Principal Component Analysis of Stimulatory Effect of Synbiotic Combination of Indigenous Probiotic and Inulin on Antioxidant Activity of Soymilk
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The present study aimed to screen the indigenous probiotic cultures for their effect on total phenolic contents (TPC) and associated antioxidant activities in synbiotic fermented soymilk during storage. Among 16 cultures, subtractive screening was conducted based on different tests such as acidification rate and proliferation of lactic acid bacteria (LAB) on supplementation of inulin (0–20 mM) and fructooligosaccharide (0–0.45 mM). Lactobacillus paracasei CD4 was selected as potential strain after principal component analysis (PCA) of different strains with prebiotic substrates at different concentrations. The strain was used for production of synbiotic soymilk product containing 10 mM inulin. The storage study was conducted at 4 °C for 21 days. During storage, the pH, titratable acidity, TPC, antioxidant activities, and viable cell counts (VCC) were determined. The fermentation of soymilk supplemented with 10 mM inulin did not alter the VCC; however, a decrease in pH and TPC and an increase in acidity and antioxidant activity were observed (p < 0.05). In conclusion, the synbiotic supplementation of inulin in soymilk enhanced the viability of Lactobacillus paracasei CD4 and antioxidant activity during storage under refrigeration conditions.
KeywordsPrebiotic Probiotic Antioxidant activity Principal component analysis
The authors wish to thank Jaypee University of Information Technology for providing essential facilities required for this research work.
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Conflict of Interest
The authors declare that they have no conflict of interest.
- 6.Battistini C, Gullón B, Ichimura ES, Gomes AM, Ribeiro EP, Kunigk L, Moreira JU, Jurkiewicz C (2017) Development and characterization of an innovative synbiotic fermented beverage based on vegetable soybean. Braz J Microbiol 49:303–309. https://doi.org/10.1016/j.bjm.2017.08.006 CrossRefGoogle Scholar
- 13.Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158Google Scholar
- 24.Kailasapathy K, Sultana K (2009) Survival and β-galactosidase activity of encapsulated and free Lactobacillus acidophilus and Bifidobacterium lactis in ice-cream. Aust J Dairy Technol 58:223–227Google Scholar