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Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 973–980 | Cite as

Pseudomonas aeruginosa FARP72 Offers Protection Against Aeromonas hydrophila Infection in Labeo rohita

  • Farhana HoqueEmail author
  • T. Jawahar Abraham
  • T. S. Nagesh
  • Dibyendu Kamilya
Article

Abstract

Use of probiotics as the biocontrol agent for disease prevention in aquaculture is gaining importance as an alternative to the indiscriminate use of antibiotics and other chemotherapeutics. In view of this trend, the probiotic properties of a potent antagonistic bacterium, Pseudomonas aeruginosa FARP72, was characterized in terms of safety, antagonistic activities, in vitro immunomodulation, and in vivo disease resistance. Immunomodulatory activity was ascertained by measuring the production of intracellular superoxide anion, nitric oxide, total leukocyte peroxidase content, and the leukocyte proliferation in head kidney leukocytes. The bacterium isolated from the skin mucus of freshwater catfish Clarias batrachus was harmless to Labeo rohita. It showed inhibitory activity against Aeromonas caviae, A. hydrophila, Edwardsiella tarda, Pseudomonas putida, and Streptococcus agalactiae as revealed by cross and parallel streaking methods. Significantly higher superoxide anion and nitric oxide production, peroxidase content, and proliferative responses of leucocytes delineated the strains’ ability to interact with immune cells to activate the immune system in vitro. Significant growth inhibition of A. hydrophila from 1.55 × 105 CFU/mL was observed when co-cultured with P. aeruginosa FARP72 in phosphate-buffered saline (PBS) at levels ranging from 2.61 × 107 to 2.61 × 109 CFU/mL in 10 days. P. aeruginosa FARP72 increased the survival rate of rohu fingerlings against pathogenic A. hydrophila challenge in biocontrol study in vivo as determined by cohabitation challenge. These results suggest that P. aeruginosa FARP72 is a potential probiotic strain and can be used in aquaculture to improve the health status and disease resistance of fish.

Keywords

Labeo rohita Aeromonas hydrophila Pseudomonas aeruginosa Biocontrol Probiotics 

Notes

Acknowledgements

The authors thank the Vice-Chancellor, West Bengal University of Animal and Fishery Sciences, Kolkata for providing necessary infrastructure facility to carry out the work.

Compliance with Ethical Standards

Ethical Statement

All authors of this paper have read and approved the final version submitted. The contents of this manuscript have not been copyrighted or published previously.

1. The contents of this manuscript are not now under consideration for publication elsewhere.

2. The contents of this manuscript will not be copyrighted, submitted, or published elsewhere, while acceptance by the Journal is under consideration.

3. Use of laboratory animals (fish) in the present study has complied with the guidelines and policies of the ethical committee of the Institute.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Farhana Hoque
    • 1
    • 2
    Email author
  • T. Jawahar Abraham
    • 1
  • T. S. Nagesh
    • 3
  • Dibyendu Kamilya
    • 4
  1. 1.Department of Aquatic Animal Health, Faculty of Fishery SciencesWest Bengal University of Animal and Fishery SciencesKolkataIndia
  2. 2.Regional Research CentreICAR-Central Institute of Freshwater AquacultureKolkataIndia
  3. 3.Department of Fisheries Resource Management, Faculty of Fishery SciencesWest Bengal University of Animal and Fishery SciencesKolkataIndia
  4. 4.Department of Aquatic Health and Environment, College of FisheriesCentral Agricultural UniversityAgartalaIndia

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