Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 931–937 | Cite as

Effect of Dietary Supplementation of Zinc and Multi-Microbe Probiotic on Growth Traits and Alteration of Intestinal Architecture in Broiler

  • Muqader Shah
  • Hafsa ZanebEmail author
  • Saima Masood
  • Rifat Ullah Khan
  • Saima Ashraf
  • Arbab Sikandar
  • Hafiz Faseeh Ur Rehman
  • Habib Ur Rehman


This experiment aimed to investigate the effect of different levels of zinc (Zn) and a probiotic on the growth and intestinal health of broiler chickens. One hundred ninety-two-day-old broiler chicks were randomly assigned to 6 groups. The birds were supplemented with Zn (30 and 60 mg/kg) and a probiotic mixture alone or in combination with Zn. The results revealed that the broilers receiving a probiotic mixture alone or in combination with Zn (30 and 60 mg) increased (P < 0.05) final body weight, feed conversion ratio (FCR), and total goblet cell count in duodenum compared with the control group. Morphometry of the duodenum also revealed an increase (P < 0.05) in villus height (VH) and the ratio of VH and crypt depth in all the supplemented groups, whereas ileal VH increased (P < 0.05) only in Zn60 group when compared with control. Based on our results, we concluded that compared to the individual supplementation, the combined effect of Zn and probiotic mixture was superior in improving the growth performance and histomorphology of the intestine; however, the effect of two levels of Zn did not vary when combined with probiotic.


Broiler Gut morphology Probiotic Performance Zinc 



We acknowledge the Department of Poultry Production, University of Veterinary and Animal Sciences, Lahore (Pakistan) for provision of broiler experimental house and other facilities for conduction of the experimental trial.

Compliance with Ethical Standards

Conflict of Interest

We have no personal or financial linkages with the commercial organization that can affect our results. We also have no any personal interest in any product that can influence our research work.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Animal Health, Faculty of Animal Husbandry & Veterinary SciencesThe University of AgriculturePeshawarPakistan
  2. 2.Department of Anatomy and Histology, Faculty of BiosciencesUniversity of Veterinary and Animal SciencesLahorePakistan
  3. 3.Department of Physiology, Faculty of BiosciencesUniversity of Veterinary and Animal SciencesLahorePakistan

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