merrifield DL, Ringø E (2017) In vitro selection of a synbiotic and in vivo evaluation on intestinal microbiota, performance and physiological response of rainbow trout (Oncorhynchus mykiss) fingerlings. Aquac Nutr 23:111–118. https://doi.org/10.1111/anu.12373 CrossRefGoogle Scholar
- 27.Ogué-Bon E, Khoo C, McCartney AL, Gibson GL, Rastall R (2010) In vitro effect of synbiotic fermentation on the canine fecal microbiota. FEMS Microbiol Ecol 73:587–600Google Scholar
- 30.Marx SP, Winkler S, Hartmeier W (2000) Metabolization of L-(2, 6)-linked fructose-oligosaccharides by different bifidobacteria. FEMS Microbiol Lett 182:163–169Google Scholar
- 32.Soleimani N, Hoseinifar SH, Merrifield DL, Barati M, Hassan Abadi Z (2012) Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 32:316–321CrossRefGoogle Scholar
- 34.Azimirad M, Meshkini S, Ahmadifard N, Hoseinifar SH (2016) Effects of feeding with synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (Pterophyllum scalare). Fish Shellfish Immunol 54:516–522. https://doi.org/10.1016/j.fsi.2016.05.001. CrossRefGoogle Scholar
- 37.Khosravi-Bakhtiarvandi N, Abedian-Kenari AM (2015) Changes of digestive enzymes activity in Caspian Kutum (Rutilus frisii kutum) during larval developmental stages. Iran J Fish Sci 14:158–175Google Scholar
- 38.Sunde J, Eiane SA, Rustad A, Jensen HB, Opstvedt J, Nygård E, Rungruangsak-Torrissen K (2004) Effect of fish feed processing conditions on digestive protease activities, free amino acid pools, feed conversion efficiency and growth in Atlantic salmon (Salmo salar L.). Aquac Nutr 10:261–277. https://doi.org/10.1111/j.1365-2095.2004.00300.x CrossRefGoogle Scholar