Effective Treatment of Staphylococcal Scalded Skin Syndrome with Platelet Microbicidal Protein in CBRB-Rb(8.17)1Iem Mice Model
Skin and soft-tissue infections are among the most common infections. Staphylococcus aureus may cause a number of toxin-mediated diseases, including staphylococcal scalded skin syndrome (SSSS). The therapeutic efficacy of some antimicrobial peptides was recently evaluated in a mouse model of SSSS. This study is the first in vivo demonstration of the use of PMP to improve outcome of SSSS. Twenty-four CBRB-Rb(8.17)1Iem female mice naturally infected by endogenous S. aureus with SSSS symptoms were used in this work and divided into two equal groups. From neck of each mouse was isolated and identified endogenous exfoliative producing strain of S. aureus. PMP was obtained from human platelets and tested against Bacillus subtilis ATCC 6633. PMP had bactericidal activity against B. subtilis ATTC 6633 and endogenous strain of S. aureus at 2.0 ± 0.5 and 14.5 ± 0.5 µg/ml, respectively. At 4 weeks, the mice of experimental group were treated subcutaneous near exfoliative zone with 0.2 ml of PMP in final concentration 10 µg/ml every day. Control mice was injected with 0.2 ml 0.9 % NaCl. At 1 day of experiment maximal zone of alopecia was at PMP-treating group (380 ± 20 mm2) in comparison with control group (167 ± 10 mm2, p < 0.01). At 50 day of observation (22nd day after the end of treatment), the square of alopecia in control group was 1220 ± 40 mm2 in comparison with 870 ± 17 mm2 in experimental group (p < 0.01). The antistaphylococcal in vivo activity of PMP demonstrated in present study makes these molecules potentially useful for treatment of SSSS.
KeywordsStaphylococcal scalded skin syndrome Mice model Platelet microbicidal protein Treatment
Research was done with financial support of the Russian Science Foundation (Grant No. 14-36-00023).
Conflict of interest
The authors declare no conflict of interest.
- 1.Afshar M, Gallo RL (2013) Innate immune defense of the skin. Vet Dermatol 24:32–38.e8–9. doi: 10.1111/j.1365-3164.2012.01082.x
- 3.Brook I (2002) Secondary bacterial infections complicating skin lesions. J Med Microbiol 51:808–812Google Scholar
- 5.Ivanov IB (2005) In vitro resistance to human platelet microbicidal protein among urethral staphylococcal and enterococcal isolates with its correlation with prostatitis. Indian J Med Microbiol 23:253–255Google Scholar
- 9.Kupferwasser LI, Skurray RA, Brown MH et al (1999) Plasmid-mediated resistance to thrombin-induced platelet microbicidal protein in staphylococci: role of the qacA locus. Antimicrob Agents Chemother 43:2395–2399Google Scholar
- 12.Moiseeva EV (2005) Original approaches to test anti-breast cancer drugs in a novel set of mouse models. Ph D Thesis, Utrecht UniversityGoogle Scholar
- 14.Noguchi N, Nakaminami H, Nishijima S et al (2006) Antimicrobial agent of susceptibilities and antiseptic resistance gene distribution among methicillin-resistant Staphylococcus aureus isolates from patients with impetigo and staphylococcal scalded skin syndrome. J Clin Microbiol 44:2119–2125CrossRefGoogle Scholar