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Probiotics and Antimicrobial Proteins

, Volume 7, Issue 3, pp 181–192 | Cite as

Screening, Characterization and In Vitro Evaluation of Probiotic Properties Among Lactic Acid Bacteria Through Comparative Analysis

  • Sundru Manjulata Devi
  • Ann Catherine Archer
  • Prakash M. Halami
Article

Abstract

The present work aimed to identify probiotic bacteria from healthy human infant faecal and dairy samples. Subsequently, an assay was developed to evaluate the probiotic properties using comparative genetic approach for marker genes involved in adhesion to the intestinal epithelial layer. Several in vitro properties including tolerance to biological barriers (such as acid and bile), antimicrobial spectrum, resistance to simulated digestive fluids and cellular hydrophobicity were assessed. The potential probiotic cultures were rapidly characterized by morphological, physiological and molecular-based methods [such as RFLP, ITS, RAPD and (GTG)5]. Further analysis by 16S rDNA sequencing revealed that the selected isolates belong to Lactobacillus, Pediococcus and Enterococcus species. Two cultures of non-lactic, non-pathogenic Staphylococcus spp. were also isolated. The native isolates were able to survive under acidic, bile and simulated intestinal conditions. In addition, these cultures inhibited the growth of tested bacterial pathogens. Further, no correlation was observed between hydrophobicity and adhesion ability. Sequencing of probiotic marker genes such as bile salt hydrolase (bsh), fibronectin-binding protein (fbp) and mucin-binding protein (mub) for selected isolates revealed nucleotide variation. The probiotic binding domains were detected by several bioinformatic tools. The approach used in the study enabled the identification of potential probiotic domains responsible for adhesion of bacteria to intestinal epithelial layer, which may further assist in screening of novel probiotic bacteria. The rapid detection of binding domains will help in revealing the beneficial properties of the probiotic cultures. Further, studies will be performed to develop a novel probiotic product which will contribute in food and feed industry.

Keywords

Probiotics Lactic acid bacteria Binding proteins Bile salt hydrolase Phylogeny Genetic comparison 

Notes

Acknowledgments

We extend our gratitude to Prof. Ram Rajasekharan, Director, CSIR-CFTRI, Mysore, for encouragement and support. This work was carried out in the ICMR, New Delhi, funded project on probiotics (No. 5/9/1029/2011-RHN).

Conflict of interest

The authors declare no conflicts of interest.

Supplementary material

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Supplementary material 3 (DOC 42 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sundru Manjulata Devi
    • 1
  • Ann Catherine Archer
    • 1
  • Prakash M. Halami
    • 1
  1. 1.Microbiology and Fermentation Technology DepartmentCSIR- Central Food Technological Research InstituteMysoreIndia

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