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Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 801–812 | Cite as

Exploring the Probiotic and Compound Feed Fermentative Applications of Lactobacillus plantarum SK1305 Isolated from Korean Green Chili Pickled Pepper

  • Kai-Min Niu
  • Damini Kothari
  • Sang-Buem Cho
  • Sung-Gu Han
  • In-Geun Song
  • Sam-Churl Kim
  • Soo-Ki KimEmail author
Article

Abstract

Herein, we explore the probiotic potentials and soybean meal (SBM) compound feed fermentative applications of Lactobacillus plantarum SK1305 strain isolated previously from Korean green chili pickled pepper (gochu-jangajji). The isolate exhibited higher acid (pH 2.5) and bile tolerance (0.3%, w/v) up to 2 h and 4 h, respectively. The cell-free culture supernatant (CFCS) displayed a broad spectrum antibacterial activities against various pathogens, which may be ascribed to high lactic acid production (L-form, 86.8 ± 0.8 mM and D-form, 44.8 ± 0.2 mM). Further, the strain displayed high cell-surface hydrophobicity (92.7 ± 1.0%), coupled with low auto-aggregation (23.6 ± 4.4%) but relatively higher co-aggregation properties with C. perfringens (49.6 ± 0.6%) as well as H2O2 (1.0 mM) resistant property. Additionally, the isolate displayed significant DPPH free radical scavenging activity (55.2 ± 0.6%) and superoxide reducing ability in MAC-T cells. Considering safety, the isolate has no transmissible antibiotic resistant genes and harmful enzymes as well as non-hemolytic activities. Ushered by these appreciable probiotic properties, the isolate was used for solid state fermentation (SSF) of SBM compound feed. Notably, we observed a higher strain adaptability (> 1010 CFU/g) following the production of L- (> 6.0 ± 0.0 mM) and D-form (> 5.2 ± 0.3 mM) lactic acid during fermentation for 8 h. The methanolic extracts of fermented feed displayed high antibacterial and antioxidant activities, affirming the potential functional activities of fermented compound feeds. Therefore, L. plantarum SK1305 may act as a worthy inoculum toward fermentation of feed with enhanced nutritional properties.

Keywords

Antibacterial activity Antioxidant activity Lactic acid Lactobacillus plantarum SK1305 Solid state fermentation 

Notes

Funding

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio Industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (No.312058–03) and Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ010906), Rural Development Administration, Republic of Korea.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Kai-Min Niu
    • 1
  • Damini Kothari
    • 1
  • Sang-Buem Cho
    • 2
  • Sung-Gu Han
    • 3
  • In-Geun Song
    • 4
  • Sam-Churl Kim
    • 5
  • Soo-Ki Kim
    • 1
    Email author
  1. 1.Department of Animal Science and TechnologyKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Animal ScienceChonbuk National UniversityJeonju-siRepublic of Korea
  3. 3.Department of Food Science of Animal ResourceKonkuk UniversitySeoulRepublic of Korea
  4. 4.Bigbiogen. Co., LtdAnseongRepublic of Korea
  5. 5.Division of Applied Life ScienceGyeongsang National UniversityJinjuRepublic of Korea

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