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Probiotics and Antimicrobial Proteins

, Volume 6, Issue 2, pp 114–121 | Cite as

Functional Characterization of a c-type Lysozyme from Indian Shrimp Fenneropenaeus indicus

  • Viswanathan Karthik
  • Vijayan Kamalakannan
  • Ancy Thomas
  • Naduvilamuriparambu Saidumuhammed Sudheer
  • Issac S. Bright Singh
  • Rangarajan Badri Narayanan
Article

Abstract

Lysozyme gene from Fenneropenaeus indicus was cloned, expressed in Escherichia coli and characterized. The cDNA consists of 477 base pairs and encodes amino acid sequence of 159 residues. F. indicus lysozyme had high identity (98 %) with Fenneropenaeus merguiensis and Fenneropenaeus chinensis and exhibits low to moderate identities with lysozymes of other invertebrates and vertebrates. This lysozyme is presumed to be chicken types as it possesses two catalytic and eight cysteine residues that are conserved across c-type lysozymes and a c-terminal extension, which is a characteristic of lysozymes from marine invertebrates. Further, the antimicrobial properties of the recombinant lysozyme from F. indicus were determined in comparison with recombinant hen egg white lysozyme. This exhibited high activity against a Gram-negative pathogenic bacterium Salmonella typhimurium and two fungal strains Pichia pastoris and Saccharomyces cerevisiae in turbidimetric assay. Distribution of lysozyme gene and protein in tissues of shrimps infected with white spot syndrome virus revealed that the high levels of lysozyme are correlated with low and high viral load in abdominal muscle and tail, respectively. In conclusion, lysozyme from F. indicus has a broad spectrum of antimicrobial properties, which once again emphasizes its role in shrimp innate immune response.

Keywords

Lysozyme WSSV Fenneropenaeus indicus Gene expression 

Notes

Acknowledgments

This work is supported by the grant from the Department of Biotechnology, Government of India, New Delhi. We wish to thank Dr. S. Rajesh for his help in cloning this gene.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Viswanathan Karthik
    • 1
  • Vijayan Kamalakannan
    • 1
  • Ancy Thomas
    • 1
  • Naduvilamuriparambu Saidumuhammed Sudheer
    • 2
  • Issac S. Bright Singh
    • 2
  • Rangarajan Badri Narayanan
    • 1
  1. 1.Centre for BiotechnologyAnna UniversityChennaiIndia
  2. 2.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyCochinIndia

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