Exploring the Probiotic and Compound Feed Fermentative Applications of Lactobacillus plantarum SK1305 Isolated from Korean Green Chili Pickled Pepper
Herein, we explore the probiotic potentials and soybean meal (SBM) compound feed fermentative applications of Lactobacillus plantarum SK1305 strain isolated previously from Korean green chili pickled pepper (gochu-jangajji). The isolate exhibited higher acid (pH 2.5) and bile tolerance (0.3%, w/v) up to 2 h and 4 h, respectively. The cell-free culture supernatant (CFCS) displayed a broad spectrum antibacterial activities against various pathogens, which may be ascribed to high lactic acid production (L-form, 86.8 ± 0.8 mM and D-form, 44.8 ± 0.2 mM). Further, the strain displayed high cell-surface hydrophobicity (92.7 ± 1.0%), coupled with low auto-aggregation (23.6 ± 4.4%) but relatively higher co-aggregation properties with C. perfringens (49.6 ± 0.6%) as well as H2O2 (1.0 mM) resistant property. Additionally, the isolate displayed significant DPPH free radical scavenging activity (55.2 ± 0.6%) and superoxide reducing ability in MAC-T cells. Considering safety, the isolate has no transmissible antibiotic resistant genes and harmful enzymes as well as non-hemolytic activities. Ushered by these appreciable probiotic properties, the isolate was used for solid state fermentation (SSF) of SBM compound feed. Notably, we observed a higher strain adaptability (> 1010 CFU/g) following the production of L- (> 6.0 ± 0.0 mM) and D-form (> 5.2 ± 0.3 mM) lactic acid during fermentation for 8 h. The methanolic extracts of fermented feed displayed high antibacterial and antioxidant activities, affirming the potential functional activities of fermented compound feeds. Therefore, L. plantarum SK1305 may act as a worthy inoculum toward fermentation of feed with enhanced nutritional properties.
KeywordsAntibacterial activity Antioxidant activity Lactic acid Lactobacillus plantarum SK1305 Solid state fermentation
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio Industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (No.312058–03) and Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ010906), Rural Development Administration, Republic of Korea.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest.
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