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Probiotics and Antimicrobial Proteins

, Volume 8, Issue 1, pp 46–52 | Cite as

Molecular Characterization of a Newly Identified Subfamily Member of Penaeidin from two Penaeid Shrimps, Fenneropenaeus indicus and Metapenaeus monoceros

  • V. V. Afsal
  • Swapna P. Antony
  • Rosamma Philip
  • I. S. Bright Singh
Article

Abstract

Penaeidins are a major group of antimicrobial peptides found in penaeid shrimps. This study reports a new isoform of penaeidin from the hemocytes of Indian white shrimp, Fenneropenaeus indicus (Fi-PEN, JX657680), and the pink shrimp, Metapenaeus monoceros (Mm-PEN, KF275674). Mm-PEN is also the first antimicrobial peptide to be identified from M. monoceros. The complete coding sequences of the newly identified Fi-PEN and Mm-PEN consisted of an ORF of 338 bp encoding 71 amino acids with a predicted molecular weight of 5.66 kDa and a pI of 9.38. The penaeidins had its characteristic signal peptide region (19 amino acids), which was followed by a mature peptide with a proline-rich domain (24 amino acids) at the N-terminal region and a cysteine-rich domain (28 amino acids) at the C-terminal region, designating it to penaeidin-3 subgroup. Structural analysis revealed an alpha-helix in its secondary structure and an extended structure at the proline-rich domain. The newly identified penaeidin isoform showed maximum similarity of 63 % to a penaeidin-3 isoform of P. monodon, which further proves it to be a new isoform. Phylogenetic analysis showed that it possessed similar evolutionary status like other penaeidins, which has subsequently diverged at different phases of evolution. The wide distribution of penaeidins in penaeid shrimps indicates the importance of these AMPs in the innate immunity.

Keywords

Antimicrobial peptide Penaeidin Innate immunity Fenneropenaeus indicus Metapenaeus monoceros 

Notes

Acknowledgments

The authors are grateful to the Ministry of Earth Sciences (MoES), Government of India, for the research Grant (MoES/10-MLR/01/2012) with which the work was carried out. Authors also thank The Co-ordinator, National Centre for Aquatic and Animal Health (NCAAH), CUSAT, for scientific support.

Funding

The study was funded by Ministry of Earth Sciences (MoES), Government of India (MoES/10-MLR/01/2012).

Compliance with Ethical Standards

Conflict of interest

Afsal V.V., Swapna P. Antony, Rosamma Philip and I.S. Bright Singh declare that they have no conflict of interest.

Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • V. V. Afsal
    • 1
  • Swapna P. Antony
    • 1
    • 2
  • Rosamma Philip
    • 1
  • I. S. Bright Singh
    • 2
  1. 1.Department of Marine Biology, Microbiology and Biochemistry, School of Marine SciencesCochin University of Science and TechnologyKochiIndia
  2. 2.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyKochiIndia

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