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Probiotics and Antimicrobial Proteins

, Volume 2, Issue 3, pp 186–196 | Cite as

Probiotic Lactobacillus and Bifidobacterial Lectins Against Candida albicans and Staphylococcus aureus Clinical Strains: New Class of the Pathogen Biofilm Destructors

  • M. Lakhtin
  • V. Alyoshkin
  • V. LakhtinEmail author
  • S. Afanasyev
  • L. Pozhalostina
  • V. Pospelova
Article

Abstract

Preparations of probiotic bifidobacterial and lactobacillus lectins possessed system affinity to mannan and mucin-type polymers. It was shown that these lectins possess fungistatic and fungicidal activities against nystatin-resistant Candida albicans clinical strains. Lectins revealed destructive properties with respect to C. albicans and Staphylococcus aureus biofilms, depending on clinical strain origin and lectin preparation type. Synergistic antipathogen activities between lectins and between lectins and nystatin were observed. In the presence of lectins, pathogen biofilm degradation occurred in sequential steps, including biofilm refinement, appearance of edge cavities, segmentation, detachment of fragments and their lysis. Fungal response to lectins was more complex compared to that of staphylococci. Cold stress improved pictures of lectin antipathogen action. The data indicate that probiotic bacterial lectins are members of a new class of antimicrobials—destructors of pathogen biofilms.

Keywords

Probiotics Lectins Antimicrobials Synergistic action Candida albicans Staphylococcus aureus Clinical strains Biofilms 

Abbreviations

aLL

Acidic lectins of lactobacilli

aLB

Acidic lectins of bifidobacteria

bLL

Basic lectins of lactobacilli

bLB

Basic lectins of bifidobacteria

RPMI-HEPES

RPMI-1640 plus HEPES pH 7.2–7.4

PAA(b)

Polyacrylamide (biotinylated)

PBS

Phosphate saline buffer pH 7.2–7.4

PHA

Phytohaemagglutinin

Notes

Acknowledgments

The authors thank Prof. Chikindas and all other reviewers for fruitful discussion and value comments and recommendations. The authors also thank Katia Noll Sutyak for the technical help in preparing this article.

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Copyright information

© Springer Science + Business Media, LLC 2010

Authors and Affiliations

  • M. Lakhtin
    • 1
  • V. Alyoshkin
    • 1
  • V. Lakhtin
    • 1
    Email author
  • S. Afanasyev
    • 1
  • L. Pozhalostina
    • 1
  • V. Pospelova
    • 1
  1. 1.G.N. Gabrichevsky Research Institute for Epidemiology and MicrobiologyMoscowRussia

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