Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection
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Venom of the ant spider Lachesana tarabaevi contains a wide variety of antimicrobial peptides. Among them, a special place belongs to cyto-insectotoxins, a class of cytolytic molecules showing equally potent antimicrobial and insecticidal effects. We tested one of them, CIT 1a, for ability to suppress Chlamydia trachomatis infection. HEK293 cells were transfected with plasmid vectors harboring the cit 1a gene. Controlled expression of the transgene led to a significant decrease in C. trachomatis viability inside the infected cells. Using proteomic and transcriptomic approaches, we found alterations in protein expression patterns and identified differentially expressed genes in transfected cells.
KeywordsAntimicrobial peptide Cytolytic peptide Infectious disease Gene therapy Proteomics Transcriptome
We are grateful to Irina Demina and Maria Galyamina from the Research Institute for Physico-Chemical Medicine, Moscow, for proteomic experiments. We thank Dr. Eva Hjelm (Uppsala University, Sweden) for the provided Chlamydia strain. Microarray experiments were carried out at ZAO “Genoanalytica”, Moscow, Russia. This work was funded by the Russian Foundation for Basic Research (grant number 11-04-00706) and the Program of Cell and Molecular Biology of the Russian Academy of Sciences.
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