Anti-listerial Bactericidal Activity of Lactobacillus plantarum DM5 Isolated from Fermented Beverage Marcha
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The strain Lactobacillus plantarum DM5 was isolated from fermented beverage Marcha of Sikkim and explored for its antagonistic activity against food-borne pathogens. The cell-free supernatant of L. plantarum DM5 showed antibacterial activity of 6,400 AU/mL in MRS medium (pH 6.0) against the indicator strain Staphylococcus aureus. MRS medium supplemented with 15 g/L of maltose at 37 °C under static condition yielded highest antimicrobial activity (6,400 AU/mL) with 3 % increase in specific activity when compared to 20 g/L glucose. The antimicrobial compound was heat stable (60 min at 100 °C) and was active over a wide pH range. It showed bactericidal effect on S. aureus and Listeria monocytogenes by causing 96 and 98 % of cell lysis, respectively. The cell morphology of the treated S. aureus and L. monocytogenes was completely deformed as revealed by scanning electron microscopy, suggesting the high potential of L. plantarum DM5 as natural preservatives in food industry. The antimicrobial compound was purified by 80 % ammonium sulphate precipitation and showed antimicrobial activity of 12,800 AU/mL with 19-fold purification and a molecular mass of 15.2 kDa, indicating the proteinaceous nature of the compound.
KeywordsLactobacillus plantarum Antagonistic activity Pathogens Marcha
The research work was supported by a project grant from Department of Biotechnology (DBT), New Delhi, India, to A. Goyal. The fellowship of D. Das was supported by Council of Industrial and Scientific Research (CSIR), New Delhi, India. The authors are thankful to the Indian Institute of Technology, Guwahati, India, for providing the research facilities.
Conflict of interest
Arun Goyal has received the project grant for this research from Department of Biotechnology (DBT), New Delhi, India; Deeplina Das has received fellowship from by Council of Industrial and Scientific Research (CSIR), New Delhi, India; the authors certify that there is no other conflict of interest with any other financial organization regarding this research.
This article does not contain any studies with human or animal subjects. The indicator strains used in this study were procured from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India.
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