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Probiotics and Antimicrobial Proteins

, Volume 5, Issue 2, pp 99–109 | Cite as

Quantitative Appraisal of the Probiotic Attributes and In Vitro Adhesion Potential of Anti-listerial Bacteriocin-producing Lactic Acid Bacteria

  • Sandipan Mukherjee
  • Atul Kumar Singh
  • Manab Deb Adhikari
  • Aiyagari RameshEmail author
Article

Abstract

Estimation of bile tolerance, endurance to gastric and intestinal environment and adhesion potential to intestinal cells are significant selection criteria for probiotic lactic acid bacteria (LAB). In this paper, the probiotic potential of native bacteriocin-producing LAB isolated previously from indigenous source has been determined through quantitative approaches. Among fifteen anti-listerial bacteriocin-producing native LAB, ten strains were found to be bile tolerant. The presence of bile salt hydrolase (bsh) gene in native Lactobacillus plantarum strains was detected by PCR and confirmed by nucleic acid sequencing of a representative amplicon. Interestingly, three native LAB strains exhibited significant viability in simulated gastric fluid, analogous to the standard LAB Lactobacillus rhamnosus GG, while an overwhelming majority of the native LAB strains demonstrated the ability to survive and remain viable in simulated intestinal fluid. Quantitative adhesion assays based on conventional plating method and a fluorescence-based method revealed that the LAB isolates obtained from dried fish displayed significant in vitro adhesion potential to human adenocarcinoma HT-29 cells, and the adhesion level was comparable to some of the standard probiotic LAB strains. The present study unravels putative probiotic attributes in certain bacteriocin-producing LAB strains of non-human origin, which on further in vivo characterization could find specific applications in probiotic food formulations targeted for health benefits.

Keywords

Lactic acid bacteria Probiotic Adhesion Bacteriocin 

Notes

Acknowledgments

This work was supported by a research grant from Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India [No. 38(1251)/10/EMR-II]. We thank the National Facility of Automated DNA Sequencing, Department of Biochemistry, Delhi University, South Campus for their support in nucleic acid sequencing.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sandipan Mukherjee
    • 1
  • Atul Kumar Singh
    • 1
  • Manab Deb Adhikari
    • 1
  • Aiyagari Ramesh
    • 1
    Email author
  1. 1.Department of BiotechnologyIndian Institute of Technology GuwahatiGuwahatiIndia

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