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A New Synthetic Peptide with In vitro Antibacterial Potential Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

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Abstract

In this work, we performed the rational design of a cationic antimicrobial peptide, GIBIMPY4, using the software DEPRAMPs developed at the GIBIM research group. GIBIMPY4 has a length of 17 amino acids, it is amphipathic, its structure is α-helix and it has a net charge of (+5). Solid-phase peptide synthesis was performed using the Fmoc strategy in acid medium. The primary structure was confirmed by MALDI-TOF mass spectrometry. The antimicrobial activity of the peptide was evaluated by broth microdilution method by measuring optical density in 96-well microplates. The minimal inhibitory concentration of GIBIMPY4 to kill 50 % of the bacterial cells (MIC50) was 6.20 ± 0.02 µM for MRSA and 4.55 ± 0.02 µM for E. coli O157:H7, while also reporting a bacteriostatic effect for the later. GIBIMPY4 activity was sensitive to salt concentration in E. coli but insignificant effect in its activity against MRSA. The peptide seems to be a broad-spectrum antimicrobial agent based on the results against Gram-positive and Gram-negative bacteria and was specific for bacterial cells E. coli O157:H7 with index of specificity equal to 9.01 in vitro assays.

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Acknowledgments

The authors would like to thank The Administrative Department of Science, Technology and Innovation, COLCIENCIAS, for resources and funding provided for the development of this research. Also to the the Mass Spectrometry Laboratory of the Industrial University of Santander by the collection of mass spectra of the synthetic peptide.

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Authors and Affiliations

  1. Laboratorio de Espectroscopia Atómica y Molecular (LEAM), Escuela de Química, Universidad Industrial de Santander, Parque Tecnológico Guatiguará, Piedecuesta, 681012, Colombia

    Y. A. Prada & E. Mejía-Ospino

  2. Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Bacteriología, Universidad Industrial de Santander, Bucaramanga, 680002, Colombia

    Y. A. Prada, P. Rondón, C. Ortíz & R. Torres

  3. Núcleo de Biotecnología Curauma, Universidad Católica de Valparaíso, 2373223, Valparaiso, Chile

    F. Guzmán

  4. Centro de Investigación en Enfermedades Tropicales (CINTROP), Escuela de Medicina, Universidad Industrial de Santander, Parque Tecnológico Guatiguará, Piedecuesta, 681012, Colombia

    P. Rondón & P. Escobar

  5. Grupo de Investigación en Control, Electrónica, Modelado y Simulación (CEMOS), Escuela de Ingeniería Eléctrica, Electrónica y Telecomunicaciones, Universidad Industrial de Santander, Bucaramanga, 680002, Colombia

    D. A. Sierra

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  1. Y. A. Prada
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  2. F. Guzmán
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  3. P. Rondón
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  4. P. Escobar
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  5. C. Ortíz
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  6. D. A. Sierra
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  7. R. Torres
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Correspondence to Y. A. Prada.

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Prada, Y.A., Guzmán, F., Rondón, P. et al. A New Synthetic Peptide with In vitro Antibacterial Potential Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA). Probiotics & Antimicro. Prot. 8, 134–140 (2016). https://doi.org/10.1007/s12602-016-9219-9

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