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Probiotics and Antimicrobial Proteins

, Volume 4, Issue 2, pp 122–132 | Cite as

Fluorescence-Based Comparative Evaluation of Bactericidal Potency and Food Application Potential of Anti-listerial Bacteriocin Produced by Lactic Acid Bacteria Isolated from Indigenous Samples

  • Atul Kumar Singh
  • Sandipan Mukherjee
  • Manab Deb Adhikari
  • Aiyagari RameshEmail author
Article

Abstract

The aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (LAB) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. A total of 231 LAB isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. The anti-listerial LAB were identified by PCR as Lactobacillus sp., Pediococcus sp., Enterococcus sp., and Lactococcus sp. PCR also enabled the detection of Class IIa bacteriocin-encoding genes such as enterocin A, pediocin, and plantaricin A in some of the LAB isolates. The culture filtrate from anti-listerial LAB isolates demonstrated bacteriocin-like inhibitory substance (BLIS) against common Gram-positive pathogenic bacteria such as Staphylococcus aureus, Enterococcus faecalis, and Bacillus cereus, and partial characterization of BLIS confirmed the production of bacteriocin by the LAB isolates. Sensitive fluorescence-based assays employing specific probes indicated the comparative potencies of the bacteriocin and clearly revealed the membrane-targeted anti-listerial activity of the purified bacteriocin produced by selected LAB isolates. The food application potential of plantaricin A produced by a native isolate Lactobacillus plantarum CRA52 was evidenced as the bacteriocin suppressed the growth of Listeria monocytogenes Scott A inoculated in paneer samples that were stored at 8 °C for 5 days.

Keywords

Lactic acid bacteria Bacteriocin Membrane damage Listeria monocytogenes Paneer 

Notes

Acknowledgments

We thank the Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India for a research grant [No. 38(1251)/10/EMR-II]. We thank the National Facility of Automated DNA Sequencing, Department of Biochemistry, Delhi University, South campus for their support in nucleic acid sequencing.

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Copyright information

© Springer Science + Business Media, LLC 2012

Authors and Affiliations

  • Atul Kumar Singh
    • 1
  • Sandipan Mukherjee
    • 1
  • Manab Deb Adhikari
    • 1
  • Aiyagari Ramesh
    • 1
    Email author
  1. 1.Department of BiotechnologyIndian Institute of Technology GuwahatiGuwahatiIndia

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