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Probiotics and Antimicrobial Proteins

, Volume 11, Issue 1, pp 207–219 | Cite as

Dietary Application of the Probiotic Lactobacillus plantarum 426951 Enhances Immune Status and Growth of Rainbow Trout (Oncorhynchus mykiss) Vaccinated Against Yersinia ruckeri

  • Mehdi SoltaniEmail author
  • Komael Pakzad
  • Ali Taheri-Mirghaed
  • Saeed Mirzargar
  • Seyed Pezhman Hosseini Shekarabi
  • Parasto Yosefi
  • Narges Soleymani
Article

Abstract

This study was aimed to assess the effect of oral application of Lactobacillus plantarum (2 × 107 CFU g−1 feed) as a probiotic on growth performance and immune status of vaccinated rainbow trout (29.5 ± 2 g) to yersiniosis at 16 ± 2 °C for 72 days. Fish were randomly allocated into 12 fiber glass tanks (4100 L) at a density of 80 fish per tank (240 fish per treatment). The results revealed that the activity of lysozyme and alkaline phosphatase was significantly higher in immunized fish fed with diet supplemented with probiotic (vaccine +probiotic) than that in the immunized group fed with basal diet (vaccine group) while no significant differences in levels of hematological parameters, complements, total IgM, proteins, and the intestine lactic acid bacteria (LAB) were detected. Also, significantly a better growth performance in terms of feed conversion ratio, weight gain, and thermal growth coefficient was seen in the vaccine + probiotic group than that in the vaccine group. These results indicate that feeding probiotic after vaccination can enhance the efficacy of immersion vaccination to Yersinia ruckeri.

Keywords

Lactobacillus plantarum Yersinia ruckeri Growth Trout Immune response 

Notes

Acknowledgments

The authors wish to thank Mr. Bagheri and Mr. Daryadel for their valuable assistance.

Funding Information

This work was financially supported by research grant from research council of the University of Tehran and Center of Excellence of Aquatic Animal Health, University of Tehran.

Compliance with Ethical Standards

All protocols performed in this study involving animals were in accordance with animal care and use committee of the University of Tehran and were in compliance with the guidelines for “care and use of animals for scientific purposes.”

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Mehdi Soltani
    • 1
    • 2
    Email author
  • Komael Pakzad
    • 1
  • Ali Taheri-Mirghaed
    • 1
  • Saeed Mirzargar
    • 1
  • Seyed Pezhman Hosseini Shekarabi
    • 3
  • Parasto Yosefi
    • 4
  • Narges Soleymani
    • 1
  1. 1.Department of Aquatic Animal Health, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Center of Excellence of Aquatic Animal HealthUniversity of TehranTehranIran
  3. 3.Department of Fisheries Science, Science and Research BranchIslamic Azad UniversityTehranIran
  4. 4.Central Research Laboratory, Faculty of Veterinary MedicineUniversity of TehranTehranIran

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